Introduction to the special issue "Transnational care : Families confronting borders"

In this article, we introduce the key themes of our Special Issue on “Transnational care: families confronting borders”. Central to this collection is the question of how family relations and solidarities are impacted by the current scenario of closed borders and increasingly restrictive migration regimes. This question is examined more specifically through the lens of care dynamics within transnational families and their (re-)configurations across diverse contexts marked by “immobilizing regimes of migration”. We begin by presenting a brief overview of key concepts in the transnational families and caregiving literature that provides a foundation for the diverse cases explored in the articles, including refugees and asylum seekers in Germany and Finland, Polish facing Brexit in the UK, Latin American migrants transiting through Mexico, and restrictionist drifts in migration policies in Australia, Belgium and the UK. Drawing on this rich work, we identify two policy tools; namely temporality and exclusion, which appear to be particularly salient features of immobilizing regimes of migration that significantly influence care-related mobilities. We conclude with a discussion of how immobilizing regimes are putting transnational family solidarities in crisis, including in the context of the Covid-19 pandemic, gripping the globe at the time of writing

HDAC6 mediates the acetylation of TRIM50

The E3 Ubiquitin ligase TRIM50 promotes the formation and clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction, a tubulin specific deacetylase that regulates microtubule-dependent aggresome formation. In this report we showed that TRIM50 is a target of HDAC6 with Lys-372 as a critical residue for acetylation. We identified p300 and PCAF as two TRIM50 acetyltransferases and we further showed that a balance between ubiquitination and acetylation regulates TRIM50 degradatio

Examining transnational care circulation trajectories within immobilizing regimes of migration : implications for proximate care

In this paper we argue that the current political context of restrictionist migration policies is dramatically affecting people’s capacity to cross borders to engage in proximate care with their relatives, which is a central, yet often overlooked, feature of transnational care practices. We examine how the wider context of temporality, restrictive mobility, and heightened uncertainty about the future affect people’s ability to be mobile and to move back and forth for caregiving. In examining the wellbeing effects of such restrictions, we highlight their variable impact depending on factors such as socio-economic positioning, life-course stage and health. The first sections of the paper present the care circulation framework and the particular meaning and function of proximate forms of care, as well as the main categories of care-related mobility that support this. We illustrate the main dynamics and challenges faced by transnational family members who engage in these care-related mobilities, through three vignettes involving care circulation between India and the UK, China and Australia, and Morocco and Belgium. In the final section, we discuss our vignettes in relation to the political, physical, social and time dimensions of current regimes of mobility that impact on care-related mobilities. We argue that the regimes of mobility that currently govern care-related mobilities are best understood as ‘immobilizing’ regimes with important and undervalued implications for ontological security and wellbeing

COVID-19 Accelerated Cognitive Decline in Elderly Patients with Pre-Existing Dementia Followed up in an Outpatient Memory Care Facility

Introduction: Coronavirus disease 2019 (COVID-19) may affect the cognitive function and activities of daily living (ADL) of elderly patients. This study aimed to establish the COVID-19 effect on cognitive decline and the velocity of cognitive function and ADL changes in elderly patients with dementia followed up in an outpatient memory care facility. Methods: In total, 111 consecutive patients (age 82 ± 5 years, 32% males) with a baseline visit before infection were divided into those who had or did not have COVID-19. Cognitive decline was defined as a five-point loss of Mini-Mental State Examination (MMSE) score and ADL comprising basic and instrumental ADL indexes (BADL and IADL, respectively). COVID-19 effect on cognitive decline was weighted for confounding variables by the propensity score, whereas the effect on change in the MMSE score and ADL indexes was analyzed using multivariate mixed-effect linear regression. Results: COVID-19 occurred in 31 patients and a cognitive decline in 44. Cognitive decline was about three and a half times more frequent in patients who had COVID-19 (weighted hazard ratio 3.56, 95% confidence interval 1.50–8.59, p = 0.004). The MMSE score lowered on average by 1.7 points/year, independently of COVID-19, but it lowered twice faster in those who had COVID-19 (3.3 vs. 1.7 points/year, respectively, p < 0.050). BADL and IADL indexes lowered on average less than 1 point/year, independently of COVID-19 occurrence. Patients who had COVID-19 had a higher incidence of new institutionalization than those who did not have the disease (45% versus 20%, p = 0.016, respectively). Conclusions: COVID-19 had a significant impact on cognitive decline and accelerated MMSE reduction in elderly patients with dementia

ERYTHROPOIETIN FOR THE TREATMENT OF SUBARACHNOID HEMORRAGE: A FEASIBLE INGREDIENT FOR A SUCCESS MEDICAL RECIPE

Subaracnhoid hemorrage (SAH) following aneurysm bleeding accounts for 6% to 8% of all cerebrovascular accidents. Althoug an aneurysm can be effectively managed by surgery or endovascular therapy, delayed cerebral ischemia is diagnosed in a high percentage of patients resulting in significant morbility and mortality. Cerebral vasospasm occurs in more than half of all patients after aneurysm rupture and is recognized as the leading cause of delayed cerebral ischemia after SAH. Hemodynamic strategies and endovascular procedures may be considered fo the treatment of cerebral vasospasm. In recent years, the mechanism contributing to the development of vasospasm, abnormal reactivity of cerebral arteries and cerebral ischemia following SAH, have been intensively investigated. A number of pathological processes have been identified in the pathogenesis of vasospasm including endothelial injury, smooth muscle cell contraction from spasmogenic substances produced by the subarachnoid blood clots, changes in vascular responsiveness and inflammatory response of the vascular endothelium. to date, the current therapeutic interventions remain ineffective being limited to the manipulation os systemic blood pressure, variation of blood volume and viscosity, and control of arterial carbon dioxide tension. In this scenario, the hormone erythropoietin (EPO), has been found to exert neuroprotective action during experimental SAH when its recombinant form (rHuEPO) is systematically administered. However, recent translation of experimental data into clinical trials has suggested an unclear role of recombinant human EPO in the setting of SAH. In this context, the aim of the recurrent review is to present current evidence on the potential role of EPO in cerebrovascular dysfunction following aneurysmal subarachnoid hemorrage

Warping-based co-registration of thermal infrared images: Study of factors influencing its applicability

A relevant issue for processing biomedical thermal imaging data is the availability of tools for objective and quantitative comparison of images across different conditions or subjects. To this goal, a solution can be offered by projecting the thermal distribution data onto a fictitious template to obtain a common reference for comparison across cases or subjects. In this preliminary study, we tested the feasibility of applying a warping procedure on infrared thermal images. Fifteen thermal images of checkerboard were recorded at three different distances and five different angles in order to evaluate which factor mostly influences the warping accuracy. The accuracy of three different warping transformation models (local weighted mean (LWM), polynomial, affine) was tested by comparing the positioning error between users’ selected fiduciary points on each thermal image and their corresponding reference position assigned on the template image. Fifteen users, divided into three groups upon on their experience in thermal imaging processing, participated in this study in order to evaluate the effect of experience in applying a warping procedure to the analysis of thermal infrared images. The most relevant factor influencing the positioning and thermal errors is the acquisition distance, while the users’ level of experience and the inclination angle do not seem to play the same importance. Comparing the three transformations, the LWM seems to be the best in terms of minimizing the two categories of errors. This preliminary work helps to understand the limits and the possibilities of applying warping techniques for objective, quantitative and automatic thermal image comparisons

Dosimetry for repetitive transcranial magnetic stimulation: a translational study from Alzheimer’s disease patients to controlled in vitro investigations

Objective. Recent studies have indicated that repetitive transcranial magnetic stimulation (rTMS) could enhance cognition in Alzheimer’s Disease (AD) patients, but to now the molecular-level interaction mechanisms driving this effect remain poorly understood. While cognitive scores have been the primary measure of rTMS effectiveness, employing molecular-based approaches could offer more precise treatment predictions and prognoses. To reach this goal, it is fundamental to assess the electric field (E-field) and the induced current densities (J) within the stimulated brain areas and to translate these values to in vitro systems specifically devoted in investigating molecular-based interactions of this stimulation. Approach. This paper offers a methodological procedure to guide dosimetric assessment to translate the E-field induced in humans (in a specific pilot study) into in vitro settings. Electromagnetic simulations on patients’ head models and cellular holders were conducted to characterize exposure conditions and determine necessary adjustments for in vitro replication of the same dose delivered in humans using the same stimulating coil. Main results. Our study highlighted the levels of E-field and J induced in the target brain region and showed that the computed E-field and J were different among patients that underwent the treatment, so to replicate the exposure to the in vitro system, we have to consider a range of electric quantities as reference. To match the E-field to the levels calculated in patients’ brains, an increase of at least the 25% in the coil feeding current is necessary when in vitro stimulations are performed. Conversely, to equalize current densities, modifications in the cells culture medium conductivity have to be implemented reducing it to one fifth of its value. Significance. This dosimetric assessment and subsequent experimental adjustments are essential to achieve controlled in vitro experiments to better understand rTMS effects on AD cognition. Dosimetry is a fundamental step for comparing the cognitive effects with those obtained by stimulating a cellular model at an equal dose rigorously evaluated

Involvement of Mitochondria in the Selective Response to Microsecond Pulsed Electric Fields on Healthy and Cancer Stem Cells in the Brain

In the last few years, pulsed electric fields have emerged as promising clinical tools for tumor treatments. This study highlights the distinct impact of a specific pulsed electric field protocol, PEF-5 (0.3 MV/m, 40 μs, 5 pulses), on astrocytes (NHA) and medulloblastoma (D283) and glioblastoma (U87 NS) cancer stem-like cells (CSCs). We pursued this goal by performing ultrastructural analyses corroborated by molecular/omics approaches to understand the vulnerability or resistance mechanisms triggered by PEF-5 exposure in the different cell types. Electron microscopic analyses showed that, independently of exposed cells, the main targets of PEF-5 were the cell membrane and the cytoskeleton, causing membrane filopodium-like protrusion disappearance on the cell surface, here observed for the first time, accompanied by rapid cell swelling. PEF-5 induced different modifications in cell mitochondria. A complete mitochondrial dysfunction was demonstrated in D283, while a mild or negligible perturbation was observed in mitochondria of U87 NS cells and NHAs, respectively, not sufficient to impair their cell functions. Altogether, these results suggest the possibility of using PEF-based technology as a novel strategy to target selectively mitochondria of brain CSCs, preserving healthy cells

Laser-tissue photothermal interaction: a thermal infrared imaging study

A 2-D approach, based on Infrared (IR) imaging for monitoring and optimizing the photo-therapy in dermatology, is proposed. We studied the possibility to employ IR imaging in order to select the laser treatment parameters for each patient. A Pulsed Thermography (PT) model allowed to evaluate morphological information on the specific area to treat after a single laser pulse test. The data were elaborated through a 2-D numerical simulation, which described the tissue temperature profiles for different sets of laser parameters. Based on this approach, it was possible to select the laser parameters according to specific pathology, morphology and phototype in order to achieve the best performances in the phototherapy practice