Effect of temperature, multispecies probiotics mixture (<i>B. subtilis, L. lactis</i> and <i>S. cerevisiae</i>) and their interaction on the blood parameters of <i>L. rohita</i> fingerlings.

<p>Data expressed as Mean ± SE (n = 3). Different superscripts in each column under each sub headings vary significantly (P<0.01).</p><p>T1 (28°C+BF (Basal feed) +PM (Probiotic mixture)), T2 (31°C+BF+PM), T3 (34°C+BF+PM), T4 (37°C+BF+PM), T5 (28°C+BF), T6 (31°C+BF), T7 (34°C+BF) and T8 (37°C+BF).</p

Analysis of the effects of temperature and probiotic supplementation on apoptosis in Kidney (A–C), Gill (D–F) and Liver (G–I).

<p>The apoptotic cells are represented by red color while blue color represents the juxtaposed cell nucleus. Note: Different cell types i.e. nephrons, interstitial cells of kidney, primary gill lamellae, secondary gill lamellae and apoptotic hepatic cells are respectively marked with ▴, $, #, * and ∧. Bar length- 50 micron. J–L shows the relative apoptotic cell density in the kidney, gill and liver at different temperature. Each value represents Mean ±SE of three biological replicates (n = 3), each replicate prepared from four fish. Briefly, the mean data from 4 fish (from same treatment replicate) were used for calculating the biological average apoptotic cell count. The average data generated from three such biological averages (n = 3) were used for statistical analysis. Significance between the treatments was determined by Duncan's Multiple Range Test (DMRT) using SPSS (Version 16.0).</p

Effect of temperature and probiotic supplementation on percentage body weight gain of <i>Labeo rohita</i> fingerlings at the completion of the experiment.

<p>Significance between the treatments was determined by Duncan's Multiple Range Test (DMRT) using SPSS (Version 16.0). Similar alphabets (a,b,c,d) denotes no significance at P<0.01. Each value represents Mean ±SE of three biological replicates (n = 3), each replicate prepared from four fish. Briefly, data from four fish of one treatment replicate were averaged and used as one biological replicate. Similarly, three biological replicates (n = 3) were prepared, each from one treatment replicate.</p