1 research outputs found
Magnetic nanoparticles--templated assembly of protein subunits: a new platform for carbohydrate-based MRI nanoprobes.
A new approach for the preparation of carbohydrate-coated magnetic nanoparticles
is reported. In a first step, we show that the pH-driven assembly-disassembly
natural process that occurs in apoferritin protein is effective for the
encapsulation of maghemite nanoparticles of different sizes: 4 and 6 nm. In a
second step, we demonstrate that the presence of functional amine groups in the
outer shell of apoferritin allows functionalization with two carbohydrates,
N-acetyl-D-glucosamine and d-mannose. High-resolution electron microscopy (HREM),
high angle annular dark field scanning electron microscopy (HAADF-STEM), electron
energy loss spectroscopy (EELS), X-ray diffraction (XRD), and SQUID technique
have been used to characterize the magnetic samples, termed herein Apomaghemites.
The in vivo magnetic resonance imaging (MRI) studies showed the efficiency in
contrasting images for these samples; that is, the r(2) NMR relaxivities are
comparable with Endorem (a commercial superparamagnetic MRI contrast agent). The
r(2) relaxivity values as well as the pre-contrast and post-contrast
T(2)*-weighted images suggested that our systems could be used as perspective
superparamagnetic contrast agents for magnetic resonance imaging (MRI). The
carbohydrate-functionalized Apomaghemite nanoparticles retained their recognition
abilities, as demonstrated by the strong affinity with their corresponding
carbohydrate-binding lectins