3 research outputs found
Immunohistochemical features of progesterone receptors expression of placental barrier in women with multiple pregnancies resulting from assisted reproduction
Hormonal disorders are one of the main known causes of miscarriage and preterm birth in multiple pregnancies resulting from assisted reproductive technology (ART). Progesterone and the number of its receptors play an important role in the preservation and prolongation of pregnancy and it is the pressing issue of our time. The study of placentas, as the main site of synthesis of progesterone, has high informative potential and it is the most important diagnostic object, and information received by its research is essential for the full conclusion on the causes, mechanisms, close and long-term effects of multiple pregnancy pathology.
Aim. The aim of our study was to investigate immunohistochemical features of placentas from women with dichorionic diamniotic twin pregnancies in spontaneous fertilization and after use of assisted reproductive technology (ART).
Methods and results. According to this goal we examined 94 women, 44 of whom had multiple pregnancies due to ART, 42 with separate multiple pregnancy and 38 women with a singleton pregnancy. We carried out clinical and statistical analysis of the course of pregnancy and childbirth in the studied groups.
During the study it was found that multiple pregnancies due to assisted reproduction belong to the high risk of gestation, at which premature births occur much more frequently than in singleton pregnancies. We were the first to carry out the immunohistochemical study of placentas in which the highest expression of progesterone receptors in the nuclei of cells of decidua (45%) related to the parent structure of the placenta from women with multiple pregnancies caused by ART is found. It is also found that with increasing gestational age, there has been a significant decrease in the expression of the activity of progesterone receptors (from 45 to 2.5%), regardless of the method of conception and the number of fetuses.
Conclusions. The results of the study point to the definitive link of structures of placental-endometrial relations as an important component of the appropriateness of hormone therapy
ΠΠΎΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ ΡΠ° ΡΠΌΡΠ½ΠΎΠ³ΡΡΡΠΎΡ ΡΠΌΡΡΠ½Ρ ΠΎΡΠΎΠ±Π»ΠΈΠ²ΠΎΡΡΡ ΠΏΠ»Π°ΡΠ΅Π½ΡΠ°ΡΠ½ΠΎ-Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΠ°Π»ΡΠ½ΠΈΡ ΡΡΡΡΠΊΡΡΡ ΠΏΡΠΈ Π΄ΠΎΠ±ΡΠΎΡΠΊΡΡΠ½ΠΈΡ Ρ Π·Π»ΠΎΡΠΊΡΡΠ½ΠΈΡ ΠΏΡΡ Π»ΠΈΠ½Π°Ρ Ρ ΠΆΡΠ½ΠΎΠΊ
Aim. Researchmaterials were the placenta of women operated on papillary carcinoma (encapsulated), endometrium of women with the same tumor markers detected in the placenta, this endometrium were taken during diagnosing postpartum catamnesis of the state of reproductive health, in comparison to the endometrium of women with benign uterine tumors (leiomyoma).Methods and results. Methods, that were used in the study: histological, immunohistochemical β indirect streptavidin-peroxidase method for detecting the expression of Ki67 Clon MIB-1, p53 Clon: DO-7, REA, Cytoceratina AE1 / AE3; Vimentin Clone: v9, receptors for estrogen (RE) and progesterone (RP). The study evaluated predictors of placental-endometrial disorders in women with cancer pathology: elevation of relative amount of expression of proliferative marker Ki-67 and p53 tumor marker in the placenta and endometrium, leading to violations in the structure of the process of regeneration; dischronosis in levels of expression of estrogen and progesterone receptors in the endometrium; presence of CEA expression in 25% of women of the main group in the endometrium and placenta; atypical glandular hyperplasia, endometrial polyps and micropolyps were found, which are important in the development of oncologic pathology.Π‘ ΡΠ΅Π»ΡΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΎΠ² ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠΎΡΡΠΎΡΠ½ΠΈΠΉ Π² ΠΏΠ»Π°ΡΠ΅Π½ΡΠ΅ ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΆΠ΅Π½ΡΠΈΠ½, ΠΏΡΠΎΠΎΠΏΠ΅ΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΠΎ ΠΏΠΎΠ²ΠΎΠ΄Ρ ΡΠ°ΠΊΠ° ΡΠΈΡΠΎΠ²ΠΈΠ΄Π½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, Π²ΡΠΏΠΎΠ»Π½ΠΈΠ»ΠΈ ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΠ»Π°ΡΠ΅Π½Ρ ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ ΠΆΠ΅Π½ΡΠΈΠ½, ΠΊΠΎΡΠΎΡΡΠΌ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ΅ Π²ΠΌΠ΅ΡΠ°ΡΠ΅Π»ΡΡΡΠ²ΠΎ Π² ΡΠ²ΡΠ·ΠΈ Ρ Π½Π°Π»ΠΈΡΠΈΠ΅ΠΌ ΠΏΠ°ΠΏΠΈΠ»Π»ΡΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ (ΠΈΠ½ΠΊΠ°ΠΏΡΡΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΠΎΠΉ), ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ ΠΎΡ ΠΆΠ΅Π½ΡΠΈΠ½ Ρ Π΄ΠΎΠ±ΡΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠΌΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ ΠΌΠ°ΡΠΊΠΈ (Π»Π΅ΠΉΠΎΠΌΠΈΠΎΠΌΠ°). Π ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½Ρ ΠΎΠ±ΡΠ΅Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Ρ ΠΠΠΠ’ Ki67 Clon MIB-1, Ρ53 Clon DO-7, Π EA, CytoΡeratina AE1/AE3, Vimentin Clone v9, ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΠΊ ΡΡΡΡΠΎΠ³Π΅Π½Π°ΠΌ ΠΈ ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Ρ. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Ρ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΡ ΠΏΠ»Π°ΡΠ΅Π½ΡΠ°ΡΠ½ΠΎ-ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠ°Π»ΡΠ½ΠΈΡ
Π½Π°ΡΡΡΠ΅Π½ΠΈΠΉ ΠΏΡΠΈ ΠΎΠ½ΠΊΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Ρ ΠΆΠ΅Π½ΡΠΈΠ½: ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΠ΅ΠΌΠ° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π² ΠΏΠ»Π°ΡΠ΅Π½ΡΠ΅ ΠΈ ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° ΠΡ-67 ΠΈ ΠΎΠ½ΠΊΠΎΠΌΠ°ΡΠΊΠ΅ΡΠ° Ρ53, ΡΡΠΎ ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΡ ΠΊ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΠΏΡΠΎΡΠ΅ΡΡΠΎΠ² ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΈΠΈ; Π΄ΠΈΡΡ
ΡΠΎΠ½ΠΎΠ· ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠΎΠ² ΡΡΡΡΠΎΠ³Π΅Π½ΠΎΠ² ΠΈ ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Π° Π² ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΈ; Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π ΠΠ Ρ 25% ΠΆΠ΅Π½ΡΠΈΠ½ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ ΠΊΠ°ΠΊ Π² ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΠΈ, ΡΠ°ΠΊ ΠΈ Π² ΠΏΠ»Π°ΡΠ΅Π½ΡΠ΅; Π½Π°Π»ΠΈΡΠΈΠ΅ Π°ΡΠΈΠΏΠΈΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·ΠΈΡΡΠΎΠΉ Π³ΠΈΠΏΠ΅ΡΠΏΠ»Π°Π·ΠΈΠΈ, ΠΏΠΎΠ»ΠΈΠΏΠΎΠ·Π° ΠΈ ΠΌΠΈΠΊΡΠΎΠΏΠΎΠ»ΠΈΠΏΠΎΠ·Π° ΡΠ½Π΄ΠΎΠΌΠ΅ΡΡΠΈΡ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΈΠΌΠ΅ΡΡ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅ Π² ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΠΎΠ½ΠΊΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ.Π ΠΌΠ΅ΡΠΎΡ Π²ΠΈΠ·Π½Π°ΡΠ΅Π½Π½Ρ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΡΠ² ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
ΡΡΠ°Π½ΡΠ² Ρ ΠΏΠ»Π°ΡΠ΅Π½ΡΡ ΡΠ° Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ ΠΆΡΠ½ΠΎΠΊ, ΡΠΊΠΈΡ
ΠΏΡΠΎΠΎΠΏΠ΅ΡΡΠ²Π°Π»ΠΈ Π· ΠΏΡΠΈΠ²ΠΎΠ΄Ρ ΡΠ°ΠΊΡ ΡΠΈΡΠΎΠ²ΠΈΠ΄Π½ΠΎΡ Π·Π°Π»ΠΎΠ·ΠΈ, Π·Π΄ΡΠΉΡΠ½ΠΈΠ»ΠΈ ΠΏΠΎΡΡΠ²Π½ΡΠ»ΡΠ½Π΅ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ ΠΏΠ»Π°ΡΠ΅Π½Ρ ΡΠ° Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ ΠΆΡΠ½ΠΎΠΊ, ΡΠΊΠΈΠΌ Π·Π΄ΡΠΉΡΠ½ΠΈΠ»ΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠ²Π½Π΅ Π²ΡΡΡΡΠ°Π½Π½Ρ Ρ Π·Π²βΡΠ·ΠΊΡ Π· Π½Π°ΡΠ²Π½ΡΡΡΡ ΠΏΠ°ΠΏΡΠ»ΡΡΠ½ΠΎΡ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠΈ (ΡΠ½ΠΊΠ°ΠΏΡΡΠ»ΡΠΎΠ²Π°Π½ΠΎΡ), ΠΉ Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ Π²ΡΠ΄ ΠΆΡΠ½ΠΎΠΊ ΡΠ· Π΄ΠΎΠ±ΡΠΎΡΠΊΡΡΠ½ΠΈΠΌΠΈ ΠΏΡΡ
Π»ΠΈΠ½Π°ΠΌΠΈ ΠΌΠ°ΡΠΊΠΈ (Π»Π΅ΠΉΠΎΠΌΡΠΎΠΌΠ°). ΠΡΠΎΡΡΠ³ΠΎΠΌ Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Π½Ρ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π»ΠΈ Π·Π°Π³Π°Π»ΡΠ½ΠΎΠ³ΡΡΡΠΎΠ»ΠΎΠ³ΡΡΠ½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈ, Π° ΡΠ°ΠΊΠΎΠΆ ΡΠΌΡΠ½ΠΎΠ³ΡΡΡΠΎΡ
ΡΠΌΡΡΠ½ΠΈΠΉ ΠΌΠ΅ΡΠΎΠ΄ Π²ΠΈΡΠ²Π»Π΅Π½Π½Ρ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Π· ΠΠΠΠ’ Ki67 Clon MIB-1, Ρ53 Ρ53 Clon DO-7, Π EA, CytoΡeratina AE1/AE3, Vimentin Clone v9, ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΡΠ² Π΄ΠΎ Π΅ΡΡΡΠΎΠ³Π΅Π½ΡΠ² Ρ ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Ρ. ΠΡΡΠ°Π½ΠΎΠ²ΠΈΠ»ΠΈ ΠΏΡΠ΅Π΄ΠΈΠΊΡΠΎΡΠΈ ΠΏΠ»Π°ΡΠ΅Π½ΡΠ°ΡΠ½ΠΎ-Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΠ°Π»ΡΠ½ΠΈΡ
ΠΏΠΎΡΡΡΠ΅Π½Ρ ΠΏΡΠΈ ΠΎΠ½ΠΊΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡ Ρ ΠΆΡΠ½ΠΎΠΊ: Π·Π±ΡΠ»ΡΡΠ΅Π½Π½Ρ Π²ΡΠ΄Π½ΠΎΡΠ½ΠΎΠ³ΠΎ ΠΎΠ±ΡΡΠ³Ρ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Ρ ΠΏΠ»Π°ΡΠ΅Π½ΡΡ ΡΠ° Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ ΠΏΡΠΎΠ»ΡΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠΊΠ΅ΡΠ° ΠΡ-67 ΡΠ° ΠΎΠ½ΠΊΠΎΠΌΠ°ΡΠΊΠ΅ΡΠ° Ρ53, ΡΠΎ ΠΏΡΠΈΠ·Π²ΠΎΠ΄ΠΈΡΡ Π΄ΠΎ ΠΏΠΎΡΡΡΠ΅Π½Π½Ρ ΠΏΡΠΎΡΠ΅ΡΡΠ² ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΡΡ; Π΄ΠΈΡΡ
ΡΠΎΠ½ΠΎΠ· ΠΏΠΎΠΊΠ°Π·Π½ΠΈΠΊΡΠ² Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΡΠ² Π΄ΠΎ Π΅ΡΡΡΠΎΠ³Π΅Π½ΡΠ² Ρ ΠΏΡΠΎΠ³Π΅ΡΡΠ΅ΡΠΎΠ½Ρ Π² Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ; Π½Π°ΡΠ²Π½ΡΡΡΡ Π΅ΠΊΡΠΏΡΠ΅ΡΡΡ Π ΠΠ Ρ 25% ΠΆΡΠ½ΠΎΠΊ ΠΎΡΠ½ΠΎΠ²Π½ΠΎΡ Π³ΡΡΠΏΠΈ ΡΠΊ Π² Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ, ΡΠ°ΠΊ Ρ Π² ΠΏΠ»Π°ΡΠ΅Π½ΡΡ; Π½Π°ΡΠ²Π½ΡΡΡΡ Π°ΡΠΈΠΏΠΎΠ²ΠΎΡ Π·Π°Π»ΠΎΠ·ΠΈΡΡΠΎΡ Π³ΡΠΏΠ΅ΡΠΏΠ»Π°Π·ΡΡ, ΠΏΠΎΠ»ΡΠΏΠΎΠ·Ρ ΡΠ° ΠΌΡΠΊΡΠΎΠΏΠΎΠ»ΡΠΏΠΎΠ·Ρ Π΅Π½Π΄ΠΎΠΌΠ΅ΡΡΡΡ, ΡΠΊΡ ΠΌΠ°ΡΡΡ Π·Π½Π°ΡΠ΅Π½Π½Ρ Π² ΡΠΎΠ·Π²ΠΈΡΠΊΡ ΠΎΠ½ΠΊΠΎΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΡΡ.
Morphological and immunohistochemical peculiarities of placental-endometrial structures in women with benign and malignant tumors
Aim. Researchmaterials were the placenta of women operated on papillary carcinoma (encapsulated), endometrium of women with the same tumor markers detected in the placenta, this endometrium were taken during diagnosing postpartum catamnesis of the state of reproductive health, in comparison to the endometrium of women with benign uterine tumors (leiomyoma).
Methods and results. Methods, that were used in the study: histological, immunohistochemical β indirect streptavidin-peroxidase method for detecting the expression of Ki67 Clon MIB-1, p53 Clon: DO-7, REA, Cytoceratina AE1 / AE3; Vimentin Clone: v9, receptors for estrogen (RE) and progesterone (RP). The study evaluated predictors of placental-endometrial disorders in women with cancer pathology: elevation of relative amount of expression of proliferative marker Ki-67 and p53 tumor marker in the placenta and endometrium, leading to violations in the structure of the process of regeneration; dischronosis in levels of expression of estrogen and progesterone receptors in the endometrium; presence of CEA expression in 25% of women of the main group in the endometrium and placenta; atypical glandular hyperplasia, endometrial polyps and micropolyps were found, which are important in the development of oncologic pathology