20 research outputs found

    Investigating the Response to Meristem Culture of Four Regional Sweetpotato Varieties in the Western Highlands of Cameroon

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    In the Western highlands of Cameroon where sweetpotato (Ipomoea batatas L.) is an inevitable staple crop during the dry season, farmers cherish local which are presently under the risk of extinction due to accumulation of pest and diseases. Guaranteeing sustainable Sweetpotato production in this region therefore requires providing farmers with disease free planting materials such as tissue cultures. This study was conducted to investigate the feasibility of introducing disease free planting materials from meristem culture of EX-KOLB, EX-GUZANG2, EX-WIDIKUM3 and EX-NKAMBE1 which are four regional sweetpotato varieties common in the Western highlands of Cameroon. Test tubes containing growing media and meristems from each of the varieties were labeled and laid out in a completely randomized experimental design (CRD) with four replications and four treatments. Data on the number of sprouted meristems, number of rooted meristems, number of meristems contaminated, number of meristems that died, average heights of developed plants, average number of nodes on the developed plants, and average number of leaves on the developed plants was collected over a period of 8 weeks at 2 weeks interval. Meristems from all the landraces sprouted in the media used and the differences in the mean number of sprouted meristem between the landraces were significant (P0.05).This genotype-dependent response to media used for in vitro regeneration by plants has been observed by many studies. However meristems from EX-GUZANG2 and EX-KOLB showed greater positive respond to this media and merit further studies for the purpose of this experiment

    The built environment and health in low- and middle-income countries : A review on quantitative health impact assessments

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    Purpose of Review Features and attributes of the built environment (BE) impact positively and negatively on health, especially in cities facing unprecedented urban population growth and mass motorization. A common approach to assess the health impacts of built environment is health impact assessment (HIA), but it is rarely used in low- and middle-income countries (LMICs) where urbanization rates are fastest. This article reviews selected HIA case studies from LMICs and reports the methods and tools used to support further implementation of quantitative HIAs in cities of LMICs. Recent Findings In total, 24 studies were reviewed across Algeria, Brazil, China, India, Iran, Kenya, Thailand, Turkey, and Mauritius. HIAs examine specific pathways through which the built environment acts: air pollution, noise, physical activity, and traffic injury. Few HIAs of BE addressed more than one exposure pathway at a time, and most studies focused on air pollution across the sectors of transport and energy. A wide number of tools were used to conduct exposure assessment, and different models were applied to assess health impacts of different exposures. Those HIAs rely on availability of local concentration data and often use models that have set exposure–response functions (ERFs). ERFs were not adapted to local populations except for HIAs conducted in China. Summary HIAs of BE are being successfully conducted in LMICs with a variety of tools and datasets. Scaling and expanding quantitative health impact modeling in LMICs will require further study on data availability, adapted models/tools, low technical capacity, and low policy demand for evidence from modeling studies. As case studies with successful use of evidence from modeling emerge, the uptake of health impact modeling of BE is likely to increase in favor of people and planet

    Investigation of water sources as reservoirs of <i>Vibrio cholerae</i> in Bepanda, Douala and determination of physico-chemical factors maintaining its endemicity

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    Cholera remains a significant cause of mortality in developing countries. Outbreaks of the disease are associated with poverty, lack of potable water and poor sanitation. The survival and persistence of Vibrio cholerae in water has been shown to depend on physico-chemical factors. We studied water sources in Bepanda, an overcrowded neighbourhood in Douala, Cameroon, with limited access to portable water and very poor sanitary conditions as reservoirs of V. cholerae. We analysed 318 samples from various sources (well, tap, stream) from February to July 2009 using standard microbiological techniques and characterised isolates serologically using the polyvalent O1/O139 antisera. Susceptibility to antibiotics previously used for cholera treatment in Douala was studied using the disk diffusion method. Physico-chemical factors (temperature, pH and salinity) that could maintain the endemicity of the organism were analysed using standard methods. Eighty-seven (27.4%) samples were contaminated, with high isolation rates being obtained from streams (52.4%) and wells (29.8%). The number of isolates was significantly higher (P < 0.05) in the rainy season (35.5%). We detected 23 (24%) O1 serogroup isolates in streams and wells, whilst 64 (66.6%) were non-O1/non-O139. Temperature and salinity correlated positively with the occurrence of the organisms. All isolates were susceptible to fluoroquinolones but high resistance rates to trimethoprim or sulfamethozaxole and tetracycline were observed. Vibrio cholerae is endemic in Bepanda with O1 and non-O1/non-O139 serogroups co-existing in the streams and wells hence the possibility of future outbreaks of cholera if sanitation and drinking water quality are not improved. Temperature and salinity are amongst the factors maintaining the endemicity of the organism
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