17 research outputs found
ΠΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ ΠΌΠ΅Π·Π΅Π½Ρ ΡΠΌΠ½ΠΈΡ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ
The content of fatty acids in the lipids of mesenchymal stem cells of dog adipose tissue culture was studied. Mesenchymal stem cells of dog adipose tissue culture were obtained by culturing the primary material in a CO2 incubator with a content of 5 % CO2, at a temperature of 37 Β°C in DMEM medium with the addition of 10β15 % fetal bovine serum and 1 % antibiotic-antimycotic. When the confluency of the monolayer reached 70β80 %, the cells were transferred to a suspension and subcultivated in order to reduce the heterogeneity of the culture and obtain a sufficient amount of biological material. The lipids of the obtained stem cells were analyzed for the content of fatty acids by the method of thin-layer gas-liquid chromatography. Determination of the content of lipids of fatty acids in FSK of a cat was carried out by the Folch method. A mixture of fatty acid methyl esters was analyzed on a Trace GC Ultra gas chromatograph with a flame ionization detector on a capillary column SPTM β2560, 100 m x 0.25 mm ID, 0.20 ΞΌm film (Supelco). Identification of fatty acids was carried out using a standard sample of Supelco 37 Π‘omponent FAME Mix. Quantitative assessment of the LC spectrum was carried out by the method of normalization of the peak planes of methylated LC derivatives and their content was determined as a percentage of the total content of all LC. The conducted study of the content of fatty acids in lipids made it possible to reveal certain features of the lipid metabolism of mesenchymal stem cells cultured in dog adipose tissue. A high content of oleic acid, characteristic of cells resistant to apoptosis and with high proliferative potential, was determined; a high ratio of unsaturated linoleic to saturated stearic acid (Π‘18:1/Π‘18.0), which reflects the high activity of the stearoyl-coenzyme-desaturase enzyme and, indirectly, the active state of the Wnt/Ξ²-catenin signaling pathway; inability to lengthen the chain of saturated fatty acids; lack or low activity of de novo synthesis of omega-6 polyunsaturated fatty acids. 18 fatty acids were found in the composition of lipids of fetal stem cells of a cat, of the saturated ones - the most palmitic acid (33.70 Β± 0.02 %), of the monounsaturated ones β oleic acid (21.63 Β± 0.03 %), of the polyunsaturated ones β linoleic acid (6.45 Β± 0.07 %). The least amount of cis-,11,14-eicosadienoic acid (0.04 Β± 0.01 %) was found in the composition of cell lipids. The total amount of saturated fatty acids in dog mesenchymal stem cell lipids was 65.65 Β± 0.02 %), unsaturated fatty acids β 34.35 Β± 0.02 %. Monoene fatty acids were determined in the amount of 24.46 Β± 0.02 %, and polyene β 9.89 Β± 0.02 %. The ratio index of polyunsaturated fatty acids Ο 3 to Ο 6 is 0.40. Lipids of mesenchymal stem cells of adipose tissue culture were characterized by a lower content of monoene unsaturated fatty acids 24.46 Β± 0.02; (P < 0.05), with a higher content of Ο3 fatty acids 3.04 Β± 0.02 %; (P < 0.05), with a lower content of Ο6 fatty acids 6.86 Β± 0.02 %; (P < 0.05) in contrast to lipids of red bone marrow stem cells.ΠΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½ΠΎ Π²ΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ
ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ. ΠΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½Ρ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²Ρ ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ ΠΎΡΡΠΈΠΌΡΠ²Π°Π»ΠΈ ΡΠ»ΡΡ
ΠΎΠΌ ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ ΠΏΠ΅ΡΠ²ΠΈΠ½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ Π² Π‘Π2 ΡΠ½ΠΊΡΠ±Π°ΡΠΎΡΡ Π· Π²ΠΌΡΡΡΠΎΠΌ 5 % Π‘Π2, Π·Π° ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠΈ 37 Β°Π‘ Ρ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΡ DMEM Π· Π΄ΠΎΠ΄Π°Π²Π°Π½Π½ΡΠΌ 10β15 % ΡΠ΅ΡΠ°Π»ΡΠ½ΠΎΡ ΡΠΈΡΠΎΠ²Π°ΡΠΊΠΈ Π²Π΅Π»ΠΈΠΊΠΎΡ ΡΠΎΠ³Π°ΡΠΎΡ Ρ
ΡΠ΄ΠΎΠ±ΠΈ ΡΠ° 1 % Π°Π½ΡΠΈΠ±ΡΠΎΡΠΈΠΊΠ°-Π°Π½ΡΠΈΠΌΡΠΊΠΎΡΠΈΠΊΠ°. ΠΠΎΠ»ΠΈ ΠΊΠΎΠ½ΡΠ»ΡΠ΅ΡΠ½ΡΡΡΡ ΠΌΠΎΠ½ΠΎΡΠ°ΡΡ ΡΡΠ³Π°Π»Π° 70β80 %, ΠΊΠ»ΡΡΠΈΠ½ΠΈ ΠΏΠ΅ΡΠ΅Π²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ΡΡΡΠΏΠ΅Π½Π·ΡΡ ΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΡΠ±ΠΊΡΠ»ΡΡΠΈΠ²ΡΠ²Π°Π½Π½Ρ Π· ΠΌΠ΅ΡΠΎΡ Π·Π½ΠΈΠΆΠ΅Π½Π½Ρ Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΠΊΡΠ»ΡΡΡΡΠΈ ΡΠ° ΠΎΡΡΠΈΠΌΠ°Π½Π½Ρ Π΄ΠΎΡΡΠ°ΡΠ½ΡΠΎΡ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΡΠ°Π»Ρ. ΠΡΠΏΡΠ΄ΠΈ ΠΎΡΡΠΈΠΌΠ°Π½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΡΠ²Π°Π»ΠΈ Π½Π° Π²ΠΌΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΎΠ½ΠΊΠΎΡΠ°ΡΠΎΠ²ΠΎΡ Π³Π°Π·ΠΎΡΡΠ΄ΠΈΠ½Π½ΠΎΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡΡ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ Π²ΠΌΡΡΡΡ Π»ΡΠΏΡΠ΄ΡΠ² ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π€Π‘Π ΠΊΠΎΡΠ° ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π€ΠΎΠ»ΡΠ°. Π‘ΡΠΌΡΡ ΠΌΠ΅ΡΠΈΠ»ΠΎΠ²ΠΈΡ
Π΅ΡΡΡΡΠ² ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π°Π½Π°Π»ΡΠ·ΡΠ²Π°Π»ΠΈ Π½Π° Π³Π°Π·ΠΎΠ²ΠΎΠΌΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡ Trace GC Ultra Π· ΠΏΠΎΠ»ΡΠΌβΡΠ½ΠΎ-ΡΠΎΠ½ΡΠ·Π°ΡΡΠΉΠ½ΠΈΠΌ Π΄Π΅ΡΠ΅ΠΊΡΠΎΡΠΎΠΌ Π½Π° ΠΊΠ°ΠΏΡΠ»ΡΡΠ½ΡΠΉ ΠΊΠΎΠ»ΠΎΠ½ΡΡ SPTMβ2560, 100 m Γ 0,25 mm ID, 0,20 ΞΌm film (Supelco). ΠΠ΄Π΅Π½ΡΠΈΡΡΠΊΠ°ΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ ΡΡΠ°Π½Π΄Π°ΡΡΠ½ΠΎΠ³ΠΎ Π·ΡΠ°Π·ΠΊΠ° Supelco 37 Π‘omponent FAME Mix. ΠΡΠ»ΡΠΊΡΡΠ½Ρ ΠΎΡΡΠ½ΠΊΡ ΡΠΏΠ΅ΠΊΡΡΡ ΠΠ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π½ΠΎΡΠΌΡΠ²Π°Π½Π½Ρ ΠΏΠ»ΠΎΡΠΈΠ½ ΠΏΡΠΊΡΠ² ΠΌΠ΅ΡΠΈΠ»ΡΠΎΠ²Π°Π½ΠΈΡ
ΠΏΠΎΡ
ΡΠ΄Π½ΠΈΡ
ΠΠ Ρ Π²ΠΈΠ·Π½Π°ΡΠ°Π»ΠΈ ΡΡ
Π½ΡΠΉ Π²ΠΌΡΡΡ Ρ Π²ΡΠ΄ΡΠΎΡΠΊΠ°Ρ
Π²ΡΠ΄ ΡΡΠΌΠ°ΡΠ½ΠΎΠ³ΠΎ Π²ΠΌΡΡΡΡ ΡΡΡΡ
ΠΠ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π²ΠΌΡΡΡΡ ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Π² Π»ΡΠΏΡΠ΄Π°Ρ
Π΄Π°Π»ΠΎ ΠΌΠΎΠΆΠ»ΠΈΠ²ΡΡΡΡ Π²ΠΈΡΠ²ΠΈΡΠΈ ΠΏΠ΅Π²Π½Ρ ΠΎΡΠΎΠ±Π»ΠΈΠ²ΠΎΡΡΡ Π»ΡΠΏΡΠ΄Π½ΠΎΠ³ΠΎ ΠΎΠ±ΠΌΡΠ½Ρ ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ ΡΠΎΠ±Π°ΠΊΠΈ. ΠΠΈΠ·Π½Π°ΡΠ΅Π½ΠΎ Π²ΠΈΡΠΎΠΊΠΈΠΉ Π²ΠΌΡΡΡ ΠΎΠ»Π΅ΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ, Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠ½ΠΈΠΉ Π΄Π»Ρ ΠΊΠ»ΡΡΠΈΠ½, ΡΠ΅Π·ΠΈΡΡΠ΅Π½ΡΠ½ΠΈΡ
Π΄ΠΎ Π°ΠΏΠΎΠΏΡΠΎΠ·Ρ ΡΠ° Π· Π²ΠΈΡΠΎΠΊΠΈΠΌ ΠΏΡΠΎΠ»ΡΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΈΠΌ ΠΏΠΎΡΠ΅Π½ΡΡΠ°Π»ΠΎΠΌ; Π²ΠΈΡΠΎΠΊΠ΅ ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½Ρ Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΎΡ Π»ΡΠ½ΠΎΠ»Π΅Π²ΠΎΡ Π΄ΠΎ Π½Π°ΡΠΈΡΠ΅Π½ΠΎΡ ΡΡΠ΅Π°ΡΠΈΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (Π‘18:1/Π‘18.0), ΡΠΊΠ΅ Π²ΡΠ΄ΠΎΠ±ΡΠ°ΠΆΠ°Ρ Π²ΠΈΡΠΎΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ° ΡΡΠ΅Π°ΡΠΎΠ»-ΠΊΠΎΠ΅Π½Π·ΠΈΠΌ-Π΄Π΅ΡΠ°ΡΡΡΠ°Π·ΠΈ ΡΠ° ΠΎΠΏΠΎΡΠ΅ΡΠ΅Π΄ΠΊΠΎΠ²Π°Π½ΠΎ β Π°ΠΊΡΠΈΠ²Π½ΠΈΠΉ ΡΡΠ°Π½ Wnt/Ξ²-ΠΊΠ°ΡΠ΅Π½ΡΠ½ ΡΠΈΠ³Π½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ»ΡΡ
Ρ; Π½Π΅Π·Π΄Π°ΡΠ½ΡΡΡΡ Π΄ΠΎ ΠΏΠΎΠ΄ΠΎΠ²ΠΆΠ΅Π½Π½Ρ Π»Π°Π½ΡΡΠ³Π° Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ; Π²ΡΠ΄ΡΡΡΠ½ΡΡΡΡ Π°Π±ΠΎ Π½ΠΈΠ·ΡΠΊΡ Π°ΠΊΡΠΈΠ²Π½ΡΡΡΡ ΡΠΈΠ½ΡΠ΅Π·Ρ de novo ΠΎΠΌΠ΅Π³Π°-6 ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ. Π£ ΡΠΊΠ»Π°Π΄Ρ Π»ΡΠΏΡΠ΄ΡΠ² ΡΠ΅ΡΠ°Π»ΡΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΠΎΡΠ° Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ 18 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ, Π· Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β Π½Π°ΠΉΠ±ΡΠ»ΡΡΠ΅ ΠΏΠ°Π»ΡΠΌΡΡΠΈΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (33,70 Β± 0,02 %), Π· ΠΌΠΎΠ½ΠΎΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β ΠΎΠ»Π΅ΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (21,63 Β± 0,03 %), Π· ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
β Π»ΡΠ½ΠΎΠ»Π΅Π²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (6,45 Β± 0,07 %). ΠΠ°ΠΉΠΌΠ΅Π½ΡΠ΅ Ρ ΡΠΊΠ»Π°Π΄Ρ Π»ΡΠΏΡΠ΄ΡΠ² ΠΊΠ»ΡΡΠΈΠ½ Π²ΠΈΡΠ²Π»Π΅Π½ΠΎ ΡΡΡ-,11,14-Π΅ΠΉΠΊΠΎΠ·Π°Π΄ΡΡΠ½ΠΎΠ²ΠΎΡ ΠΊΠΈΡΠ»ΠΎΡΠΈ (0,04 Β± 0,01 %). Π‘ΡΠΌΠ°ΡΠ½Π° ΠΊΡΠ»ΡΠΊΡΡΡΡ Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Ρ Π»ΡΠΏΡΠ΄Π°Ρ
ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΡΠΎΠ±Π°ΠΊΠΈ ΡΡΠ°Π½ΠΎΠ²ΠΈΠ»Π° 65,65Β± 0,02%), Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ β 34,35 Β± 0,02 %. ΠΠΎΠ½ΠΎΡΠ½ΠΎΠ²Ρ ΠΆΠΈΡΠ½Ρ ΠΊΠΈΡΠ»ΠΎΡΠΈ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½ΠΎ Ρ ΠΊΡΠ»ΡΠΊΠΎΡΡΡ 24,46Β± 0,02%, Π° ΠΏΠΎΠ»ΡΡΠ½ΠΎΠ²Ρ β 9,89Β± 0,02%. ΠΠ½Π΄Π΅ΠΊΡ ΡΠΏΡΠ²Π²ΡΠ΄Π½ΠΎΡΠ΅Π½Π½Ρ ΠΏΠΎΠ»ΡΠ½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ Ο 3 Π΄ΠΎ Ο 6 ΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ 0,40. ΠΡΠΏΡΠ΄ΠΈ ΠΌΠ΅Π·Π΅Π½Ρ
ΡΠΌΠ½ΠΈΡ
ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΠΆΠΈΡΠΎΠ²ΠΎΡ ΡΠΊΠ°Π½ΠΈΠ½ΠΈ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΡΠ²Π°Π»ΠΈΡΡ Π½ΠΈΠΆΡΠΈΠΌ ΡΠΌΡΡΡΠΎΠΌ ΠΌΠΎΠ½ΠΎΡΠ½ΠΎΠ²ΠΈΡ
Π½Π΅Π½Π°ΡΠΈΡΠ΅Π½ΠΈΡ
ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 24,46 Β± 0,02; (P < 0,05), Π±ΡΠ»ΡΡΠΈΠΌ Π²ΠΌΡΡΡΠΎΠΌ Ο3 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 3,04 Β± 0,02 %; (P < 0,05), ΠΌΠ΅Π½ΡΠΈΠΌ Π²ΠΌΡΡΡΠΎΠΌ Ο6 ΠΆΠΈΡΠ½ΠΈΡ
ΠΊΠΈΡΠ»ΠΎΡ 6,86 Β± 0,02 %; (P < 0,05) Π½Π° ΠΏΡΠΎΡΠΈΠ²Π°Π³Ρ Π»ΡΠΏΡΠ΄Π°ΠΌ ΡΡΠΎΠ²Π±ΡΡΠΎΠ²ΠΈΡ
ΠΊΠ»ΡΡΠΈΠ½ ΠΊΡΠ»ΡΡΡΡΠΈ ΡΠ΅ΡΠ²ΠΎΠ½ΠΎΠ³ΠΎ ΠΊΡΡΡΠΊΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠΎΠ·ΠΊΡ
ANALYSIS OF THE STRUCTURAL AND MECHANICAL PROPERTIES AND MICROMORPHOLOGICAL FEATURES OF POLYMERIC FILMS BASED ON HYDROCOLLOIDS OF VEGETABLE ORIGIN USED FOR THE PRODUCTION OF BIODEGRADABLE POLYMERS
Modern research and technology approaches to the production of biodegradable polymeric materials based on renewable resources have been reviewed. It has been found that films prepared of cellulose, chitosan, gelatin, polypeptides, casein, soy, wheat, corn, rice, and maize are being commonly used at present. The structural and mechanical properties and micromorphological features of hydrocolloids of vegetable origin promising for the production of biodegradable polymersΠ²Πβstarches, pectins, carrageenans, and agarΠ²Πβhave been studied. It has been determined that, with respect to strength and suitability for use in films of individual components, all the studied hydrocolloids can be arranged in ascending order as follows: starches, carrageenans, pectins, agar. According to analysis of the structural and mechanical properties of the films, it has been shown that the best parameters are found for the samples based on pectin P1 and agar A2. The breaking stress for these materials is 52 and 77 MPa, respectively. The breaking strain is 11.5 and 8.0%, respectively. Analysis of the micromorphology has revealed the formation of surface microdiscontinuities in the films based on high methoxyl pectins P1 and P4 and unmodified corn starch S3 and the formation of wavy folds in the case of the films of kappa-carrageenan C1; these folds are formed during drying and decrease the tensile strength of the respective films. The found features will be used in the development of technologies for the production of biodegradable polymeric materials based on hydrocolloids of vegetable origin with enhanced performance and processing characteristics
EXPRESSION OF RECOMBINANT L-PHENYLALANINE AMMONIA-LYASE IN ESCHERICHIA COLI
The pal gene coding for L-phenylalanine ammonia-lyase of Rhodosporidium toruloides (GenBank entry no. X12702.1) with optimized sequence was cloned into an expressing vector pET28a. Three parameters of expression (inductor type, duration, and temperature of induction) were optimized, which resulted in a strain producing recombinant L-phenylalanine ammonia-lyase with the maximal productivity, that is, 35 ΠΒ± 1% to total cell protein, upon utilization of 0.2% lactose (according to Studier) induction during 18 h at 37ΠΒ°C. The recombinant L-phenylalanine ammonia-lyase was found to be insoluble by 99%. Solubility of the protein did not improve upon utilization of 1 mM IPTG as an inductor instead of 0.2% lactose, or upon bacterium cultivation at various temperatures, that is 25ΠΒ°C and 37ΠΒ°C
INVESTIGATION OF METHODS OF DNA EXTRACTION FROM PLANT ORIGIN OBJECTS AND FOODS BASED ON THEM
For the last decades modern and highly efficient methods of determining the quality and safety of food products, based on the application of the latest scientific achievements were developed in the world. A special place is given to the methods based on achievements of molecular biology and genetics. At the present stage of development in the field of assessing the quality of raw materials and processed food products much attention is given to highly accurate, sensitive and specific research methods, the method of polymerase chain reaction (PCR) occupying a leading place among them. PCR is a sophisticated method that simulates the natural DNA replication and allows to detect a single specific DNA molecule in the presence of millions of other molecules. The key point in the preparation of material for PCR is the extraction of nucleic acids. The low content of DNA in plant material and the high concentration of secondary metabolites complicate the process of extraction. The key solution to this problem is highly effective method of extraction, which allows to obtain the DNA of adequate quality and purity. Comparative analysis of methods for the extraction of nucleic acids from fruit raw materials and products based on them was carried out in the study. General analysis of the experimental data allowed us to determine the most efficient method for DNA extracting. In the comparative analysis it was found out that to extract DNA from plant raw materials and food products prepared on their basis it is the most suitable to use "Sorb-GMO-A" reactants kit (set). The approach described gives us a brilliant opportunity to obtain deoxyribonucleic acid proper quality and purity
ΠΠΠΠ¦ΠΠ ΠΠΠΠΠΠ«Π Π‘ΠΠΠΠ‘Π’ΠΠ ΠΠΠΠ ΠΠΠΠΠΠ’ΠΠ ΠΠΠΠΠ‘ΠΠΠΠΠ ΠΠΠ‘ΠΠ
At the previous stages of the study, products of coconut oil hydrolysis were obtained using various enzymes of lipolytic action. It has been shown that coconut oil hydrolysates are enriched with such fatty acids as capric, lauric and stearic. The carcinogenicity of the enzymatic coconut oil hydrolysates was studied using the Ames test, using Salmonella typhimurium bacteria as a test object. The results indicate the absence of an enzymatic hydrolyzate of coconut oil carcinogenic properties.ΠΠ° ΠΏΡΠ΅Π΄ΡΠ΄ΡΡΠΈΡ
ΡΡΠ°ΠΏΠ°Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Ρ ΠΏΡΠΎΠ΄ΡΠΊΡΡ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π° ΠΊΠΎΠΊΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ»Π° Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ² Π»ΠΈΠΏΠΎΠ»ΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π΄Π΅ΠΉΡΡΠ²ΠΈΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΡ ΠΊΠΎΠΊΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ»Π° ΠΎΠ±ΠΎΠ³Π°ΡΠ΅Π½Ρ ΡΠ°ΠΊΠΈΠΌΠΈ ΠΆΠΈΡΠ½ΡΠΌΠΈ ΠΊΠΈΡΠ»ΠΎΡΠ°ΠΌΠΈ, ΠΊΠ°ΠΊ ΠΊΠ°ΠΏΡΠΈΠ½ΠΎΠ²Π°Ρ, Π»Π°ΡΡΠΈΠ½ΠΎΠ²Π°Ρ ΠΈ ΡΡΠ΅Π°ΡΠΈΠ½ΠΎΠ²Π°Ρ. ΠΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΠΎΡΡΡ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΡ
Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΠΎΠ² ΠΊΠΎΠΊΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ»Π° ΠΈΠ·ΡΡΠ°Π»ΠΈ c ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΡΠ° ΠΠΉΠΌΡΠ°, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΡΡΠ΅Π³ΠΎ Π±Π°ΠΊΡΠ΅ΡΠΈΠΈ Salmonella typhimurium Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΡΠ΅ΡΡ ΠΎΠ±ΡΠ΅ΠΊΡΠ°. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΡΡΡ ΠΎΠ± ΠΎΡΡΡΡΡΡΠ²ΠΈΠΈ Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΠ° ΠΊΠΎΠΊΠΎΡΠΎΠ²ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ»Π° ΠΊΠ°Π½ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΡΡ
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