8 research outputs found
Evaluation of a Novel Biphasic Culture Medium for Recovery of Mycobacteria: A Multi-Center Study
on L-J slants. Automated liquid culture systems are expensive. A low-cost culturing medium capable of rapidly indicating the presence of mycobacteria is needed. The aim of this study was to develop and evaluate a novel biphasic culture medium for the recovery of mycobacteria from clinical sputum specimens from suspected pulmonary tuberculosis patients.<0.001).
Synthesis and Biological Evaluation of (<i>S</i>)‑Amino-2-methyl-4‑[<sup>76</sup>Br]bromo-3‑(<i>E</i>)‑butenoic Acid (BrVAIB) for Brain Tumor Imaging
The
novel compound, (<i>S</i>)-amino-2-methyl-4-[<sup>76</sup>Br]Âbromo-3-(<i>E</i>)-butenoic acid (BrVAIB, [<sup>76</sup>Br]<b>5</b>), was characterized against the known system
A tracer, IVAIB ([<sup>123</sup>I]<b>8</b>). [<sup>76</sup>Br]<b>5</b> was prepared in a 51% ± 19% radiochemical yield with
high radiochemical purity (≥98%). The biological properties
of [<sup>76</sup>Br]<b>5</b> were compared with those of [<sup>123</sup>I]<b>8</b>. Results showed that [<sup>76</sup>Br]<b>5</b> undergoes mixed amino acid transport by system A and system
L transport, while [<sup>123</sup>I]<b>8</b> had less uptake
by system L. [<sup>76</sup>Br]<b>5</b> demonstrated higher uptake
than [<sup>123</sup>I]<b>8</b> in DBT tumors 1 h after injection
(3.7 ± 0.4% ID/g vs 1.5 ± 0.3% ID/g) and also
showed higher uptake vs [<sup>123</sup>I]<b>8</b> in normal
brain. Small animal PET studies with [<sup>76</sup>Br]<b>5</b> demonstrated good tumor visualization of intracranial DBTs up to
24 h with clearance from normal tissues. These results indicate that
[<sup>76</sup>Br]<b>5</b> is a promising PET tracer for brain
tumor imaging and lead compound for a mixed system A and system L
transport substrate