An Assessment of Relationships between Actual and Perceived Caregiving Demand and Negative Impact Measures

Previous research has focused on the time spent and the tasks involved in caregiving and on care recipient characteristics in measuring caregiving demand and predicting negative impacts (Aneshensel, Pearlin, & Schuler, 1993; McKinlay, Crawford, & Tennstedt, 1995; Stull, Bowman, & Smerglia, 1994). However, the nature of the relationship between objectively measured caregiving demand and negative impacts for caregivers remains unclear. Researchers now note that assessing caregivers\u27 perceptions of demand may be key in understanding negative impacts (Coverman, 1989; Litvin, Albert, Brody, & Hoffman, 1995; Loomis & Booth, 1995). This study assessed relationships between objective and subjective reports of caregiving demand and negative impact measures. Information about subgroups of caregivers and caregiving demand and negative impacts was also made available. The design involved a cross-sectional survey methodology that utilized means tests and correlation statistics for analysis. The findings indicate that caregiver\u27s subjective reports of demand are positively associated with negative impacts while their objective reports are inversely associated with negative impacts. The findings also show that some subgroups of caregivers experience greater negative impacts than others. Implications for practice and policy are discussed

Carboxylic ester hydrolases from hyperthermophiles

Carboxylic ester hydrolyzing enzymes constitute a large group of enzymes that are able to catalyze the hydrolysis, synthesis or transesterification of an ester bond. They can be found in all three domains of life, including the group of hyperthermophilic bacteria and archaea. Esterases from the latter group often exhibit a high intrinsic stability, which makes them of interest them for various biotechnological applications. In this review, we aim to give an overview of all characterized carboxylic ester hydrolases from hyperthermophilic microorganisms and provide details on their substrate specificity, kinetics, optimal catalytic conditions, and stability. Approaches for the discovery of new carboxylic ester hydrolases are described. Special attention is given to the currently characterized hyperthermophilic enzymes with respect to their biochemical properties, 3D structure, and classification