23 research outputs found
HPLC determination of flavonoids in hairy-root cultures of Scutellaria baicalensis Georgi
A simple and sensitive liquid chromatographic method has been
established for determination of the main flavonoid components of
Scutellaria boicolensis Georgi (Lamiaceae) roots and hairy-root
cultures. Wogon, the dried root of the plant, is used in traditional
Chinese medicine for treatment of bronchitis, hepatitis, tumors, and
inflammatory diseases.
Lyophilized hairy roots were extracted with methanol. The crude
extracts were purified by SPE on Supelco LC-8 cartridges. HPLC
separations were performed on a Eurospher 100-C-8 reversed-phase
column. The mobile phase was a gradient prepared from mixtures of
acetonitrile and 0.1% trifluoroacetic acid. Peaks were identified by
addition of standards and/or by diode-array detection. Baicalein
7-O-glucuronide (baicalin), wogonin 7-O-glucuronide (wogonoside),
baicalein, wogonin, and acteoside were determined by the external
standard method at 280 nm. We found that the aglycon (baicalein and
wogonin) content of the transformed roots was consistently higher than
that of the intact root from Siberia
Analysis of polyacetylenes by HPLC in hairy root cultures of Lobelia inflata cultivated in bioreactor
Lobelia inflato L. contains pharmacologically important secondary
metabolites. The main alkaloid is lobeline which has a stimulatory
effect on the respiratory centre. Recently high quantity of
polyacetylenes was determined in the plant and hairy root cultures.
With the aim of increasing of the secondary metabolite production hairy
root cultures were produced (by direct infection of the sterile plants
with Agrobacterium rhizogenes strain R1601). A new HPLC method was
developed for the determination of lobetyolin and lobetyolinin, the
main polyacetylene compounds of L. inflata hairy roots. The analysis
was performed on Hypersil MOS column (250 x 4 mm I.D.). The mobile
phase was 15:85 (v/v) mixture of acetonitrile and water. Peaks were
identified by addition of standards and by DAD. The external
standardized method allowed the determination of lobetyolin and
lobetyolinin with good sensitivity and reliability. This method was
used to measure the polyacetylene contents of hairy roots grew
intensively in bioreactor. High contents of lobetyolin (3.6%) and
lobetyolinin (0.8-1.6%) were detected in the hairy roots because of
their great biosynthetic capacity