Molecular characterization of microbial communities in fault-bordered aquifers in the Miocene formation of northernmost Japan

We investigated the diversity and distribution of archaeal and bacterial 16S rRNA gene sequences in deep aquifers of mid- to late Miocene hard shale located in the northernmost region of the Japanese archipelago. A major fault in the north-west-south-east (NW-SE) direction runs across the studied area. We collected three groundwater samples from boreholes on the south-west (SW) side of the fault at depths of 296, 374 and 625 m below ground level (m.b.g.l.) and one sample from the north-east (NE) side of the fault at a depth of 458 m.b.g.l. The groundwater samples were observed to be neutral and weakly saline. The total microbial counts after staining with acridine orange were in the order 105-106 cells mL-1 and 103 cells mL-1 in the aquifers to the SW and to the NE of the fault, respectively. A total of 407 archaeal and bacterial 16S rRNA gene sequences (204 and 203 sequences, respectively) were determined for clone libraries constructed from all groundwater samples. Phylogenetic analyses showed that the libraries constructed from the SW aquifers were generally coherent but considerably different from those constructed from the NE aquifer. All of the archaeal clone libraries from the SW aquifers were predominated by a single sequence closely related to the archaeon Methanoculleus chikugoensis, and the corresponding bacterial libraries were mostly predominated by the sequences related to Bacteroidetes, Firmicutes and δ-Proteobacteria. In contrast, the libraries from the NE aquifer were dominated by uncultured environmental archaeal clones with no methanogen sequences and by β-proteobacterial clones with no sequences related to Bacteroidetes and δ-Proteobacteria. Hence, the possible coexistence of methanogens and sulphate reducers in Horonobe deep borehole (HDB) on the SW side is suggested, particularly in HDB-6 (374 m.b.g.l.). Moreover, these organisms might play an important geochemical role in the groundwater obtained from the aquifers

Evaluation of faults stability due to passing seismic waves: Study case of groundwater level changes induced by the 2011 Tohoku earthquake in Central Japan

In the study, we analyze changes in groundwater pressure observed in several boreholes drilled in and around the Mizunami Underground Research Laboratory (MIU) induced by the 2011 off the Pacific coast of Tohoku Earthquake (Mw 9.0). The aim of this project is a development of methodology to evaluate systematic fault activity by numerical analysis. To reach this goal we investigate the behavior of the fault zones present in the area during the passing of seismic waves. We built a simplified hydrogeological model of the MIU site and performed a series of fluid flow simulations with TOUGH2 flow numerical code. We investigate how changes in permeability along three faults present in the study area: the Tsukiyoshi Fault, the Hiyoshi Fault and the Main-Shaft Fault may have influence the groundwater level monitored in boreholes intervals. We also test the influence of the cone of depression at the MIU site and the hydraulic connectivity between the sedimentary cover and the granite aquifers. Our results suggest that two main mechanisms are responsible for the observed changes in groundwater pressure: (1) crustal dilation induced by the Tohoku earthquake causing a groundwater recharge from the sedimentary aquifers to the Toki granite aquifer where the sedimentary cover is thick; and (2) permeability increase along faults critically oriented for shear reactivation and oriented in the direction of the passing seismic wave. In this case, the seismic wave increases the shear stress acting on the fault promoting slip and a change in permeability through a mechanism of slip-induced dilation. Faults not critically stressed and faults critically oriented for shear reactivation but oriented perpendicular to the passing seismic wave are not reactivated