High-Throughput Screening of Acyl-CoA Thioesterase I Mutants Using a Fluid Array Platform

Screening target microorganisms from a mutated recombinant library plays a crucial role in advancing synthetic biology and metabolic engineering. However, conventional screening tools have several limitations regarding throughput, cost, and labor. Here, we used the fluid array platform to conduct high-throughput screening (HTS) that identified Escherichia coli ???TesA thioesterase mutants producing elevated yields of free fatty acids (FFAs) from a large (106) mutant library. A growth-based screening method using a TetA-RFP fusion sensing mechanism and a reporter-based screening method using high-level FFA producing mutants were employed to identify these mutants via HTS. The platform was able to cover >95% of the mutation library, and it screened target cells from many arrays of the fluid array platform so that a post-analysis could be conducted by gas chromatography. The ???TesA mutation of each isolated mutant showing improved FFA production in E. coli was characterized, and its enhanced FFA production capability was confirmed

THE DIFFERENCE OF RESIDENT AND NON-RESIDENT CUSTOMER BASED ON SERVICE QUALITY IN THE RESTAURANT BUSINESS USING CART METHODOLOGY

The food industry contributes to a nation’s economic development in many respects. According to the National Restaurant Association in 2009, there are 945,000 restaurants and sales in the restaurant industry are $566 billion a year, which make up about 13million people or 9% of the U.S. workforce food industry. Both the sales and the number of workforce engaged in the food industry have increased steadily since 1970. In other words, as the United States is experiencing remarkable growth in the restaurant business, there is a great deal of competition for surviving in competitive industry

Psychometric properties of a short self-reported measure of medication adherence among patients with hypertension treated in a busy clinical setting in Korea.

BackgroundWe examined the psychometric properties of the Korean version of the 8-item Morisky Medication Adherence Scale (MMAS-8) among adults with hypertension.MethodsA total of 373 adults with hypertension were given face-to-face interviews in 2 cardiology clinics at 2 large teaching hospitals in Seoul, South Korea. Blood pressure was measured twice, and medical records were reviewed. About one-third of the participants (n = 109) were randomly selected for a 2-week test-retest evaluation of reliability via telephone interview.ResultsInternal consistency reliability was moderate (Cronbach α = 0.56), and test-retest reliability was excellent (intraclass correlation = 0.91; P < 0.001), although a ceiling effect was detected. The correlation of MMAS-8 scores with scores for the original 4-item scale indicated that convergent validity was good (r = 0.92; P < 0.01). A low MMAS-8 score was significantly associated with poor blood pressure control (χ(2) = 29.86; P < 0.001; adjusted odds ratio = 5.08; 95% CI, 2.56-10.08). Using a cut-off point of 6, sensitivity and specificity were 64.3% and 72.9%, respectively. Exploratory factor analysis identified 3 dimensions of the scale, with poor fit for the 1-dimensional construct using confirmatory factory analysis.ConclusionsThe MMAS-8 had satisfactory reliability and validity and thus might be suitable for assessment and counseling regarding medication adherence among adults with hypertension in a busy clinical setting in Korea

Analysis of import changes through shift-share, location quotient and BCG techniques: Gwangyang Port in Asia

The main aim of this article is to analyze the import changes of Gwangyang Port using shift-share, location quotient and BCG matrix techniques. We perform the standard shift-share analysis and spatial shift-share analysis for the period 2010–2018 and investigate the import performance of Gwangyang Port for coal, iron ore, natural gas and vegetable matter. The static analysis shows that the regional shift effect, which is the most important component, is negative for coal and iron ore, but positive for natural gas and vegetable matter. The spatial shift-share analysis also indicates that Gwangyang Port experiences not only the gains in regional competitiveness but the industrial advantage for iron ore, natural gas and vegetable matter owing to its higher competitiveness. Incorporating location coefficients into BCG matrix for coal imports, we also show that Gwangyang Port succeeds upgrading its position for natural gas and vegetable matter, but fails escaping from transformation category or upgrading its position for coal and iron ore