THE EFFECT OF PREIMPLANTATION EMBRYO DEVELOPMENT ARREST ON ASSISTED REPRODUCTIVE TREATMENT RESULTS

Objective: During assisted reproductive treatments, human preimplantation embryo's development can sometimes may stop cleavage at various stages due to in vitro culture media, patient age, ovarian stimulation protocols, and gametes. Implantation failure and abortion can be observed after the transfer of other embryos, which have normal development, in couples with developmental arrest. On the other hand, the underlying causes of embryonic arrest cannot be explained clearly. The aim of this study was to investigate the effect of early embryonic arrest on the success of assisted reproductive techniques

DNA repair gene variants in endometrial carcinoma

Several polymorphisms in the DNA repair gene are thought to have significant effects on cancer risk. In this study, we investigated the association of the polymorphisms in the DNA repair genes, XRCC1 Arg399Gln, XRCC3 Thr241Met, XPD Lys751Gln, XPG Asp1104His, APE1 Asp148Glu, and HOGG1 Ser326Cys, with endometrium cancer risk. Two hundred and sixty-two women were included in the study. Endometrial biopsy was performed, and on the basis of diagnosis and histological examination, women were divided into two groups: a control group (n = 158) and an endometrial cancer group (n = 104). Genotypes were determined by PCR-RFLP assays in endometrial carcinoma patients and age-matched controls. In this study, we found that the frequencies of Glu+ and Asp/Glu genotypes in APE, Gln/Gln genotype of XRCC1, Met/Met genotype of XRCC3, Cys+ and Ser/Cys genotypes of HOGG1, His+ and Asp/His genotypes of XPG, and Gln+ and Gln/Gln genotypes of XPD are more prevalent in patients than controls. Frequencies of Thr/Thr genotype in XRCC3 were increased in controls compared with patients and seem to be protected from endometrial cancer. Our findings suggest that XRCC1, XRCC3, XPD, XPG, APE1, and HOGG1 genetic variants may be associated with endometrial cancer in Turkish women

Genetic variants of SDF-1 and CXCR4 genes in endometrial carcinoma

In this study, we aimed to investigate a possible association between the Stromal cell-derived factor-1 (SDF-1) and CXCR4 polymorphisms and the risk of developing endometrial carcinoma. SDF-1 3'A and CXCR4 gene polymorphisms was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism in 139 healthy individuals and 113 patients with endometrial carcinoma. In our study groups SDF-1 3'A AA genotype frequency was higher in patients that of controls and individuals who had AA genotype showed a 2.6-fold increased risk for endometrial cancer. The carriers of CXCR4 T allele were higher in patients compared with controls and individuals who had TT genotype had a 2.5-fold high risk for endometrial carcinoma. Our finding suggest that there was no significant association between the (SDF-1) and CXCR4 polymorphisms and endometrium cancer risk. Further studies in a larger population are needed to better elucidate the role of (SDF-1) and CXCR4 gene polymorphisms in the risk of endometrial carcinogenesis. To the best of our knowledge, this is the first study to show such an association

Expression of pro-apoptotic and anti-apoptotic proteins in granulosa cells of women with diminished ovarian reserve

Purpose To evaluate the expressions of caspase-3 and cytochrome c and heat shock protein 70 (Hsp70) in granulosa cells (GCs) from women with normal ovarian reserve (NOR) and diminished ovarian reserve (DOR) undergoing intracytoplasmic sperm injection (ICSI). Methods GCs were collected from 117 infertile women during oocyte retrieval. Patients were classified into four groups as follows: DOR-COC score of 0, DOR-COC score of I, NOR-COC score of 0, and NOR-COC score of I. The caspase-3, cytochrome c, and Hsp70 analyses were performed immunohistochemically in GCs. The ICSI outcomes were evaluated prospectively. Results The clinical pregnancy and live birth rates were higher in DOR-COC score of I (15, 30.6%; 14, 38.9%) and NOR-COC score of I (19, 38.77%; 19, 52.7%) groups, compared with DOR-COC score of 0 (12, 24.4%; 3, 6.1%) and NOR-COC score of 0 (3, 6.1%; 0%) groups (p = 0.0001; 0.00002), respectively. Caspase-3 and cytochrome c expression levels were higher in DOR-COC score of 0 (23, 65.7%; 25, 71.4%) and NOR-COC score of 0 groups (19, 61.3%; 20, 64.5%), compared with DOR-COC score of I (8, 32%; 9, 36%) and NOR-COC score of I groups (7, 26.9%; 8, 30.8%) (p = 0.00297; p = 0.002), respectively. Lower expression levels of Hsp70 were found in DOR-COC score of 0 (11, 31.4%) and NOR-COC score of 0 groups (10, 32.3%), compared with DOR-COC score of I (16, 64%) and NOR-COC score of I groups (20, 76.9%) (p = 0.001), respectively. Hsp70 expression levels were positively correlated with the number of day 3 good-quality embryo and negatively correlated with estradiol levels in the DOR group. Conclusion Our data suggest that COC score of 0 is associated with increased expression levels of apoptotic proteins, decreased expression levels of anti-apoptotic protein, and poor ICSI clinical outcomes in women with and without DOR

SERUM MYELOPEROXIDASE LEVEL AND GENE POLYMORPHISM IN ENDOMETRIUM CANCER

WOS: 000260555300237

Prevalence of MMP-3 E45K polymorphism in Turkish patients with endometrial carcinoma

Endometrial carcinoma is a metastatic and recurrent disease has a worse prognosis. We aim to investigate the MMP-3 gene polymorphism in Turkish patients with endometrial carcinoma. In recent years, number of genetic studies have been increased to find new prognostic and theraupeutic markers. Matrix metalloproteinases [MMPs] are proteolytic enzymes that degrade all components of the extracellular matrix which are play an important role of cancer metastasis and invasion. MMP-3, also known as stromelysin-1, to lyse basal membrane collagen and induce the synthesis of some other MMPs. 97 patients with endometrial carcinoma and 100 healthy controls were included in this study. MMP-3 E45K polymorphism was determined using polymerase chain reaction/ restriction fragment length polymorphism in study groups.There were no significant differences between any genotypes or allele in control and patient groups for MMP-3 E45K polymorphism. Besides no significant correlation was found between MMP-3 E45K polymorphisms and clinical characteristics, such as histology, grade, metastasis and family history. ur study suggests that the MMP-3 E45K polymorphism is not associated with endometrial carcinoma process in Turkish patients. [Med-Science 2018; 7(4.000): 762-5

Genetic Variants of Vascular Endothelial Growth Factor and Risk for the Development of Endometriosis

Backround/Aims: Endometriosis is regarded as a complex disese, in which genetic and environmental factors contribute to the disease phenotype. Whether vascular endothelial growth factor (VEGF) -460 C/T and +405 G/C polymorphisms are associated with susceptibility to endometriosis was investigated. Patients and Methods: Diagnosis of endometriosis was made on the basis of laparoscopic findings. Stage of endometriosis was determined according to the Revised American Fertility Society classification. Sixty out of the 112 women enrolled had no endometriosis, 11 had mild or early-stage endometriosis and 41 had severe endometriosis. Polymerase chain reaction (PCR), restriction fragment length polymorphism and agarose gel electrophoresis techniques were used to determine the -460 C/T and +405 GIG genotypes. Results: The VEGF +405 GIG genotype frequencies among the cases and controls were CC 55.8% and 35%; GC 30.8% and 50.0%; GG 13.5% and 15.0%, respectively. The allelic frequencies were C 71.15% (cases) and 60.0% (controls) and G 28.8% (cases) and 40% (controls). Patients with endometriosis had a higher incidence of the VEGF +405 CC genotype compared with the controls (p=0.027). Women with VEGF +405 CC genotype had 2.3-fold higher risk for endometriosis. VEGF +405 GC genotype and G allele in the control group was higher than the endometriosis group (p=0.039, p=0.027 respectively). The VEGF 460 C/T genotype frequencies among the cases were CC 21.2%, CT 26.9% and IT 51.9%; the C and T allelic frequencies were 34.6% and 65.3%, respectively. The VEGF -460 genotype frequencies among the controls were CC 31.70%, CT 18.3% and TT 50.0%; the C and T allelic frequencies were 40.8% and 59.1%, respectively (p>0.05). There was linkage disequilibrium between VEGF -460 C/T and +405 GIG polymorphisms (D': 0.197, r(2)=0.013). We observed that the VEGF 460T/405C haplotype frequency was significantly higher in patients compared to controls (p=0.011). Conclusion: Our data suggest that the CC genotype of VEGF +405 and 460T/405C haplotypes of VEGF may be associated with the risk of endometriosis, but the G allele of VEGF +405 appears to be protective against endometriosis

RNA ISOLATION AND DETECTION OF CELLULAR RNA QUANTITY OF SPERMATOZOA AND EMBRYOS PRIOR TO GENE EXPRESSION ANALYSES

Objective: Biological samples that are analyzed in reproductive biology are generally rare, difficult to obtain, and a nonreplicable group of cells. Furthermore, investigating low numbers of cells requires modifications to the routine methods used in genetic analyses. The aim of our study was to improve RNA isolation methods for obtaining a sufficient amount of total RNA from spermatozoa and embryo samples for downstream gene expression analyses