a. <i>Streptococcus salivarius</i> colony forming units obtained on Mitis Salivarius agar over duration of the study from the saliva of subjects that received differing doses of <i>S. salivarius</i> M18 (error bars denote ±SD).

<p>b. <i>Streptococcus salivarius</i> colony forming units obtained on Mitis Salivarius agar containing streptomycin as also used as a selective marker for the probiotic strain (error bars denote ±SD).</p

Total proportion of bacteriocin-like inhibitory substance producing <i>Streptococcus salivarius</i> in saliva samples in all subjects tested prior to probiotic treatment.

<p>Total proportion of bacteriocin-like inhibitory substance producing <i>Streptococcus salivarius</i> in saliva samples in all subjects tested prior to probiotic treatment.</p

The extent of co-aggregation of <i>S. salivarius</i> to periodontal organisms.

<p>“–“ Indicates no co-aggregation, evenly turbid suspension, “+’”weak precipitation with evenly turbid supernatant, “++”moderate precipitation with evenly turbid supernatant and evidence of flocculation “+++”substantial precipitation with clear supernatant and some flocculation, () brackets indicates half-way between number of indicated ‘+’ and the number above it.</p

Each bar represents a single saliva sample and each cluster of bars is a single participant (starting at time 0 and sampled once a week for up to 4 weeks total).

<p>The colored segments represent the relative fraction of each bacterial taxon detected at 1% relative abundance or greater (twenty most predominant OTU shown). Sequences at less than 1% abundance have been included in the ‘‘remainder’’ fraction at the top of the bar (see color legend of bacterial taxa).</p

a. Mean number of <i>S. salivarius</i> M18 detected in saliva samples at different time points receiving different probiotic doses (error bars denote ±SD).

<p>b. Percentage of subjects with <i>S. salivarius</i> M18 detected in saliva samples.</p

The distribution of <i>cspA</i>, <i>cspB</i>, <i>orf</i> 166 and <i>orf</i> 176 in <i>S. salivarius</i> strains having differing adhesion capabilities to HEp-2 cells.

<p>“+” indicates positive for the gene and “−” indicates negative for the gene. Bolded strains are wild type.</p

Relative adhesive abilities of <i>Streptococcus salivarius</i> strains to HEp-2 cells with different megaplasmid combinations.

<p>(Error bars denote ±SD, the method of Turkey was used for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001) and significance shown where the K12 megaplasmid was inserted into non adherent M18 strain.</p

Diversity of the salivary microbiota and if either high or low M18 probiotic persistence had a significant impact. Box plots show weighted UniFrac distances.

<p>Diversity of the salivary microbiota and if either high or low M18 probiotic persistence had a significant impact. Box plots show weighted UniFrac distances.</p

Supplementary materials from Data availability, reusability and analytic reproducibility: evaluating the impact of a mandatory open data policy at the journal Cognition

Additional methodogical information, analyses, and result