Fotografia para extensão e divulgação científica

Laboratório de pesquisa microbiológica da Universidade Federal de São Paulo (Unifesp). Janeiro de 2023. Pesquisador aplicando meio de cultura em placa poliestireno de 96 poços com pipeta multicanal. Documento iconográfico cedido pela pesquisadora Mariana Neri Lucas Kurihara ao curso de extensão 23357 Fotografia para extensão e divulgação científica: captura, descrição e engajamento.Não recebi financiament

Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome

Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs