Necrotizing fasciitis involving the chest and abdominal wall caused by Raoultella planticola

<p>Abstract</p> <p>Background</p> <p><it>Raoultella planticola </it>was originally considered to be a member of environmental <it>Klebsiella</it>. The clinical significance of <it>R. planticola </it>is still not well known.</p> <p>Case presentation</p> <p>We describe the first case of necrotizing fasciitis involving the chest and abdominal wall caused by <it>R. planticola</it>. The identity of the organism was confirmed using 16S rRNA sequencing. The patient was successfully treated with the appropriate antibiotics combined with operative drainage and debridement.</p> <p>Conclusions</p> <p><it>R. planticola </it>had been described as environmental species, but should be suspected in extensive necrotizing fasciitis after minor trauma in mild to moderate immunocompromised patients.</p

Fucoidan from Marine Brown Algae Inhibits Lipid Accumulation

In this study, we elucidated the inhibitory effect of fucoidan from marine brown algae on the lipid accumulation in differentiated 3T3-L1 adipocytes and its mechanism. The treatment of fucoidan in a dose-dependent manner was examined on lipid inhibition in 3T3-L1 cells by using Oil Red O staining. Fucoidan showed high lipid inhibition activity at 200 μg/mL concentration (P < 0.001). Lipolytic activity in adipocytes is highly dependent on hormone sensitive lipase (HSL), which is one of the most important targets of lipolytic regulation. Here, we examined the biological response of fucoidan on the protein level of lipolysis pathway. The expressed protein levels of total hormone sensitive lipase (HSL) and its activated form, phosphorylated-HSL were significantly increased at concentration of 200 μg/mL fucoidan. Furthermore, insulin-induced 2-deoxy-d-[3H] glucose uptake was decreased up to 51% in fucoidan-treated cells as compared to control. Since increase of HSL and p-HSL expression and decrease of glucose uptake into adipocytes are known to lead to stimulation of lipolysis, our results suggest that fucoidan reduces lipid accumulation by stimulating lipolysis. Therefore, these results suggest that fucoidan can be useful for the prevention or treatment of obesity due to its stimulatory lipolysis

Alishewanella aestuarii sp. nov., isolated from tidal flat sediment, and emended description of the genus Alishewanella

The genus Alishewanella, proposed by Fonnesbech Strain B11 T was isolated from tidal flat sediment in Yeosu (34 u 479 260 N 127 u 349 010 E), Republic of Korea. The strain was isolated on R2A agar (Difco) followed by repeated restreaking to obtain a pure culture. The Gram reaction was determined according to the non-staining method described by T and closely related strains. Chromosomal DNA was extracted and purified using a DNA extraction kit (IntronBiotechnology). The G+C content was determined using HPLC as described by T is affiliated phylogenetically with A. fetalis; this affiliation is supported by high bootstrap values (100, 98 and 94 % by the neighbour-joining, maximum-parsimony and maximum-likelihood methods, respectively). Similarities between the 16S rRNA gene sequence of strain B11 T and those of A. fetalis CCUG 30811 T Rheinheimera aquimaris SW-353 T and Rheinheimera perlucida BA131 T were 98.3, 96.5 and 96.4 %, respectively. A DNA-DNA hybridization experiment was performed as described previously For quantitative analysis of cellular fatty acids, strain B11 T and A. fetalis CCUG 30811 T were grown under the same conditions on blood agar plates at 30 u C for 2 days. Cells were harvested and cellular fatty acids were saponified, methylated and extracted as described by the Sherlock Microbial Identification System T were C 18 : 1 v7c, C 17 : 1 v8c, summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH), C 16 : 0 and C 17 : 0 . Complete fatty acid compositions of the novel strain and A. fetalis CCUG 30811 T are shown in T and the type strain of A. fetalis are similar. In addition to 16S rRNA gene similarity, the major fatty acid components of strain B11 T and its temperature range for growth confirm affiliation of this strain with the genus Alishewanella

Multiple human papillomavirus infection is associated with high-risk infection in male genital warts in Ulsan, Korea

Further understanding of male human papillomavirus (HPV) infection is necessary to prevent infection in men, as well as transmission to women. In our current study, we investigated patterns of HPV infection and genotype distributions in male genital warts using the Anyplex II HPV28 Detection kit. We reviewed the medical records of 80 male patients who presented to 5 neighborhood clinics in Ulsan, Korea, for the treatment of genital warts between April 2014 and January 2015. All patients underwent HPV genotyping. The prevalence and characteristics of HPV infection were analyzed, and the patterns of HPV infection according to age were assessed. Among the study patients, 13 (16.3%) were negative for HPV infection, 46 (57.3%) were infected with low-risk HPV, and 21 (26.3%) were infected with high-risk HPV. Patients with multiple HPV infection were more likely to have high-risk HPV infection (P = 0.001). The prevalence of HPV infection was much higher in samples obtained by tissue excision due to a definite lesion (P = 0.001). There were no differences in high-risk HPV infection (P = 0.459), multiple HPV infection (P = 0.185), and recurrence at diagnosis (P = 0.178) according to age. HPV-6 and HPV-11 were the most common type overall (39.7% and 13.8%, respectively). HPV- 16 and HPV-18 were the most common high-risk infections (both 3.4%). HPV infection is not only commonly encountered in male genital warts, but is also accompanied by highrisk HPV and multiple infections. &amp;#169; 2016 The Korean Academy of Medical Sciencesopen

IS6110-Restriction Fragment Length Polymorphism and Spoligotyping Analysis of Mycobacterium tuberculosis Clinical Isolates for Investigating Epidemiologic Distribution in Korea

The Beijing family of Mycobacterium tuberculosis has been emerging in the world. However, there are few nationwide data of genotypic distribution in Korea. This study aimed to identify the genotypic diversity of clinical isolates of M. tuberculosis and to demonstrate the population of Beijing family in Korea. We collected 96 clinical M. tuberculosis isolates from 11 university hospitals nationwide in Korea from 2008 to 2009. We observed 24 clusters in IS6110-RFLP analysis and 19 patterns in spoligotyping. Seventy-five isolates were confirmed to be Beijing family. Two isolates of the K strain and 12 isolates of the K family strain were also found. We found that drug resistance phenotypes were more strongly associated with Beijing family than non-Beijing family (P=0.003). This study gives an overview of the distribution of genotypes of M. tuberculosis in Korea. These findings indicate that we have to pay more attention to control of M. tuberculosis strains associated with the Beijing family

Performance Comparison of KOMPSAT-5 Precision Orbit Determination with GRACE

The Korean Multipurpose Satellite-5 (KOMPSAT-5) launched on 22 August 2013 was equipped with a global positioning system (GPS) receiver for precision orbit determination (POD). Even though the GPS receiver of KOMPSAT-5 shares the same heritage as the BlackJack receiver onboard in Gravity Recovery and Climate Experiment (GRACE) satellites, KOMPSAT-5 has a lower orbital position accuracy (~10 cm) compared with GRACE (~2 cm). The reduced dynamic and kinematic methods are applied for POD of KOMPSAT-5 and GRACE to investigate the GPS observation quality due to the satellite operation concept and hardware design. The results are analyzed in terms of the number of observations and their spatial distribution, GPS signal quality, and orbital position accuracies. The results show that the frequent attitude maneuvers of KOMPSAT-5 affect the quality of the GPS signals and solutions obtained from the kinematic method compared with that determined from the reduced-dynamic method. The onboard patch GPS antenna installed in KOMPSAT-5 and its geometrical position resulted in more erratic measurement residuals by 140% compared with the choke ring antenna of GRACE. The POD accuracy is dependent on the hardware design and regular attitude tilting for the synthetic aperture radar (SAR) imaging even though the same GPS receiver performances

The Effects of the IERS Conventions (2010) on High Precision Orbit Propagation

The Earth is not perfectly spherical and its rotational axis is not fixed in space, and these geophysical and kinematic irregularities work as dominant perturbations in satellite orbit propagation. The International Earth Rotation Service (IERS) provides the Conventions as guidelines for using the Earth’s model and the reference time and coordinate systems defined by the International Astronomical Union (IAU). These guidelines are directly applied to model orbital dynamics of Earth satellites. In the present work, the effects of the latest conventions released in 2010 on orbit propagation are investigated by comparison with cases of applying the previous guidelines, IERS Conventions (2003). All seven major updates are tested, i.e., for the models of the precession/nutation, the geopotential, the ocean tides, the ocean pole tides, the free core nutation, the polar motion, and the solar system ephemeris. The resultant position differences for one week of orbit propagation range from tens of meters for the geopotential model change from EGM96 to EGM2008 to a few mm for the precession/nutation model change from IAU2000 to IAU2006. The along-track differences vary secularly while the cross-track components show periodic variation. However, the radial-track position differences are very small compared with the other components in all cases. These phenomena reflect the variation of the ascending node and the argument of latitude. The reason is that the changed models tested in the current study can be regarded as small fluctuations of the geopotential model from the point of view of orbital dynamics. The ascending node and the argument of latitude are more sensitive to the geopotential than the other elements. This study contributes to understanding of the relation between the Earth’s geophysical properties and orbital motion of satellites as well as satellite-based observations

Chandler Wobble and Free Core Nutation: Theory and Features

Being a torque free motion of the rotating Earth, Chandler wobble is the major component in the Earth’s polar motion with amplitude about 0.05-0.2 arcsec and period about 430-435 days. Free core nutation, also called nearly diurnal free wobble, exists due to the elliptical core-mantle boundary in the Earth and takes almost the whole part of un-modelled variation of the Earth’s pole in the celestial sphere beside precession and nutation. We hereby present a brief summary of their theories and report their recent features acquired from updated datasets (EOP C04 and ECMWF) by using Fourier transform, modelling, and wavelet analysis. Our new findings include (1) period-instability of free core nutation between 420 and 450 days as well as its large amplitude-variation, (2) re-determined Chandler period and its quality factor, (3) fast decrease in Chandler amplitude after 2010

Radiosonde Sensors Bias in Precipitable Water Vapor From Comparisons With Global Positioning System Measurements

In this study, we compared the precipitable water vapor (PWV) data derived from the radiosonde observation data at Sokcho Observatory and the PWV data at Sokcho Global Positioning System (GPS) Observatory provided by Korea Astronomy and Space Science Institute, for the years of 2006, 2008, 2010, and analyzed the radiosonde seasonal, diurnal bias according to radiosonde sensor types. In the scatter diagram of the daytime and nighttime radiosonde PWV data and the GPS PWV data, dry bias was found in the daytime radiosonde observation as known in the previous study. Overall, the tendency that the wet bias of the radiosonde PWV increased as the GPS PWV decreased and the dry bias of the radiosonde PWV increased as the GPS PWV increased. The quantitative analysis of the bias and error of the radiosonde PWV data showed that the mean bias decreased in the nighttime except for 2006 winter, and in comparison for summer, RS92-SGP sensor showed the highest quality