11 research outputs found

    Zaštitno djelovanje selenija protiv prekomjerne ekspresije apoptotskih gena povezanih s karcinomom u štakora izloženih o-krezolu

    Get PDF
    Cresols are monomethyl derivatives of phenol frequently used as solvents and intermediates in the production of disinfectants, fragrances, pesticides, dyes, and explosives, which is probably why they are widely distributed in the environment. General population may be exposed to cresols mainly through inhalation of contaminated air. In this study we evaluated the toxicological effects of o-cresol on differential gene expression profile of rat liver and prostate. Experiments were conducted on 80 male rats, 60 of which were exposed to o-cresol (1.5 g kg-1, 5 g kg-1, or 15 g kg-1) through feed for 8 weeks. Three groups of rats were supplemented with 0.1 mg kg-1 selenium (Se, in the form of, sodium selenite) in addition to o-cresol to evaluate its effectiveness against o-cresol toxicity. Control group received neither o-cresol nor Se, while one group received Se alone. Survival was similar between the exposed and control animals. Rats exposed to 15 g kg-1 of o-cresol showed a 16 % loss in body weight by the end of the study, which may have been related to o-cresol making feed unpalatable at this concentration. Liver and prostate tissue samples were collected at the end of the treatment. mRNA analysis revealed that apoptotic genes (CYP3A, COX-2, PPARγ, BAX, BCL2, AKT-1, and PKCα) related to cancer were up-regulated in liver and prostate tissues isolated from groups exposed to 5 g kg-1 and 15 g kg-1 o-cresol in comparison to control. Changes in gene expression profile were prevented when rats were supplemented with Se. The exact mechanisms underlying its protective effect remain to be clarified by future studies.Krezoli su monometilni derivati fenola koji se često rabe kao otapala te kao posrednici u proizvodnji dezinfekcijskih sredstava, mirisa, pesticida, boja i eksploziva. Otuda i njihova rasprostranjenost u okolišu. Opća je populacija izložena krezolima uglavnom putem zraka. U ovome se toksikološkom istraživanju ocijenilo djelovanje o-krezola, jednoga od tri krezolova izomera, na ekspresiju gena u tkivima jetre i prostate mužjaka štakora. Istraživanje je provedeno na 80 mužjaka, od kojih je 60 tijekom osam tjedana bilo izloženo o-krezolu (1,5 g kg-1, 5 g kg-1, odnosno 15 g kg-1) preko krmiva. Tri skupine štakora primale su uz o-krezol nadomjestak selenija u dozi od 0.1 mg kg-1 (Se, u obliku natrijeva selenita) radi ocjene njegove djelotvornosti protiv toksičnosti o-krezola. Kontrolna skupina nije primala ni o-krezol ni Se, dok je jedna skupina primala samo Se. Preživljenje je bilo podjednako u svih skupina životinja. Štakori izloženi najvišoj dozi o-krezola (15 g kg-1) imali su 16 % manju tjelesnu masu od kontrolne skupine na kraju ispitivanja, što može biti povezano s lošim okusom krmiva zbog primjese visoke doze o-krezola. S istekom osmotjednoga izlaganja o-krezolu životinje su eutanazirane te su prikupljeni uzorci tkiva jetre i prostate. Analiza m-RNA pokazala je značajno povišenu ekspresiju apoptotskih gena CYP3A, COX-2, PPARγ, BAX, BCL2, AKT-1 i PKCα, koji su povezani s nastankom karcinoma u skupinama štakora izloženim o-krezolu (5 g kg-1 i 15 g kg-1 u odnosu na kontrolu. Ova je prekomjerna ekspresija poništena u štakora koji su primali selenij. Još nisu jasni mehanizmi iza ovoga zaštitnog djelovanja, na što će odgovoriti buduća istraživanja

    Disc-based microarrays: principles and analytical applications

    Full text link
    [EN] The idea of using disk drives to monitor molecular biorecognition events on regular optical discs has received considerable attention during the last decade. CDs, DVDs, Blu-ray discs and other new optical discs are universal and versatile supports with the potential for development of protein and DNA microarrays. Besides, standard disk drives incorporated in personal computers can be used as compact and affordable optical reading devices. Consequently, a CD technology, resulting from the audio-video industry, has been used to develop analytical applications in health care, environmental monitoring, food safety and quality assurance. The review presents and critically evaluates the current state of the art of disc-based microarrays with illustrative examples, including past, current and future developments. Special mention is made of the analytical developments that use either chemically activated or raw standard CDs where proteins, oligonucleotides, peptides, haptens or other biological probes are immobilized. The discs are also used to perform the assays and must maintain their readability with standard optical drives. The concept and principle of evolving disc-based microarrays and the evolution of disk drives as optical detectors are also described. The review concludes with the most relevant uses ordered chronologically to provide an overview of the progress of CD technology applications in the life sciences. Also, it provides a selection of important references to the current literature.This work was supported by the Spanish Ministry of Economy and Competitiveness (project CTQ2013-45875-R) and Generalitat Valenciana (PROMETEO II 2014/040 and ACOMP 2012/158). All authors were partially sponsored by the European Regional Development Fund.Morais, S.; Puchades, R.; Maquieira Catala, Á. (2016). Disc-based microarrays: principles and analytical applications. Analytical and Bioanalytical Chemistry. 408(17):4523-4534. https://doi.org/10.1007/s00216-016-9423-1S4523453440817Petryayeva E, Algar WR. RSC Adv. 2015;5:22256–82.Ozcan A. Lab Chip. 2014;14:3187–94.Yu HZ, Li Y, Ou LML. Acc Chem Res. 2013;46:258–68.Morais S, Tortajada-Genaro L, Maquieira A. Expert Rev Mol Diagn. 2014;14:773–5.Brahima Sanou, ICT Data and Statistics Division (2015) The world in 2015. http://www.itu.int/en/ITU-D/Statistics/Documents/facts/ICTFactsFigures2015.pdfContreras-Naranjo JC, Wei Q, Ozcan A. IEEE J Sel Top Quantum Electron. 2016;22:7100414.International Video Federation. European video vearbook 2014. Brussels: International Video Federation; 2014.Future market insights (2015) Recordable optical disc market– blu-ray disc is to be reckoned with new technologies due to demand from media and entertainment: global industry analysis and opportunity assessment 2015–2025. http://www.futuremarketinsights.com/reports/recordable-optical-disc-marketHattori Masakatsu, Suzuki Hiroshi, Sugaya Seiichi (2015) Trends in Technologies for HDDs, ODDs, and SSDs, and Toshiba’s Approach. http://toshiba.semicon-storage.com/product/storage/pdf/ToshibaReview_vol66n8_02.pdfEkins RP, Chu FW. Clin Chem. 1991;37:1955–67.Bañuls MJ, Morais S, Tortajada-Genaro LA, Maquieira A. Microarray technology: methods and applications. In: Li PCH, Sedighi A, Wang L, editors. Methods in molecular biology, vol. 1368. New York: Springer; 2016. p. pp 37–51.Kido H, Maquieira A, Hammock BD. Anal Chim Acta. 2000;411:1–11.La Clair JJ, Burkart MD. Org Biomol Chem. 2003;1:3244–9.Wang Z, Li RX (2007) Nanoscale Res Lett (2007) 2:69-74Li Y, Wang Z, Ou LML, Yu HZ. Anal Chem. 2007;79:426–33.Bañuls MJ, García-Piñón F, Puchades R, Maquieira A. Bioconjug Chem. 2008;19:665–72.Tamarit-López J, Morais S, Bañuls MJ, Puchades R, Maquieira A. Anal Chem. 2010;82:1954–63.Tamarit-López J, Morais S, Puchades R, Maquieira A. Bioconjug Chem. 2011;22:2573–80.Arai T, Gopinath SCB, Mizuno H, Kumar PKR, Rockstuhl C, Awazu K, et al. Jpn J Appl Phys. 2007;46:4003–6.Gopinath SCB, Awazu K, Tominaga J, Kumar PKR. ACS Nano. 2008;2:1885–95.Brun EM, Puchades R, Maquieira A. Anal Chem. 2013;85:4178–86.Tamarit-López J, Morais S, Puchades R, Maquieira A. Anal Chim Acta. 2008;609:120–30.Song Y, Luo D, Ye S, Hou H, Wang L. Appl Surf Sci. 2012;258:2584–90.Barrios CA, Canalejas-Tejeros V, Herranz S, Moreno-Bondi MC, Avella-Oliver M, Puchades R, et al. Plasmonics. 2014;9:645–9.Barrios CA, Canalejas-Tejeros V. Nanoscale. 2015;7:3435–9.Peris E, Bañuls MJ, Puchades R, Maquieira A. J Mater Chem B. 2013;1:6245–53.Arnandis-Chover T, Morais S, Tortajada-Genaro LA, Puchades R, Maquieira A, Berganza J, et al. Talanta. 2012;101:405–12.Dobosz P, Morais S, Puchades R, Maquiera A. Biosens Bioelectron. 2015;69:294–300.Dobosz P, Morais S, Puchades R, Maquiera A. Anal Chem. 2015;87:9817–24.Morais S, Tamarit-López J, Carrascosa J, Puchades R, Maquieira A. Anal Bioanal Chem. 2008;391:2837–44.Potyrailo RA, Morris WG, Leach AM, Sivavec TM, Wisnudel MB, Boyette S. Anal Chem. 2006;78:5893–9.Yamaji K, Takase M (1990) Idemitsu Petrochemical co., Ltd., Japan, 1990 CA2014294A1, Canada.Challener WA, Ollmann RR (1998) US Patent 5994150.Gordon JF (1994) European Patent EP 0 782 705 81Mian A, Kieffer-Higgings S G, Corey GD (1998) Patent WO97/21090Virtanen J (1998) US Patent 6030581Pallapa M, Oua LML, Parameswaran M, Yu HZ. Sens Actuators, B. 2010;148:620–3.Ivanov YD, Pleshakova TO, Krohin NV, Kaysheva AL, Usanov SA, Archakov AI. Biosens Bioelectron. 2013;43:384–90.Alexandre I, Houbion Y, Collet J, Hamels S, Demarteau J, Gala JL, et al. Biotechniques. 2002;33:435–9.Barathur R, Bookout J, Sreevatsan S, Gordon J, Werner M, Thor G, et al. Psychiatr Genet. 2002;12:193–206.Lange SA, Roth G, Wittemann S, Lacoste T, Vetter A, Grassle J, et al. Angew Chem Int Ed. 2006;45:270–3.Park KH, Lee SQ, Kim EK, Moon SE, Cho YH, Gokarna A, et al. Ultramicroscopy. 2008;108:1319–24.Kim KH, Lee SY, Kim S, Jeong SG. Curr Appl Phys. 2008;8:687–91.Donolato M, Anunes P, Zardán-Gómez de la Torre T, Hwuc E, Chen CH, Burger R, et al. Biosens Bioelectron. 2015;67:649–55.Nolte DD. Rev Sci Instrum. 2009;80:101101.http://www.gyros.comhttp://www.quanterix.com/http://www.pall.comMorais S, Marco-Moles R, Puchades R, Maquieira A. Chem Commun. 2006;22:2368–70.Morais S, Carrascosa J, Mira D, Puchades R, Maquieira A. Anal Chem. 2007;79:7628–35.Li X, Shi M, Cui C, Yu HZ. Anal Chem. 2014;86:8922–6.Gopinath SCB, Awazu K, Fons P, Tominaga J. Anal Chem. 2009;81:4963–70.Morais S, Tortajada-Genaro L.A., Arnandis-Chover T, Puchades R, Maquieira A. Anal Chem. 2009;81:5646–54.Potyrailo RA, Morris WG, Wroczynski R, Hassib L, Miller P, Dworken B, et al. Sens Actuators, B. 2009;136:203–8.Morais S, Tamarit-López J, Puchades R, Maquieira A. Environ Sci Technol. 2010;44:9024–9.Tortajada-Genaro LA, Santiago-Felipe S, Morais S, Gabaldón JA, Puchades R, Maquieira A. J Agric Food Chem. 2012;60:36–43.Bañuls MJ, Gonzalez-Pedro V, Puchades R, Maquieira A. Anal Methods. 2012;4:3133–9.Avella-Oliver M, Gimenez-Romero D, Morais S, Gonzalez-Martinez MA, Bueno PR, Puchades R, et al. Chem Commun. 2013;49:10868–70.Morais S, Maquieira A, Puchades R, Tortajada-Genaro LA. Patent WO2013/135933 A1Ramachandraiah H, Amasia M, Cole J, Sheard P, Pickhaver S, Walker C, et al. Lab Chip. 2013;13:1578–85.Arandis-Chover T, Morais S, Gonzalez-Martinez MA, Puchades R, Maquieira A. Biosens Bioelectron. 2014;51:109–14.Avella-Oliver M, Morais S, Carrascosa J, Puchades R, Maquieira A. Anal Chem. 2014;86:12037–46.Avella-Oliver M, Morais S, Carrascosa J, Puchades R, Maquieira A (2015) Patent PCT/ES2015/070060Santiago-Felipe S, Tortajada-Genaro LA, Morais S, Puchades R, Maquieira A. Sens Actuators, B. 2014;204:273–81.Santiago-Felipe S, Tortajada-Genaro LA, Morais S, Puchades R, Maquieira A. Food Chem. 2015;174:509–15.Tortajada-Genaro LA, Santiago-Felipe S, Amasia M, Russom A, Maquieira A. RSC Adv. 2015;5:29987–95.Zhang L, Wong JXH, Li X, Li Y, Yu HZ. Anal Chem. 2015;87:5062–7.Zhang L, Li X, Li Y, Shi X, Yu HZ. Anal Chem. 2015;87:1896–902.Santiago-Felipe S, Tortajada-Genaro LA, Carrascosa J, Puchades R, Maquieira A. Biosens Bioelectron. 2016;79:300–6.Avella-Oliver M, Morais S, Puchades R, Maquieira A (2015) Trends Anal Chem doi: 10.1016/j.trac.2015.11.02

    Simple and rapid sample preparation system for the molecular detection of antibiotic resistant bacteria in human urine

    Get PDF
    Antibiotic resistance in urinary tract infections (UTIs) can cause significant complications without quick detection and appropriate treatment. . We describe a new approach to capture, concentrate and prepare amplification-ready DNA from antibiotic resistant bacteria in human urine samples. Klebsiella pneumoniae NCTC13443 (blaCTX-M-15 positive) spiked into filtered human urine was used as a model system. Bacteria were captured using anion exchange diaethylaminoethyl (DEAE) magnetic microparticles and concentrated 200-fold within ~3.5 minutes using a custom, valve-less microfluidic chip. Eight samples were processed in parallel, and DNA, was released using heat lysis from an integrated resistive heater. The crude cell lysate was used for real time Recombinase Polymerase Amplification (RPA) of the blaCTX-M-15 gene. The end to end processing time was approximately 15 minutes with a limit of detection of 1000 bacteria in 1 mL urin
    corecore