Investigation of turbidity effect in two emulsions

Thesis (M.A.)--Boston UniversityThis thesis is an attempt at an original investigation of the turbidity effect in emulsions. Two types of emulsions were used, tvpe 548 which is used mainly in spectroscopic and scientific work, and type Pan-X which is used mainly in commerical and amateur photography. They are both compsed of fine grains, 548 being finer than Pan-X,however. By partially shielding them with a knife edge, which was composed of stripping film, they were both exposed to a square root of two step wedge with the use of light of tungsten quality. They were then develoved using the same procedure of development for both

Hepatitis C virus production requires apolipoprotein A-I and affects its association with nascent low-density lipoproteins

Background/aims The life cycle of hepatitis C virus (HCV) is intimately linked to the lipid metabolism of the host. In particular, HCV exploits the metabolic machinery of the lipoproteins in several steps of its life cycle such as circulation in the bloodstream, cell attachment and entry, assembly and release of viral particles. However, the details of how HCV interacts with and influences the metabolism of the host lipoproteins are not well understood. A study was undertaken to investigate whether HCV directly affects the protein composition of host circulating lipoproteins. Methods A proteomic analysis of circulating very low-, low- and high-density lipoproteins (VLDL, LDL and HDL), isolated from either in-treatment naive HCV-infected patients or healthy donors (HD), was performed using two-dimensional gel electrophoresis and tandem mass spectrometry (MALDI-TOF/TOF). The results obtained were further investigated using in vitro models of HCV infection and replication. Results A decreased level of apolipoprotein A-I (apoA-I) was found in the LDL fractions of HCV-infected patients. This result was confirmed by western blot and ELISA analysis. HCV cellular models (JFH1 HCV cell culture system (HCVcc) and HCV subgenomic replicons) showed that the decreased apoA-I/LDL association originates from hepatic biogenesis rather than lipoprotein catabolism occurring in the circulation, and is not due to a downregulation of the apoA-I protein concentration. The sole non-structural viral proteins were sufficient to impair the apoA-I/LDL association. Functional evidence was obtained for involvement of apoA-I in the viral life cycle such as RNA replication and virion production. The specific siRNA-mediated downregulation of apoA-I led to a reduction in both HCV RNA and viral particle levels in culture. Conclusions This study shows that HCV induces lipoprotein structural modification and that its replication and production are linked to the host lipoprotein metabolism, suggesting apoA-I as a new possible target for antiviral therapy

NK Cell Levels Correlate with Disease Activity in Patients with Multiple Sclerosis on Ocrelizumab/Rituximab Therapy

Background: Recently, research on the pathogenesis of multiple sclerosis (MS) has focused on the role of B lymphocytes and the possibility of using specific drugs, such as Ocrelizumab and Rituximab, directed toward these cells to reduce inflammation and to slow disease progression. Objective: We aimed to evaluate the effect of Ocrelizumab/Rituximab on laboratory immune parameters and identify the predictors of treatment responses. Methods: A retrospective single-center study was conducted among patients who received infusion therapy with an anti-CD20 drug to treat MS. Results: A total of 64 patients met the inclusion criteria, with 277 total cycles of therapy studied. Compared with the baseline values, anti-CD20 infusions resulted in absolute-value and percentage decreases in B lymphocyte levels and increased the absolute and percentage levels of NK cells 3 and 5 months after therapy (p < 0.001). After multivariate logistic regression analysis, a reduced percentage level of NK cells 3 months after infusion could predict disease activity 6 months after Ocrelizumab/Rituximab administration (p = 0.041). Conclusions: Lower percentage levels of NK cells 3 months after anti-CD20 infusion correlate with the presence of disease activity 6 months after therapy, confirming a possible protective role of NK cells in MS

Analisi della risposta sismica di un edificio campione nel Comune di Ariano Irpino (AV)

Nella pratica ingegneristica vengono usate correlazioni derivate da regressioni statistiche tra numero dei piani degli edifici e frequenze di risonanza. Tuttavia la discrepanza tra comportamento reale e valori aspettati può essere talvolta significativa, e solo l’acquisizione di dati sperimentali consente di comprendere il reale comportamento dinamico di una struttura. La sperimentazione, in situazioni anche complesse, e la raccolta di nuovi dati possono essere molto importanti nel campo dell’ingegneria strutturale. In questo articolo vengono presentati i risultati del monitoraggio sismico effettuato su un edificio campione in muratura (il municipio di Ariano Irpino), tipologia costruttiva largamente diffusa tra gli edifici pubblici strategici (ad esempio scuole, caserme ed ospedali). Sono state utilizzate 7 stazioni sismologiche a sei canali equipaggiate con sismometri ed accelerometri. I dati sismici sono stati acquisiti in modalità continua, in punti strategici della struttura, su diversi livelli, dal gennaio 2006 a dicembre 2007. Sono stati selezionati una ventina di terremoti di magnitudo bassa o intermedia (1.5 ≤ M ≤ 4.8) avvenuti a distanze epicentrali variabili da 4 a 116 km. Le registrazioni sono state analizzate sia mediante la tecnica dei rapporti spettrali rispetto alla base dell’edificio sia calcolando i rapporti spettrali tra componente orizzontale e verticale di ogni sensore. L’analisi svolta ha consentito di evidenziare numerose frequenze di vibrazione dell’edificio. Mediante simulazioni numeriche su un modello tridimensionale rappresentativo della struttura in esame è stato possibile associare i picchi in frequenza ai modi propri in campo lineare. In particolare, sono stati ben identificati i primi 3 modi di vibrazione (due flessionali ed uno rotazionale)