Oestrous cyclicity disruption in Wistar rats subjected to partial sleep deprivation

Background: Chronic partial sleep deprivation continues to be a global problem. Studies have shown that oestrous cyclicity is disrupted by total sleep deprivation. The relationship between partial sleep deprivation – the predominant form of sleep deprivation in the current global society – and the oestrous pattern remains elusive. This study examined oestrous cycle patterns of rats subjected to partial sleep deprivation.Methods: Thirty female Wistar rats were divided into control and Sleep-Deprived (SD) groups of fifteen rats each. Oestrous patterns were monitored for two weeks before sleep deprivation and throughout the study. Sleep deprivation was induced using the modified multiple platform method. Five animals were sacrificed under thiopental anaesthesia during proestrus on days 7, 14 and 21. The ovaries, uteri and adrenal glands were examined. Data were compared using t-test at p<0.05.Results: Proestrus and oestrus frequencies reduced in the SD groups; metestrus frequency increased in the 7 days and 14 days SD and diestrus frequency increased in 21 days SD group. Cycle lengths increased in the 14 days and 21days SD groups. The SD groups had inflammation of ovary and uterus, as well as adrenal medulla hyperplasia.Conclusion: Partial sleep deprivation reduces frequencies of phases characterized by sexual activity and ovulation while increasing cycle lengths in Wistar rats. Thus, adequate sleep is important for women who are trying to get pregnant.Keywords: Sleep deprivation, Oestrous cycle, Adrenal medulla, rat

Coconut water prevents renal and hepatic changes in offspring of monosodium glutamate-treated Wistar rat dams

Summary: Monosodium glutamate (MSG) is a widely-consumed taste enhancer which has been implicated in the aetiology of renal and hepatic dysfunction in adults and their offspring. There is increasing evidence on the therapeutic properties of Coconut Water (CW) in kidney and liver disorders. This study investigated the effects of CW on renal and hepatic functions in offspring of MSG-fed dams. Twelve female Wistar rats (120 – 140 g) were grouped into four as follows; Control (10 ml/Kg distilled water), MSG (0.08 mg/Kg), CW (10 ml/Kg) and MSG+CW. Treatments were given orally daily commencing two weeks prior to mating, throughout mating and gestation until parturition. All dams received standard rodent diet and drinking water ad libitum throughout the study. After weaning on Post-Natal Day (PND) 28, serum was obtained from offspring for assay of liver and renal function. Histological analysis of the livers and kidneys were performed on both dams and offspring. There was no significant difference in liver enzymes, urea, creatinine and albumin levels amongst the offspring on PND 28. However, liver and kidney sections from MSG dams and their offspring showed early degenerative changes which were not evident in renal and hepatic tissues from CW and MSG+CW dams and offspring. These observations suggest that coconut water protects against monosodium glutamate-induced renal and hepatic dysfunction in dams and offspring.Keywords: Monosodium glutamate, Cocos nucifera water, Foetal programming, Kidney, Live

Formulation of tablets of Ficus capensis leaves - a potential anti-ulcer drug

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Comparative study of some mechanical and release properties of paracetamol tablets formulated with cashew tree gum, povidone and gelatin as binders

The mechanical and release properties of paracetamol tablets formulated with cashew gum (CAG), povidone (PVP) and gelatin (GEL) as binders were studied and compared. The parameters studied were tensile strength (TS), brittle fracture index (BFI), friability (F), disintegration time (DT) and percentage drug released (PDR). Results showed that the TS and BFI values of tablets formulated with CAG were the lowest at all binder concentrations. The friabilities of all formulations were within accepted limits