Detection and Molecular Characterization of the SARS-CoV-2 Delta Variant and the Specific Immune Response in Companion Animals in Switzerland

In human beings, there are five reported variants of concern of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2). However, in contrast to human beings, descriptions of infections of animals with specific variants are still rare. The aim of this study is to systematically investigate SARS-CoV-2 infections in companion animals in close contact with SARS-CoV-2-positive owners (“COVID-19 households”) with a focus on the Delta variant. Samples, obtained from companion animals and their owners were analyzed using a real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) and next-generation sequencing (NGS). Animals were also tested for antibodies and neutralizing activity against SARS-CoV-2. Eleven cats and three dogs in nine COVID-19-positive households were RT-qPCR and/or serologically positive for the SARS-CoV-2 Delta variant. For seven animals, the genetic sequence could be determined. The animals were infected by one of the pangolin lineages B.1.617.2, AY.4, AY.43 and AY.129 and between zero and three single-nucleotide polymorphisms (SNPs) were detected between the viral genomes of animals and their owners, indicating within-household transmission between animal and owner and in multi-pet households also between the animals. NGS data identified SNPs that occur at a higher frequency in the viral sequences of companion animals than in viral sequences of humans, as well as SNPs, which were exclusively found in the animals investigated in the current study and not in their owners. In conclusion, our study is the first to describe the SARS-CoV-2 Delta variant transmission to animals in Switzerland and provides the first-ever description of Delta-variant pangolin lineages AY.129 and AY.4 in animals. Our results reinforce the need of a One Health approach in the monitoring of SARS-CoV-2 in animals

GRB 221009A, The BOAT

GRB 221009A has been referred to as the Brightest Of All Time (the BOAT). We investigate the veracity of this statement by comparing it with a half century of prompt gamma-ray burst observations. This burst is the brightest ever detected by the measures of peak flux and fluence. Unexpectedly, GRB 221009A has the highest isotropic-equivalent total energy ever identified, while the peak luminosity is at the $\sim99$th percentile of the known distribution. We explore how such a burst can be powered and discuss potential implications for ultra-long and high-redshift gamma-ray bursts. By geometric extrapolation of the total fluence and peak flux distributions GRB 221009A appears to be a once in 10,000 year event. Thus, while it almost certainly not the BOAT over all of cosmic history, it may be the brightest gamma-ray burst since human civilization began.Comment: Resubmitted to ApJ

GRB 160410A:The first chemical study of the interstellar medium of a short GRB

Short gamma-ray bursts (SGRBs) are produced by the coalescence of compact binary systems which are remnants of massive stars. GRB 160410A is classified as a short-duration GRB with extended emission and is currently the farthest SGRB with a redshift determined from an afterglow spectrum and also one of the brightest SGRBs to date. The fast reaction to the Neil Gehrels Swift Observatory alert allowed us to obtain a spectrum of the afterglow using the X-shooter spectrograph at the Very Large Telescope (VLT). The spectrum shows several absorption features at a redshift of z = 1.7177, in addition, we detect two intervening systems at z = 1.581 and z = 1.444. The spectrum shows Ly α in absorption with a column density of log (N(H I)/cm2) = 21.2 ± 0.2 which, together with Fe II, C II, Si II, Al II, and O I, allow us to perform the first study of chemical abundances in a SGRB host galaxy. We determine a metallicity of [X/H] = −2.3 ± 0.2 for Fe II and −2.5 ± 0.2 for Si II and no dust depletion. We also find no evidence for extinction in the afterglow spectral energy distribution modelling. The environment has a low degree of ionization and the C IV and Si IV lines are completely absent. We do not detect an underlying host galaxy down to deep limits. Additionally, we compare GRB 160410A to GRB 201221D, another high-z short GRB that shows absorption lines at z = 1.045 and an underlying massive host galaxy

GRB 160410a: The first chemical study of the interstellar medium of a short GRB

© 2023 The Author(s) Published by Oxford University Press on behalf of Royal Astronomical Society. This is the accepted manuscript version of an article which has been published in final form at https://doi.org/10.1093/mnras/stad099Short Gamma-Ray Bursts (SGRBs) are produced by the coalescence of compact binary systems which are remnants of massive stars. GRB 160410A is classified as a short-duration GRB with extended emission and is currently the farthest SGRB with a redshift determined from an afterglow spectrum and also one of the brightest SGRBs to date. The fast reaction to the Neil Gehrels Swift Observatory alert allowed us to obtain a spectrum of the afterglow using the X-shooter spectrograph at the Very Large Telescope (VLT). The spectrum shows several absorption features at a redshift of z=1.7177, in addition, we detect two intervening systems at z=1.581 and z=1.444. The spectrum shows ly-alpha in absorption with a column density of log N(HI)=21.2+/-0.2 cm$^{-2}$ which, together with FeII, CII, SiII, AlII and OI, allow us to perform the first study of chemical abundances in a SGRB host galaxy. We determine a metallicity of [X/H]=-2.3+/-0.2 for FeII and -2.5+/-0.2 for SiII and no dust depletion. We also find no evidence for extinction in the afterglow spectral energy distribution (SED) modeling. The environment has a low degree of ionisation and the CIV and SiIV lines are completely absent. We do not detect an underlying host galaxy down to deep limits. Additionally, we compare GRB 160410A to GRB 201221D, another high-z short GRB that shows absorption lines at z=1.045 and an underlying massive host galaxy.Peer reviewe

The brightest GRB ever detected: GRB 221009A as a highly luminous event at z = 0.151

Context: The extreme luminosity of gamma-ray bursts (GRBs) makes them powerful beacons for studies of the distant Universe. The most luminous bursts are typically detected at moderate/high redshift, where the volume for seeing such rare events is maximized and the star-formation activity is greater than at z = 0. For distant events, not all observations are feasible, such as at TeV energies. Aims: Here we present a spectroscopic redshift measurement for the exceptional GRB 221009A, the brightest GRB observed to date with emission extending well into the TeV regime. Methods: We used the X-shooter spectrograph at the ESO Very Large Telescope (VLT) to obtain simultaneous optical to near-IR spectroscopy of the burst afterglow 0.5 days after the explosion. Results: The spectra exhibit both absorption and emission lines from material in a host galaxy at z = 0.151. Thus GRB 221009A was a relatively nearby burst with a luminosity distance of 745 Mpc. Its host galaxy properties (star-formation rate and metallicity) are consistent with those of LGRB hosts at low redshift. This redshift measurement yields information on the energy of the burst. The inferred isotropic energy release, $E_{\rm iso} > 5 \times 10^{54}$ erg, lies at the high end of the distribution, making GRB 221009A one of the nearest and also most energetic GRBs observed to date. We estimate that such a combination (nearby as well as intrinsically bright) occurs between once every few decades to once per millennium.Comment: 9 pages, 4 figures, submitted to Astronomy & Astrophysic

The first JWST spectrum of a GRB afterglow: No bright supernova in observations of the brightest GRB of all time, GRB 221009A

We present JWST and Hubble Space Telescope (HST) observations of the afterglow of GRB\,221009A, the brightest gamma-ray burst (GRB) ever observed. Observations obtained with NIRSPEC (0.6-5.5 micron) and MIRI (5-12 micron) 12 days after the burst are the first mid-IR spectroscopy performed for a GRB. Assuming the underlying slope is that of a single power-law, we obtain $\beta \approx 0.35$ and $A_V = 4.9$, in excess of the notional Galactic value. This is suggestive of extinction above the notional Galactic value, possibly due to patchy extinction within the Milky Way or dust in the GRB host galaxy. It further implies that the X-ray and optical/IR regimes are not on the same branch of the synchrotron spectrum of the afterglow. If the cooling break lies between the X-ray and optical/IR, then the temporal declines would only match for a post jet break, ISM medium and electron index with $p<2$. The shape of the JWST spectrum is near-identical in the optical/nIR to X-shooter spectroscopy obtained at 0.5 days and to later time observations with HST. The lack of spectral evolution suggests the SNe is either substantially fainter or bluer than SN~1998bw. Our {\em HST} observations also reveal a disc-like host galaxy, viewed close to edge-on that further complicates the isolation of any supernova component. The host galaxy appears rather typical amongst long-GRB hosts and suggests that the extreme properties of GRB 221009A are not directly tied to its galaxy-scale environment.Comment: Submitted to the Astrophysical Journal Letters for the GRB 221009A Special Issue. The results of this paper are under press embargo. 18 pages, 8 figures, 2 table

Carbon Nanotubes in Tissue Engineering

For their peculiar features carbon nanotubes (CNTs) are emerging in many areas of nanotechnology applications. CNT-based technology has been increasingly proposed for biomedical applications, to develop biomolecule nanocarriers, bionanosensors and smart material for tissue engineering purposes. In the following chapter this latter application will be explored, describing why CNTs can be considered an ideal material able to support and boost the growth and the proliferation of many kind of tissues

Review: The increasing importance of carbon nanotubes and nanostructured conducting polymers in biosensors

The growing need for analytical devices requiring smaller sample volumes, decreased power consumption and improved performance have been driving forces behind the rapid growth in nanomaterials research. Due to their dimensions, nanostructured materials display unique properties not traditionally observed in bulk materials. Characteristics such as increased surface area along with enhanced electrical/optical properties make them suitable for numerous applications such as nanoelectronics, photovoltaics and chemical/biological sensing. In this review we examine the potential that exists to use nanostructured materials for biosensor devices. By incorporating nanomaterials, it is possible to achieve enhanced sensitivity, improved response time and smaller size. Here we report some of the success that has been achieved in this area. Many nanoparticle and nanofibre geometries are particularly relevant, but in this paper we specifically focus on organic nanostructures, reviewing conducting polymer nanostructures and carbon nanotubes

Multi-band analyses of the bright GRB~230812B and the associated SN2023pel

GRB~230812B is a bright and relatively nearby ($z =0.36$) long gamma-ray burst that has generated significant interest in the community and therefore has been subsequently observed over the entire electromagnetic spectrum. We report over 80 observations in X-ray, ultraviolet, optical, infrared, and sub-millimeter bands from the GRANDMA (Global Rapid Advanced Network for Multi-messenger Addicts) network of observatories and from observational partners. Adding complementary data from the literature, we then derive essential physical parameters associated with the ejecta and external properties (i.e. the geometry and environment) and compare with other analyses of this event (e.g. Srinivasaragavan et al. 2023). We spectroscopically confirm the presence of an associated supernova, SN2023pel, and we derive a photospheric expansion velocity of v $\sim$ 17$\times10^3$ km $s^{-1}$. We analyze the photometric data first using empirical fits of the flux and then with full Bayesian Inference. We again strongly establish the presence of a supernova in the data, with an absolute peak r-band magnitude $M_r = - 19.41 \pm 0.10$. We find a flux-stretching factor or relative brightness $k_{\rm SN}=1.04 \pm 0.09$ and a time-stretching factor $s_{\rm SN}=0.68 \pm 0.05$, both compared to SN1998bw. Therefore, GRB 230812B appears to have a clear long GRB-supernova association, as expected in the standard collapsar model. However, as sometimes found in the afterglow modelling of such long GRBs, our best fit model favours a very low density environment ($\log_{10}({n_{\rm ISM}/{\rm cm}^{-3}}) = -2.16^{+1.21}_{-1.30}$). We also find small values for the jet's core angle $\theta_{\rm core}={1.70^{+1.00}_{-0.71}} \ \rm{deg}$ and viewing angle. GRB 230812B/SN2023pel is one of the best characterized afterglows with a distinctive supernova bump