Osteoinduction with HA/TCP Ceramics of Different Composition and Porous Structure in Rabbits

AbstractTo determine the effect of material factors on Ca-P biomaterial-induced osteogenesis, six kinds of biphasic calcium phosphate (BCP) ceramics with different HA to TCP ratio (HA/TCP 2-8, 7-3) and different porous structure (micro-, macro- and micro/macro- porous structures) were implanted intramuscularly in rabbits. Different tissue response was detected histologically and microradiographically after the ceramic samples were implanted in the dorsal muscles of rabbits for 3 and 6 months. Obvious bone formation was found in two kinds of ceramics with the same micro/macro- porous structure at both 3 and 6 months. In contrast, no bone formation or host tissue invasion was detected in two other kinds of ceramics with only microporous structure, even after 6 months implantation. Some bone formation was found occasionally in two kinds of ceramics with only macro-porous structure at 6 months. Bone tissue was usually formed in direct contact with the pore surface and was only located in non-dissolved porous regions. Osteocyte lacunae were seen and no pathological calcifications were observed. These results indicate that micro- and macro-porous structure play an important role in the osteoinduction with Ca-P ceramics. Furthermore, the results showed that the osteoinductive capacity of BCP ceramics was influenced by the different dissolution rate through changing HA/TCP ratio

Reliability and validity of ADHD diagnostic criteria in the Assessment System for Individuals with ADHD (ASIA): a Japanese semi-structured diagnostic interview

Background: With reports of a high prevalence of attention-deficit/hyperactivity disorder (ADHD) in adults, publication of ADHD diagnostic criteria in DSM-5, and the urgent need for a relevant diagnostic instrument conforming to DSM-5, we developed the Assessment System for Individuals with ADHD (ASIA), a Japanese semi-structured diagnostic interview. We report here the reliability and validity of ASIA ADHD diagnostic criteria. Methods: ASIA ADHD criterion A corresponds to DSM-5 ADHD criterion A and has 144 original questions assessing nine inattention symptoms and nine hyperactivity-impulsivity symptoms, each having four childhood and four adulthood questions. The 144 questions are evaluated on a 3-point frequency scale. ASIA ADHD criteria B to E correspond to DSM-5 ADHD criteria B to E and are evaluated on a 2-point scale. ASIA was administered to 60 adults (mean age, 29.9 ± 9.0 years; 28 males; 36 ADHD and 24 non-ADHD participants diagnosed by consensus of two experts). Results: For ASIA ADHD criterion A, values of Cronbach's aα for the adulthood and childhood inattention and hyperactivity-impulsivity symptoms ranged from 0.64 to 0.90. Values of κ for two independent raters ranged from 0.98 to 1.00 for the 144 questions and raw agreement rates ranged from 0.97 to 1.00 for criteria B, C, D, and E. The consensus DSM-5 diagnoses endorsed 59 of the 60 ASIA diagnoses (ADHD and non-ADHD). The ADHD group scored significantly higher on 125 of the 144 questions for criterion A than the non-ADHD group. Correlations between ASIA total and subscale scores in adulthood and corresponding scores on the Japanese version of the Conners' Adult ADHD Scales-Self Report were high. Conclusions: ASIA ADHD criteria showed acceptable psychometric properties, although further investigation is necessary. The use of ASIA ADHD criteria could facilitate clinical practice and research into adult ADHD in Japan

A Cross-sectional Multivariate Analysis of the Relationship Between Dental Health and Metabolic Syndrome

Article信州医学雑誌 67(3): 167-181(2019)journal articl

腎盂尿管移行部狭窄症に逆行性エンドピエロトミーを行った小児の1例

右腎盂尿管移行部狭窄症の6歳女児に対し, アキュサイス尿管切開バルーン装置(軟性尿管カテーテル, 7Fr)を用いた逆行性エンドピエトロトミーを行いその実用性を検討した.全身麻酔下でアキュサイスカテーテルを膀胱鏡操作下に腎盂内にまで挿入し, 狭窄部を電気的に切開し更に同部位をバルーンで24Frまで拡張した.切開終了後にエンドピエトロトミー用尿管カテーテル(6/10Fr)を留置し, これを8週間後に抜去した.手術時間は45分間で, 患者は術後3日目に退院した.特に手術に関する合併症はなく, 術後8ヵ月目の経静脈性腎盂撮影と利尿レノグラムによる評価で, 水腎症の程度は改善していたTo determine the feasibility of retrograde endopyelotomy in the management of pediatric ureteropelvic junction (UPJ) obstruction, we treated one girl aged 6 years with the Acucise cutting balloon devise for symptomatic UPJ obstruction. The Acucise catheter (7 Fr, flexible) was placed by a cystoscope over a guide wire with fluoroscopic guidance under general anesthesia. After cutting the stenotic area electronically and dilation until 24 Fr for 10 seconds, a 6/10 Fr endopyelotomy ureteral catheter was left in situ for 8 weeks after the operation. Total operating time was 45 minutes and the child was discharged 3 days after the operation. There were no acute complications and short-term, follow-up 8 months after the operative results were satisfactory as determined by intravenous pyelography and diuretic renogram. UPJ obstruction in children may be treated by retrograde endopyelotomy with the Acucise catheter as well as adults. The principal potential advantage of this procedure is reduced morbidity as compared with antegrade endopyelotomy

Effectiveness of vital staining with iodine solution in reducing local recurrence after resection of dysplastic or malignant oral mucosa

The purpose of this retrospective study was to assess the effect of vital staining with iodine solution in reducing local recurrence after resection of dysplastic or malignant oral mucosa. The historical control group had dysplastic or malignant mucosal lesions resected solely on the evidence of direct inspection and palpation. In the vital staining group tissue was resected only after vital staining with iodine solution. Seven of 25 patients in the conventional group developed recurrent dysplastic or cancerous oral mucosa around the primary site, while no patient among 23 reported recurrence in the vital staining group (p < 0.01). Kaplan Meier assessment showed that the 5-year primary control rate was 100% in the vital staining group and 75% in the conventional group. Although this retrospective study has some limitations, the results suggest that vital staining with iodine may be useful in reducing the incidence of recurrence of dysplastic or cancerous epithelium at a primary site. Further well-controlled study is essential.ArticleBRITISH JOURNAL OF ORAL & MAXILLOFACIAL SURGERY. 50(2):109-112 (2012)journal articl

Synthesis of Boc-(2S, 3R)-3-Amino-2-Hydroxy-4-Phenylbutanoic Acid, a Moiety of Bestatin

Article信州大学農学部紀要 18(1): 103-108(1981)departmental bulletin pape

Effect of single-dose extended-release oral azithromycin on anticoagulation status in warfarinized patients

Objective. The aim of this study was to investigate the possible influence of single-dose 2.0-g azithromycin (AZ-ER) on anticoagulation in patients taking warfarin. Study Design. Eighteen consecutive patients receiving long-term stable warfarin therapy were enrolled in this study. AZ-ER was administered 1 hour prior to tooth extraction. The international normalized ratio (INR) value was measured prior to AZ-ER administration as well as during, 1 day after, and 7 days after the tooth extraction. Additionally, the azithromycin concentration in the extraction wound as well as in the peripheral venous blood was assessed. Results. The changes in INR throughout the study period were not statistically significant (2-factor analysis of variance, NS). The azithromycin concentration in extraction wounds was higher than that in peripheral veins. Conclusions. The results of this study suggest that prophylactic administration of AZ-ER to patients receiving daily warfarin therapy with a stable coagulation status has no relevant effect on the anticoagulant effect of warfarin. (Oral Surg Oral Med Oral Pathol Oral Radiol 2013;115:148-151)ArticleORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY. 115(2):148-151 (2013)journal articl

Intrathecal Administration of the α1 Adrenergic Antagonist Phentolamine Upregulates Spinal GLT-1 and Improves Mirror Image Pain in SNI Model Rats

Mirror image pain (MIP) is a type of extraterritorial pain that results in contralateral pain or allodynia. Glutamate transporter-1 (GLT-1) is expressed in astrocytes and plays a role in maintaining low glutamate levels in the synaptic cleft. Previous studies have shown that GLT-1 dysfunction induces neuropathic pain. Our previous study revealed bilateral GLT-1 downregulation in the spinal cord of a spared nerve injury (SNI) rat. We hypothesized that spinal GLT-1 is involved in the mechanism of MIP. We also previously demonstrated noradrenergic GLT-1 regulation. Therefore, this study aimed to investigate the effect of an α1 adrenergic antagonist on the development of MIP. Rats were subjected to SNI. Changes in pain behavior and GLT-1 protein levels in the SNI rat spinal cords were then examined by intrathecal administration of the α1 adrenergic antagonist phentolamine, followed by von Frey test and western blotting. SNI resulted in the development of MIP and bilateral downregulation of GLT-1 protein in the rat spinal cord. Intrathecal phentolamine increased contralateral GLT-1 protein levels and partially ameliorated the 50% paw withdrawal threshold in the contralateral hind paw. Spinal GLT-1 upregulation by intrathecal phentolamine ameliorates MIP. GLT-1 plays a role in the development of MIPs