Evaluation of a Novel Biphasic Culture Medium for Recovery of Mycobacteria: A Multi-Center Study

on L-J slants. Automated liquid culture systems are expensive. A low-cost culturing medium capable of rapidly indicating the presence of mycobacteria is needed. The aim of this study was to develop and evaluate a novel biphasic culture medium for the recovery of mycobacteria from clinical sputum specimens from suspected pulmonary tuberculosis patients.<0.001).

Synthesis and Biological Evaluation of (<i>S</i>)‑Amino-2-methyl-4‑[⁷⁶Br]bromo-3‑(<i>E</i>)‑butenoic Acid (BrVAIB) for Brain Tumor Imaging

The novel compound, (<i>S</i>)-amino-2-methyl-4-[⁷⁶Br]­bromo-3-(<i>E</i>)-butenoic acid (BrVAIB, [⁷⁶Br]<b>5</b>), was characterized against the known system A tracer, IVAIB ([¹²³I]<b>8</b>). [⁷⁶Br]<b>5</b> was prepared in a 51% ± 19% radiochemical yield with high radiochemical purity (≥98%). The biological properties of [⁷⁶Br]<b>5</b> were compared with those of [¹²³I]<b>8</b>. Results showed that [⁷⁶Br]<b>5</b> undergoes mixed amino acid transport by system A and system L transport, while [¹²³I]<b>8</b> had less uptake by system L. [⁷⁶Br]<b>5</b> demonstrated higher uptake than [¹²³I]<b>8</b> in DBT tumors 1 h after injection (3.7 ± 0.4% ID/g vs 1.5 ± 0.3% ID/g) and also showed higher uptake vs [¹²³I]<b>8</b> in normal brain. Small animal PET studies with [⁷⁶Br]<b>5</b> demonstrated good tumor visualization of intracranial DBTs up to 24 h with clearance from normal tissues. These results indicate that [⁷⁶Br]<b>5</b> is a promising PET tracer for brain tumor imaging and lead compound for a mixed system A and system L transport substrate