Medycyna wspomaganego rozrodu w Polsce – raport za rok 2011 Sekcji Płodności i Niepłodności Polskiego Towarzystwa Ginekologicznego (SPiN PTG)

Aim: The aim of this report is to present data concerning results and complications related to infertility treatment using assisted reproductive technology (ART) and insemination (IUI) in Poland in 2011. Materials and methods: The report was prepared by the Fertility and Sterility Special Interest Group of the Polish Gynaecological Society (SPiN PTG), based on individual data provided by fertility clinics in Poland. Reporting was voluntary and the provided data was not subject to external control. The report presents the availability and the structure of infertility treatment services, the number of procedures performed, their effectiveness and the most common complications. Main results: In 2013, 33 Polish fertility clinics provided information to the SPiN PTG report, presenting data from the year 2011. The total number of reported treatment cycles using ART was 15 340 (incl. 10 011 IVF/ICSI procedures) and 15 627 IUI procedures. The rate of clinical pregnancies in terms of a cycle was 34.2% in case of IVF/ICSI procedures and 13.4% in case of IUI. The prevalence of multiple births was 20.2% and 8.3% respectively, in case of IVF/ICSI and IUI methods. The most frequent complication in the course of treatment using ART was ovarian hyperstimulation syndrome (OHSS). Conclusion: The SPiN PTG report allows to find out the average effectiveness and safety of assisted reproduction technologies and is currently the only proof of responsibility and due diligence of fertility centres in Poland. However, due to the lack of a central register of fertility clinics, facultative participation in the report as well as incomplete information on pregnancy and delivery, the collected data does not reflect the full spectrum of the Polish reproductive medicine.Cel pracy: Celem publikacji jest przedstawienie danych dotyczących wyników i powikłań związanych z leczeniem niepłodności z wykorzystaniem technik wspomaganego rozrodu (ART) i inseminacji (IUI) w Polsce w 2011 roku. Materiał i metody: Raport został opracowany przez Sekcję Płodności i Niepłodności Polskiego Towarzystwa Ginekologicznego (SPiN PTG) w oparciu o dane indywidualne udostępnione przez kliniki leczenia niepłodności zlokalizowane na terenie Polski. Raportowanie miało charakter dobrowolny, a przesłane dane nie podlegały kontroli zewnętrznej. W raporcie przedstawiono dostępność i strukturę usług z zakresu leczenia niepłodności, liczbę przeprowadzonych procedur, ich skuteczność oraz najczęstsze komplikacje. Najważniejsze wyniki: W 2013 roku, przedstawiając dane za 2011 rok, do raportu SPiN PTG przystąpiły 33 polskie kliniki leczenia niepłodności. Odnotowano łącznie 15 340 cykli leczenia z zastosowaniem ART (w tym 10 011 procedur IVF/ICSI) i 15 627 zabiegów IUI. Odsetek ciąż klinicznych w przeliczeniu na cykl wyniósł 34,2% w przypadku procedur IVF/ICSI i 13,4% w przypadku IUI. Wnioski: Raport SPiN PTG pozwala na poznanie średniej skuteczności i bezpieczeństwa technik wspomaganego rozrodu i jest obecnie jedynym dowodem odpowiedzialności i rzetelności medycznej ośrodków zajmujących się leczeniem niepłodności w Polsce. Jednakże ze względu na brak centralnego rejestru klinik leczenia niepłodności, fakultatywny charakter udziału w raporcie oraz braki w monitoringu przebiegu ciąż i porodów zebrane dane nie stanowią pełnego obrazu polskiej medycyny rozrodu

Insights into the Importance of Hydrogen Bonding in the γ-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Serine 236†,‡

The active site of myosin contains a group of highly conserved amino acid residues whose roles in nucleotide hydrolysis and energy transduction might appear to be obvious from the initial structural and kinetic analyses but become less clear on deeper investigation. One such residue is Ser236 (Dictyostelium discoideum myosin II numbering) which was proposed to be involved in a hydrogen transfer network during γ-phosphate hydrolysis of ATP, which would imply a critical function in ATP hydrolysis and motility. The S236A mutant protein shows a comparatively small decrease in hydrolytic activity and motility, and thus this residue does not appear to be essential. To understand better the contribution of Ser236 to the function of myosin, structural and kinetic studies have been performed on the S236A mutant protein. The structures of the D. discoideum motor domain (S1dC) S236A mutant protein in complex with magnesium pyrophosphate, MgAMPPNP, and MgADP·vanadate have been determined. In contrast to the previous structure of wild-type S1dC, the S236A·MgAMPPNP complex crystallized in the closed state. Furthermore, transient-state kinetics showed a 4-fold reduction of the nucleotide release step, suggesting that the mutation stabilizes a closed active site. The structures show that a water molecule approximately adopts the location of the missing hydroxyl of Ser236 in the magnesium pyrophosphate and MgAMPPNP structures. This study suggests that the S236A mutant myosin proceeds via a different structural mechanism than wild-type myosin, where the alternate mechanism is able to maintain near normal transient-state kinetic values

Remarks on the design of trestle bridge galleries in power plants

W artykule przedstawiono problematykę projektowania i realizacji galerii-estakad o przestrzennej konstrukcji prętowej wykorzystywanych w transporcie wewnętrznym między obiektami w elektrowniach.This article describes issues relating to the design and construction of trestle bridge galleries with a spatial rod-based structure used for internal transportation between units at power plants

Damages occurring during use of selected steel constructions and means of their repair

W artykule opisano kilka przykładów uszkodzeń powstałych w trakcie eksploatacji różnego typu konstrukcji stalowych. Uszkodzenia były wynikiem długotrwałej, intensywnej eksploatacji obiektu. Powstały one także z powodu niedoskonałości rozwiązań projektowych i wykonawczych lub wskutek zmian wynikających ze sposobu ich obciążenia. Do każdego z przykładów zamieszczono opis propozycji usunięcia stwierdzonych wad konstrukcji.Several examples of damages occurring during use of different type of steel constructions have been described in this paper. The damage was a result of long-term and intensive use of the structures. Moreover, it occurred due to imperfections in design and its execution or due to change in the load of the object. Each example bas a explanation attached to it describing how to remove specific construction

Cell-penetrating peptides : types and mechanisms of penetration

Before discovery of the Tat peptide over twenty years ago, it was assumed that large peptides and proteins do not penetrate cell membranes. After discovery of Tat, Penetratin [1, 2] and several other peptides of natural origin, able to cross the cell membranes in an energy-independent manner, the structural determinants of their cell-penetrating potency were established, including polycationic character, amphipaticity and presence of proline-rich motifs [3, 4]. Currently known cell-penetrating peptides of natural or synthetic origin are composed of 5 to 40 amino acid residues and belong to one of the three families: oligocationic, amphipatic or proline-rich and penetrate the biological membranes by endocytosis or other ways of direct translocation. Most of these translocation mechanisms are not exclusive and may occur simultaneously, and their contribution may be different for each peptide depending on the conditions (e.g. CPP concentration, pH, etc.). Most CPPs demonstrate little or no mammalian cell toxicity what makes them promising vectors for drug delivery. Such vectors might be useful for efficient internalization of compounds otherways poorly penetrating biological membranes. Novel CPP-effector conjugates may become effective anticancer or antimicrobial agents of a great potential for chemotherapy [3, 5]. In this short review we present a glimpse at the current state of knowledge concerning sub‑families, types and mechanisms of action of most prominent members of CPP family

Assisted reproductive medicine in Poland, 2013–2016: Polish Society of Reproductive Medicine and Embryology (PTMRiE) and Fertility and Sterility Special Interest Group of the Polish Society of Gynaecologists and Obstetricians (SPiN PTGiP) report

Objectives: The purpose of this publication is to present data on the results and complications associated with infertilitytreatment using assisted reproductive technology (ART) and intrauterine insemination (IUI) in Poland between 2013 and 2016.Material and methods: The report was prepared by the Polish Society of Reproductive Medicine and Embryology (PTMRiE)and the Fertility and Sterility Special Interest Group of the Polish Society of Gynaecologists and Obstetrics (SPiN PTGiP) asa part of the European IVF Monitoring program (EIM) for the European Society of Human Reproduction and Embryology(ESHRE). Reporting was voluntary and the data was not subject to external control. The report presents the availability andstructure of infertility treatment services, the number of procedures, their effectiveness and complications.Results: Between 2013 and 2016, a total of 106,718 treatment cycles using ART [64,413 classical in vitro fertilization andin vitro fertilization with intracytoplasmic sperm injection (IVF + ICSI), 36,041 frozen embryo replacements (FER)] and51,405 IUI were recorded. The clinical pregnancy rates per embryo transfer in IVF, ICSI and FER were 38.3%, 38.1% and 32.4%,respectively. The effectiveness of IUI with husband/partner’s semen (IUI-H) was 11.1% and with donor semen (IUI-D) 16.7%.Multiple delivery rates were 11.3% and 6.2% in IVF + ICSI and IUI, respectively. The most common complication was theovarian hyperstimulation syndrome (OHSS) (0.34%).Conclusions: PTMRiE and SPiN PTGiP report is the only national study documenting Polish reproductive medicine. Theresults of infertility treatment effectiveness in Poland are comparable with the European data, complications are less frequentthan in other countries. The low percentage of multiple pregnancies, and so perinatal complications, is especiallyvaluable. However, due to the lack of a central database and register, the possibility of external control and monitoring ofpregnancies and births is limited. Thus, a fully reliable assessment of the treatment quality in our country is not possible