CadC-mediated activation of the cadBA promoter in Escherichia coli

The transcriptional activator CadC in Escherichia coli, a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB. cadBA is induced under conditions of acidic external pH and exogenous lysine; anoxic conditions raise the expression level up to 10 times. To characterize the binding mechanism of CadC, procedures for the purification of this membrane-integrated protein and its reconstitution into proteoliposomes were established. The binding sites of CadC upstream of the cadBA promoter region were determined by in vitro DNaseI protection analysis. Two regions were protected during DNaseI digestion, one from - 144 to - 112 bp, designated Cad1, and another one from - 89 to - 59 bp, designated Cad2. Binding of purified CadC to Cad1 and Cad2 was further characterized by DNA-binding assays, indicating that CadC was able to bind to both DNA fragments. Genetic analysis with promoter-lacZ fusions confirmed that both sites, Cad1 and Cad2, are essential for activation of cadBA transcription. Moreover, these experiments revealed that binding of H-NS upstream of the CadC-binding sites is necessary for repression of cadBA expression at neutral pH and under aerobic conditions. Based on these results, a model for transcriptional regulation of the cadBA operon is proposed, according to which H-NS is involved in the formation of a repression complex under non-inducing conditions. This complex is dissolved by binding of CadC to Cad1 under inducing conditions. Upon binding of CadC to Cad2 cadBA expression is activated. Copyright (C) 2005 S. Karger AG, Basel

Positive and Negative Ion Motion in Thermal Oxide on Silicon by Radiochemical and MOS ANALYSIS

Metal-oxide-silicon capacitor and radiochemical analyses of anion in sodium contamination failure mod

Validating foundry technologies for extended mission profiles

This paper presents a process qualification and characterization strategy that can extend the foundry process reliability potential to meet specific automotive mission profile requirements. In this case study, data and analyses are provided that lead to sufficient confidence for pushing the allowed mission profile envelope of a process towards more aggressive (automotive) applications.\ud \u

Effect of Thermal Gradients on the Electromigration Lifetime in Power Electronics

The combined effects of electromigration and thermomigration are studied. Significantly shorter electromigration lifetimes are observed in the presence of a temperature gradient. This cannot be explained by thermomigration only, but is attributed to the effect of temperature gradient on electromigration-induced failures

Instability due to long range Coulomb interaction in a liquid of polarizable particles (polarons, etc.)

The interaction Hamiltonian for a system of polarons a la Feynman in the presence of long range Coulomb interaction is derived and the dielectric function is computed in mean field. For large enough concentration a liquid of such particles becomes unstable. The onset of the instability is signaled by the softening of a collective optical mode in which all electrons oscillate in phase in their respective self-trapping potential. We associate the instability with a metallization of the system. Optical experiments in slightly doped cuprates and doped nickelates are analyzed within this theory. We discuss why doped cuprates matallize whereas nickelates do not.Comment: 5 pages,1 figur

Semantic programming model-based design

For a generic flexible efficient array antenna receiver platform a hierarchical tiled architecture has been proposed, giving a heterogeneous multi-processor system-on-chip (MPSoC), multiple chips on a board (MCoB) and multiple boards in a system (MBiS). A wide range of MPSoCs are predicted to be used in the near future but how to efficiently apply these designs remains an issue. We will advocate a model-based design approach and propose a single semantic (programming) model for representing the specification, design and implementation and allowing for verification, simulation, architecture definition and design space exploration.\ud \ud A single model for specification, (formal or functional) verification, simulation and programming an MPSoC has obvious as well as some less obvious advantages. It allows for model-based design down to the implementation, especially for hierarchical MPSoC architectures. Partitioning and mapping of the functionality to an architecture is commonly done manually. Using the proposed approach the feasibility of (partly) automated design space exploration is discussed for determining either a partitioning and mapping for a given architecture or an optimal architecture based on set constraints.\ud \ud The proposed hierarchical tiled architecture provides a flexible reconfigurable solution, however partitioning, mapping, modeling and programming such systems remains an issue. The proposed approach tackles these problems at a higher conceptual level, thereby exploiting the inherent composability and parallelism available in the formalism. Design space explorations is facilitated by allowing transformations between different partitionings and mappings. However, the generic applicability and limitations of this approach will need to be researched further.\ud \u

Model ab initio study of charge carrier solvation and large polaron formation on conjugated carbon chains

Using long C_{N}H_{2} conjugated carbon chains with the polyynic structure as prototypical examples of one-dimensional (1D) semiconductors, we discuss self-localization of excess charge carriers into 1D large polarons in the presence of the interaction with a surrounding polar solvent. The solvation mechanism of self-trapping is different from the polaron formation due to coupling with bond-length modulations of the underlying atomic lattice well-known in conjugated polymers. Model ab initio computations employing the hybrid B3LYP density functional in conjunction with the polarizable continuum model are carried out demonstrating the formation of both electron- and hole-polarons. Polarons can emerge entirely due to solvation but even larger degrees of charge localization occur when accompanied by atomic displacements

[Accepted Manuscript] Field testing a draft version of the UNICEF/Washington Group Module on child functioning and disability. Background, methodology and preliminary findings from Cameroon and India

Background Global child disability data are generally non-comparable, comprising different tools, methodologies and disability definitions. UNICEF and The Washington Group on Disability Statistics (WG) have developed a new tool on child functioning and disability to address this need. Aims The aim of this paper is to describe the development of the new module, and to present an independent field test of the draft module in two contrasting settings. Methods UNICEF and the WG developed a parent-reported survey module to identify children aged 2�17 years with functional difficulties in population-based surveys through: review of existing documentation, consultation with experts and cognitive testing. A field test of the draft module was undertaken in Cameroon and India within a population-based survey. Functional limitation in each of 14 domains was scored on a scale comprising �no difficulty�, �some difficulty�, �a lot of difficulty� and �cannot do�. Results In all, 1713 children in Cameroon and 1101 children in India were assessed. Sixty-four percent of children in Cameroon and 35% of children in India were reported to have at least some difficulty in one or more domain. The proportion reported to have either �a lot of difficulty� or �cannot do� was 9% in Cameroon and 4% in India. There were no significant differences in reported functional difficulties by sex but children aged 2�4 were reported to have fewer functional difficulties of any kind compared with older children in both countries. Conclusion Comparable estimates were generated between the two countries, providing an initial overview of the tool's outputs. The continued development of this standardised questionnaire for the collection of robust and reliable data on child disability is essential