Angiostrongylus costaricensis (Nematoda: Protostrongylidae): estudo da via migratória em Omalonyx matheroni (Gastropoda: Succineidae) e análise comparativa da resposta hemocitária e desenvolvimento larvar em moluscos pulmonados

Submitted by Nuzia Santos ([email protected]) on 2017-08-25T16:41:40Z No. of bitstreams: 1 Lângia Colli Montresor.pdf: 4417728 bytes, checksum: a250aa0cc58e1918795bf20f33bb7b2a (MD5)Approved for entry into archive by Nuzia Santos ([email protected]) on 2017-08-25T16:47:30Z (GMT) No. of bitstreams: 1 Lângia Colli Montresor.pdf: 4417728 bytes, checksum: a250aa0cc58e1918795bf20f33bb7b2a (MD5)Made available in DSpace on 2017-08-25T16:47:30Z (GMT). No. of bitstreams: 1 Lângia Colli Montresor.pdf: 4417728 bytes, checksum: a250aa0cc58e1918795bf20f33bb7b2a (MD5) Previous issue date: 2005Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.O nematódeo Angiostrongylus costaricensis tem como hospedeiros intermediários diversos moluscos pulmonados, no entanto, sua via migratória foi estudada somente em Sarasinula marginata . Neste trabalho investigou se 1 a via migratória do parasita em Omalonyx matheroni , 2 a reação tecidual e desenvolvimento larvar em alguns pulmonados ( O. matheroni, Biomphalaria glabrata, Deroceras sp. e S. marginata ), e 3 o potencial como hospedeiro intermediário de O. matheroni e Deroceras sp.. Espécimes de O. matheroni foram infectados com 1200 larvas L1 para preparação de co rtes histológicos corados em HE. Para a via migratória foram estudados pontos com 30 minuto s, 1, 2, 4, 6 e 8 horas, 2, 5, 10, 12, 15, 20, 21, 25, 28 e 30 dias de infecção. A análise dos cortes histológicos revelou a ocorrência de infecção oral e cutânea. A via migratória mostrou s e intimamente relacionada às vias do sistema circulatório indicando que as larvas são po r estas conduzidas aos diversos órgãos. No estudo comparativo da reação hemocitária nos pulmon ados esta diferiu em intensidade e grau de ativação celular. Todas as espécies se tornaram mais responsivas a L2 e à face côncava larvar (ventral). Deroceras sp. e O. matheroni apresentaram uma camada colágena ao redor dos granulomas, ausente em S. marginata e B. glabrata . A análise comparativa da resposta hemocitária em gastrópodes pulmonados demonstrou qu e os aspectos da infecção são muito semelhantes entre as espécies estudadas. No entanto , muitos padrões se mostraram variáveis (intensidade da resposta, ativação celular – morfol ogia – e presença de colágeno), demonstrando o alto grau de conservação do sistema imunológico inato e a heterogeneidade funcional dos hemócitos. No estudo do desenvolvimen to larvar 10 exemplares de cada espécie foram infectados (400L1) e digeridos após 5, 10, 15 , 20 e 30 dias. A porcentagem de cada estádio larvar recuperado com 15, 20 e 30 dias, apr esentou diferenças significativas (qui quadrado) entre as espécies. As larvas L3 obtidas d e O. matheroni e Deroceras sp. foram utilizadas para infecção oral de camundongos que, a pós 30 dias, apresentaram vermes adultos no plexo mesentérico, constatando a infectividade d as larvas e o potencial destas espécies como hospedeiras intermediárias.Different species of mollusks are intermediate host s of the nematode Angiostrongylus costaricensis . This work aimed to investigate 1 the migratory r oute of the nematode in Omalonyx matheroni , 2 the hemocytic perilarval reaction and larval d evelopment in pulmonate snails ( O. matheroni, Biomphalaria glabrata, Deroceras sp. e S. marginata ) and 3 the potential of O. matheroni and Deroceras sp. as intermediate hosts. For histological studie s O. matheroni specimens were infected with 1200 L1 larvae. The m igratory route was described by analyzing hematoxilin eosin stained sl ides from formalin millonig fixed specimens at 30 minutes, 1, 2, 4, 6 and 8 hours, 2, 5, 10, 12, 15, 20, 21, 25, 28 and 30 days after infection. Larval penetration through the ski n and digestive tract were observed for all studied species. The migratory route showed to be h ighly related to circulatory system, which provides the access ways to many organs and regions of snail’s body. The comparative study of hemocytic reaction in pulmonate gastropods showe d that although there are many similarities related to the aspects of infection, s ome patterns are variant (response intensity, cellular activation –morphology and presence of c ollagen), demonstrating conserved innate immune mechanisms and some functional heterogeneity in mollusk’s hemocytes. To follow the larval development, groups of 10 individuals of eac h species were infected (400 L1) and digested after 5, 10, 15, 20, 30 days. We found sig nificant inter specific differences between the percentages of larval stages recovered 15, 20 a nd 30 days after infection. Third stage larvae recovered from O. matheroni and Deroceras sp. were used to infect Mus musculus . The presence of adult worms in mesenteric vessels after 30 days of infection demonstrates the potential of both species as intermediate hosts

Freshwater gastropods of the Baixada Maranhense Microregion, an endemic area for schistosomiasis in the State of Maranhão, Brazil: I - qualitative study

Introduction: The Baixada Maranhense Microregion currently has the highest prevalence of schistosomiasis in the State of Maranhão, likely because this parasitosis is characterized as an occupational disease, and increased contact with water increases the risk of infection by Schistosoma mansoni. This paper reports the results of the first comprehensive freshwater malacological survey performed in the Baixada Maranhense Microregion. Methods: Freshwater mollusks were collected from the twenty-one municipalities of the Baixada Maranhense Microregion and from Bacurituba and Cajapió and were evaluated for infection by trematodes. Results: A total of 9,129 mollusks were collected (sixteen species), which included the first records of six species in the State of Maranhão: Gundlachia radiata, G. ticaga, Hebetancylus moricandi, Plesiophysa guadeloupensis, Pomacea bridgesii diffusa and Omalonyx sp. Biomphalaria glabrata was found in five municipalities, whereas B. straminea was found in nine. Biomphalaria glabrata and B. straminea were observed in syntopy in Pinheiro and São Bento. Of the 990 specimens of B. glabrata and the 2,109 specimens of B. straminea that were exposed to and/or analyzed for the presence of larval trematodes, only a single specimen of B. glabrata (0.1%) from São Bento shed S. mansoni. Other larval trematodes were first observed in mollusks from the State of Maranhão. Conclusions: These results indicate that the study area is epidemiologically important due to the presence of two natural vectors of schistosomiasis and the active transmission of schistosomiasis, which was confirmed in the infected specimen that was collected in this study

Laminosioptes cysticola in free-range chickens in Minas Gerais, Brazil Laminosioptes cysticola em galinhas de fundo de quintal em Minas Gerais, Brasil

The subcutaneous acarid parasite Laminosioptes cysticola, the fowl cyst mite, has been found for a number of years in free-range chickens of peri-urban and rural Minas Gerais, forming collagen cysts which may be subsequently calcified after the parasite's death. The role of free-range chickens as hosts and the possible risk of food-borne human allergy, as a result of its consumption in feed, are considered.<br>O parasito acarino subcutâneo Laminosioptes cysticola tem sido encontrado em galinhas de subsistência periurbanas e rurais em Minas Gerais, formando cistos arredondados que podem calcificar sob a pele após a morte do parasito. Detalhes do parasitismo e do parasito são discutidos, sendo especulado o risco potencial humano em alergia de origem alimentar pelo consumo da carne da ave contendo os cistos