High-throughput peptide quantification using mTRAQ reagent triplex

<p>Abstract</p> <p>Background</p> <p>Protein quantification is an essential step in many proteomics experiments. A number of labeling approaches have been proposed and adopted in mass spectrometry (MS) based relative quantification. The mTRAQ, one of the stable isotope labeling methods, is amine-specific and available in triplex format, so that the sample throughput could be doubled when compared with duplex reagents.</p> <p>Methods and results</p> <p>Here we propose a novel data analysis algorithm for peptide quantification in triplex mTRAQ experiments. It improved the accuracy of quantification in two features. First, it identified and separated triplex isotopic clusters of a peptide in each full MS scan. We designed a schematic model of triplex overlapping isotopic clusters, and separated triplex isotopic clusters by solving cubic equations, which are deduced from the schematic model. Second, it automatically determined the elution areas of peptides. Some peptides have similar atomic masses and elution times, so their elution areas can have overlaps. Our algorithm successfully identified the overlaps and found accurate elution areas. We validated our algorithm using standard protein mixture experiments.</p> <p>Conclusions</p> <p>We showed that our algorithm was able to accurately quantify peptides in triplex mTRAQ experiments. Its software implementation is compatible with Trans-Proteomic Pipeline (TPP), and thus enables high-throughput analysis of proteomics data.</p

Reinvestigation of aminoacyl-TRNA synthetase core complex by affinity purification-mass spectrometry reveals TARSL2 as a potential member of the complex

10.1371/journal.pone.0081734PLoS ONE812-POLN

유연 전자소자 구현을 위한 고 해상도의 은 나노와이어 미세패턴 인쇄 기술

prohibitionⅠ. Introduction 1 1.1 Silver nanowire (AgNW) transparent conductive electrodes 2 1.2 Micro patterning technologies 2 1.2.1 Inkjet printing 2 1.2.2 Screen printing 2 1.2.3 Gravure printing 2 1.2.4 Reverse offset printing 3 Ⅱ. Experimental 5 2.1 Preparation of AgNW ink 5 2.2 Reverse offset printing of AgNW ink 5 2.3 IPL irradiation and chemical treatments 5 2.4 Characterization of AgNW micro-patterns 6 2.5 Fabrication of flexible OLEDs and heater 6 Ⅲ. Result and discussions 7 3.1 AgNW micro-pattern fabrication using reverse offset printing 7 3.2 AgNW ink development for reverse offset printing 9 3.3 Reverse offset printing parameter optimization 11 3.4 Reverse offset printed AgNW micro-patterns 14 3.5 IPL irradiation of AgNW micro-patterns 18 3.6 Electrical & mechanical properties of AgNW micro-patterns 21 3.7 Applications 25 Ⅳ. Conclusion 29 References 30MASTERdCollectio

Silver fractal dendrites for highly sensitive and transparent polymer thermistors

Effective temperature measurement using non-invasive sensors finds applications in virtually every field of human life. Recently, significant efforts have been made toward developing polymer positive temperature coefficient (PTC) thermistors because they have advantages including flexibility, conformability, and biocompatibility. However, most polymer PTC thermistors still have issues such as low sensitivity, low optical transparency, and poor operational durability because of low electrical conductivity and inefficient hopping transport of conventional conductive filler. Here, a highly sensitive and transparent polymer thermistor composed of silver fractal dendrites (AgFDs) and a polyacrylate (PA) matrix has been successfully demonstrated. A AgFDs-PA composite film exhibits a superior PTC effect (about 104 Ω °C-1) around 35 °C because of the high electrical conductivity of the AgFDs and the quantum tunneling effect among them. A thermistor based on the AgFDs-PA composite shows excellent sensitivity, PTC intensity (∼107), and sensing resolution through dramatic resistance changes from thousands to billions of ohms in the human body temperature range (34-37 °C). Moreover, it exhibits excellent optical transparency (82.14%), mechanical flexibility, and operational durability. An electrical impedance spectroscopy analysis shows that the distance between the AgFDs increases with temperature, which implies that the quantum tunneling effect amplified by the branches of the AgFDs has a significant influence on the changes in resistance. This characteristic makes the thermistor immediately suitable for monitoring body temperature. We anticipate that the new thermistor based on the AgFDs-PA composite can be a key component of various sensing applications. © The Royal Society of Chemistry.1

High-performance transparent pressure sensors based on sea-urchin shaped metal nanoparticles and polyurethane microdome arrays for real-time monitoring

An ultra-sensitive and transparent piezoresistive pressure sensor based on a sea-urchin shaped metal nanoparticle (SSNP)-polyurethane (PU) composite with microdome arrays is successfully fabricated for the first time. The piezoresistive pressure sensor with microdome arrays was prepared using a nanoimprinting process based on an intermediate polymer substrate (IPS) replica mold. It showed a superior sensitivity (71.37 kPa-1) and a high optical transmittance (77.7% at 550 nm) due to the effective quantum tunneling effect even at small concentrations of conductive SSNP filler (6 mg mL-1). The high-performance characteristics of the piezoresistive pressure sensor are attributed to the geometric effects of the microdome structure, especially the stress concentration at small contact spots and the deformation of the contact area. The piezoresistive pressure sensor with microdome arrays also exhibited a fast response/relaxation time (30 ms), ultra-low pressure detection (4 Pa), and excellent long-term stability under harsh conditions. In addition, the effectiveness of the piezoresistive pressure sensors in various sensing applications including sensing mapping, human arterial pulse monitoring, and the detection of muscle movement is also successfully demonstrated. It is anticipated that this novel transparent pressure sensor based on a SSNP-PU composite with microdome arrays will be a key component in the development of integrated transparent sensing applications. © 2018 The Royal Society of Chemistry.1

A 122-mW Low-Power Multiresolution Spectrum-Sensing IC With Self-Deactivated Partial Swing Techniques

A low-power multiresolution spectrum-sensing (LP-MRSS) IC utilizing self-deactivated partial swing techniques is fabricated in 0.18-??m complementary metaloxideCMOS technology. The LP-MRSS is composed of a low-power digital window generator, analog correlators, low-power pipeline analog-to-digital converters, and a fast-sweeping frequency synthesizer. The LP-MRSS dissipates 122 mW at a 1.8-V supply voltage achieving an approximately 33% power reduction over the previous MRSS IC.close91

High-Resolution and Large-Area Patterning of Highly Conductive Silver Nanowire Electrodes by Reverse Offset Printing and Intense Pulsed Light Irradiation

Conventional printing technologies such as inkjet, screen, and gravure printing have been used to fabricate patterns of silver nanowire (AgNW) transparent conducting electrodes (TCEs) for a variety of electronic devices. However, they have critical limitations in achieving micrometer-scale fine line width, uniform thickness, sharp line edge, and pattering of various shapes. Moreover, the optical and electrical properties of printed AgNW patterns do not satisfy the performance required by flexible integrated electronic devices. Here, we report a high-resolution and large-area patterning of highly conductive AgNW TCEs by reverse offset printing and intense pulsed light (IPL) irradiation for flexible integrated electronic devices. A conductive AgNW ink for reverse offset printing is prepared by carefully adjusting the composition of AgNW content, solvents, surface energy modifiers, and organic binders for the first time. High-quality and high-resolution AgNW micropatterns with various shapes and line widths are successfully achieved on a large-area plastic substrate (120 × 100 mm 2 ) by optimizing the process parameters of reverse offset printing. The reverse offset printed AgNW micropatterns exhibit superior fine line widths (up to 6 μm) and excellent pattern quality such as sharp line edge, fine line spacing, effective wire junction connection, and smooth film roughness. They are post-processed with IPL irradiation, thereby realizing excellent optical, electrical, and mechanical properties. Furthermore, flexible OLEDs and heaters based on reverse offset printed AgNW micropatterns are successfully fabricated and characterized, demonstrating the potential use of the reverse offset printing for the conductive AgNW ink. © 2019 American Chemical Society.FALS

Low-Power Technique for SRAM-Based On-Chip Arbitrary-Waveform Generator

A low-power technique for a static random-access memory (SRAM)-based on-chip arbitrary-waveform generator (AWG) is proposed for two types of analog-signal-processing applications: multiresolution spectrum sensing and matched filter. The SRAM has an embedded address generator to limit the operation in a sequential-access mode of the AWG. Then, the power consumption of the AWG is analyzed according to the operation modes in multiresolution and multiwaveform spectrum-sensing functions. The low-power technique reduces power by 18% of the SRAM and the address generator and by about 2.2% of the entire AWG at a 1.8-V supply voltage. The AWG is fabricated in a 0.18-mu m CMOS technology and demonstrates a chirp signal and a Daubechies wavelet with a 45-dBc spurious-free dynamic range and a cross-correlation factor of 0.96-0.988 with ideal signals.close0