Identification of Novel Methane-, Ethane-, and Propane-Oxidizing Bacteria at Marine Hydrocarbon Seeps by Stable Isotope Probing

Marine hydrocarbon seeps supply oil and gas to microorganisms in sediments and overlying water. We used stable isotope probing (SIP) to identify aerobic bacteria oxidizing gaseous hydrocarbons in surface sediment from the Coal Oil Point seep field located offshore of Santa Barbara, California. After incubating sediment with ^(13)C-labeled methane, ethane, or propane, we confirmed the incorporation of ^(13)C into fatty acids and DNA. Terminal restriction fragment length polymorphism (T-RFLP) analysis and sequencing of the 16S rRNA and particulate methane monooxygenase (pmoA) genes in ^(13)C-DNA revealed groups of microbes not previously thought to contribute to methane, ethane, or propane oxidation. First, ^(13)C methane was primarily assimilated by Gammaproteobacteria species from the family Methylococcaceae, Gammaproteobacteria related to Methylophaga, and Betaproteobacteria from the family Methylophilaceae. Species of the latter two genera have not been previously shown to oxidize methane and may have been cross-feeding on methanol, but species of both genera were heavily labeled after just 3 days. pmoA sequences were affiliated with species of Methylococcaceae, but most were not closely related to cultured methanotrophs. Second, ^(13)C ethane was consumed by members of a novel group of Methylococcaceae. Growth with ethane as the major carbon source has not previously been observed in members of the Methylococcaceae; a highly divergent pmoA-like gene detected in the ^(13)C-labeled DNA may encode an ethane monooxygenase. Third, ^(13)C propane was consumed by members of a group of unclassified Gammaproteobacteria species not previously linked to propane oxidation. This study identifies several bacterial lineages as participants in the oxidation of gaseous hydrocarbons in marine seeps and supports the idea of an alternate function for some pmoA-like genes

A Primer On Collective Entrepreneurship: A Preliminary Taxonomy

We document an increasing prevalence of the term ―collective entrepreneurship‖ in scholarly research. By examining the context in which the term is utilized, we present a framework through which to understand motivations for research in collective entrepreneurship and the variety of entrepreneurial endeavors described as collective entrepreneurship. We identify five primary motivations for research: advancement of theory, intra-organizational efficiency, inter-organizational gains, economic growth and development, and socio-political change. We find preliminary evidence collective entrepreneurs may be able to generate rents inaccessible to the sole entrepreneur. In addition, we propose mechanisms which foster entrepreneurship may differ for sole and collective entrepreneurs.International Relations/Trade,

Lessons from Community Entrepreneurship: The Concept of Spawning

Capital-constrained cooperatives are being challenged by producer-members to provide vertical integration opportunities. We find evidence producer groups are utilizing an investment strategy described as spawning. Producer-investors familiar with a particular organizational form and who have developed joint investment networks were more apt to invest in newly spawned ventures.Spawning, Collective Entrepreneurship, Cooperative Development, Community/Rural/Urban Development,

Changes in insulin and insulin signaling in Alzheimer\u27s disease: Cause or consequence?

Individuals with type 2 diabetes have an increased risk for developing Alzheimer’s disease (AD), although the causal relationship remains poorly understood. Alterations in insulin signaling (IS) are reported in the AD brain. Moreover, oligomers/fibrils of amyloid-β (Aβ) can lead to neuronal insulin resistance and intranasal insulin is being explored as a potential therapy for AD. Conversely, elevated insulin levels (ins) are found in AD patients and high insulin has been reported to increase Aβ levels and tau phosphorylation, which could exacerbate AD pathology. Herein, we explore whether changes in ins and IS are a cause or consequence of AD

Common Mitochondrial DNA Mutations Generated through DNA-Mediated Charge Transport

Mutation sites that arise in human mitochondrial DNA as a result of oxidation by a rhodium photooxidant have been identified. HeLa cells were incubated with [Rh(phi)2bpy]Cl3 (phi is 9,10-phenanthrenequinone diimine), an intercalating photooxidant, to allow the complex to enter the cell and bind mitochondrial DNA. Photoexcitation of DNA-bound [Rh(phi)2bpy]3+ can promote the oxidation of guanine from a distance through DNA-mediated charge transport. After two rounds of photolysis and growth of cells incubated with the rhodium complex, DNA mutations in a portion of the mitochondrial genome were assessed via manual sequencing. The mutational pattern is consistent with dG to dT transversions in the repetitive guanine tracts. Significantly, the mutational pattern found overlaps oxidative damage hot spots seen previously. These mutations are found within conserved sequence block II, a critical regulatory element involved in DNA replication, and these have been identified as sites of low oxidation potential to which oxidative damage is funneled. On the basis of this mutational analysis and its correspondence to sites of long-range oxidative damage, we infer a critical role for DNA charge transport in generating these mutations and, thus, in regulating mitochondrial DNA replication under oxidative stress

Near resonance acoustic scattering from organized schools of juvenile Atlantic bluefin tuna (Thunnus thynnus)

Schools of Atlantic bluefin tuna (Thunnus thynnus) can exhibit highly organized spatial structure within the school. This structure was quantified for dome shaped schools using both aerial imagery collected from a commercial spotter plane and 400 kHz multibeam echo sounder data collected on a fishing vessel in 2009 in Cape Cod Bay, MA. Observations from one school, containing an estimated 263 fish within an approximately ellipsoidal volume of 1900 m3, were used to seed an acoustic model that estimated the school target strength at frequencies between 10 and 2000 Hz. The fish\u27s swimbladder resonance was estimated to occur at approximately 50 Hz. The acoustic model examined single and multiple scattering solutions and also a completely incoherent summation of scattering responses from the fish. Three levels of structure within the school were examined, starting with fish locations that were constrained by the school boundaries but placed according to a Poisson process, then incorporating a constraint on the distance to the nearest neighbor, and finally adding a constraint on the bearing to the nearest neighbor. Results suggest that both multiple scattering and spatial organization within the school should be considered when estimating the target strength of schools similar to the ones considered here

Pseudodeterminants and perfect square spanning tree counts

The pseudodeterminant $\textrm{pdet}(M)$ of a square matrix is the last nonzero coefficient in its characteristic polynomial; for a nonsingular matrix, this is just the determinant. If $\partial$ is a symmetric or skew-symmetric matrix then $\textrm{pdet}(\partial\partial^t)=\textrm{pdet}(\partial)^2$. Whenever $\partial$ is the $k^{th}$ boundary map of a self-dual CW-complex $X$, this linear-algebraic identity implies that the torsion-weighted generating function for cellular $k$-trees in $X$ is a perfect square. In the case that $X$ is an \emph{antipodally} self-dual CW-sphere of odd dimension, the pseudodeterminant of its $k$th cellular boundary map can be interpreted directly as a torsion-weighted generating function both for $k$-trees and for $(k-1)$-trees, complementing the analogous result for even-dimensional spheres given by the second author. The argument relies on the topological fact that any self-dual even-dimensional CW-ball can be oriented so that its middle boundary map is skew-symmetric.Comment: Final version; minor revisions. To appear in Journal of Combinatoric

Natural Time, Nowcasting and the Physics of Earthquakes: Estimation of Seismic Risk to Global Megacities

This paper describes the use of the idea of natural time to propose a new method for characterizing the seismic risk to the world's major cities at risk of earthquakes. Rather than focus on forecasting, which is the computation of probabilities of future events, we define the term seismic nowcasting, which is the computation of the current state of seismic hazard in a defined geographic region.Comment: 9 Figures, 4 Table