16 research outputs found
Chondroitin Sulfate-Based Biomineralizing Surface Hydrogels for Bone Tissue Engineering
Chondroitin
sulfate (CS) is the major component of glycosaminoglycan
in connective tissue. In this study, we fabricated methacrylated PEGDA/CS-based
hydrogels with varying CS concentration (0, 1, 5, and 10%) and investigated
them as biomineralizing three-dimensional scaffolds for charged ion
binding and depositions. Due to its negative charge from the sulfate
group, CS exhibited an osteogenically favorable microenvironment by
binding charged ions such as calcium and phosphate. Particularly,
ion binding and distribution within negatively charged hydrogel was
dependent on CS concentration. Furthermore, CS dependent biomineralizing
microenvironment induced osteogenic differentiation of human tonsil-derived
mesenchymal stem cells in vitro. Finally, when we transplanted PEGDA/CS-based
hydrogel into a critical sized cranial defect model for 8 weeks, 10%
CS hydrogel induced effective bone formation with highest bone mineral
density. This PEGDA/CS-based biomineralizing hydrogel platform can
be utilized for in situ bone formation in addition to being an investigational
tool for in vivo bone mineralization and resorption mechanisms