Unique features of the transcriptional response to model aneuploidy in human cells

Background: Aneuploidy, a karyotype deviating from multiples of a haploid chromosome set, affects the physiology of eukaryotes. In humans, aneuploidy is linked to pathological defects such as developmental abnormalities, mental retardation or cancer, but the underlying mechanisms remain elusive. There are many different types and origins of aneuploidy, but whether there is a uniform cellular response to aneuploidy in human cells has not been addressed so far. Results: Here we evaluate the transcription profiles of eleven trisomic and tetrasomic cell lines and two cell lines with complex aneuploid karyotypes. We identify a characteristic aneuploidy response pattern defined by upregulation of genes linked to endoplasmic reticulum, Golgi apparatus and lysosomes, and downregulation of DNA replication, transcription as well as ribosomes. Strikingly, complex aneuploidy elicits the same transcriptional changes as trisomy. To uncover the triggers of the response, we compared the profiles with transcription changes in human cells subjected to stress conditions. Interestingly, we found an overlap only with the response to treatment with the autophagy inhibitor bafilomycin A1. Finally, we identified 23 genes whose expression is significantly altered in all aneuploids and which may thus serve as aneuploidy markers. Conclusions: Our analysis shows that despite the variability in chromosome content, aneuploidy triggers uniform transcriptional response in human cells. A common response independent of the type of aneuploidy might be exploited as a novel target for cancer therapy. Moreover, the potential aneuploidy markers identified in our analysis might represent novel biomarkers to assess the malignant potential of a tumor

THE IMPACT OF REHABILITATION ON QUALITY OF LIFE OF PATIENTS WITH ACUTE CORONARY SYNDROME WITH EMERGENCY REVASCULARIZATION BY PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTY AND STENTING THE CORONARY ARTERIES (KRASNOYARSK)

Background. To study the dynamics in the quality of life of patients with acute coronary syndrome (ACS) with emergency revascularization by percutaneous transluminal coronary angioplasty and stenting the coronary arteries (CA). Materials and methods. 150 patients with ACS, percutaneous transluminal coronary angioplasty and stenting the coronary arteries (CA) were included. 65 patients (group 1) performed all procedures of second phase of cardiac rehabilitation program. 85 patients (group 2), who refused of participation in rehabilitation program, were tested according to the test-questionnaire Beck and SF-36. Results. Patients with a full course of rehabilitation program had a statically significant increase in health indicators (physical health component after discharge 42,30 [38,25; 44,35] in 6 months 56,30 [51,85; 5,810], p<0,001; psychological component after discharge 42,80 [39,75; 50,20] in 6 months 56,70 [51,85; 57,20], p<0,001). In group 2 there was no such pattern noticed. Tobacco smoking influenced physical (r = - 0,629, p<0,05) and psychological (r = - 0,621, p<0,05) components of health negatively. Also increased cholesterol influenced physical (r = - 0,424, p<0,05) and psychological (r = - 0,405, p<0,05) components of health. Conclusions. the high importance of rehabilitation program in patients after inpatient ACS treatment, percutaneous transluminal coronary angioplasty and stenting the coronary arteries was revealed. Increased cholesterol and smoking decrease the positive effect of rehabilitation