32 research outputs found
The effect of CD14 and TLR4 gene polimorphisms on asthma phenotypes in adult Turkish asthma patients: a genetic study
The viral aetiology of acute lower respiratory tract infections in hospitalized children younger than 2 years at a university hospital in Istanbul, Turkey
Rutin inceleme ile negatif bulunan beyin omurilik sıvısı örneklerinde Batı Nil Ateşi virusu, Toscana virus ve Lenfositik Koriomenenjit virusunun araştırılması
An early prenatal diagnosis of a 69,XXY case using quantitative fluorescent PCR (QF-PCR) in uncultured amniocytes
Quantitative fluorescent polymerase chain reaction (QF-PCR) has been largely employed for rapid detection of common aneuploidies in prenatal and postnatal diagnosis and consists in DNA amplification by PCR using fluorescent labelled primers and the analysis of chromosome specific small tandem repeats (STR). QF-PCR can rapidly and accurately diagnose sex chromosome status and imbalances, reducing maternal anxiety and aiding in efficient pregnancy management. In this study, an early diagnosis of a 69,XXY case was performed with QF-PCR technique from the uncultured amniocytes. Although, long-term cultures are more common techniques performed in prenatal cytogenetic diagnostic centers, QF-PCR is also an additional molecular reliable technique that enables early prenatal diagnosis. We conclude that, cytogenetic results are important for identifying structural chromosomal abnormalities