The Effect of Insulin Administration and Antibacterial Irrigation with Chlorhexidine Gluconate on the Disturbance of Periodontal Tissue Caused by Food Impaction in Streptozotocin-Induced Diabetic Rats

福岡歯科大学2015年

Disturbance of Periodontal Tissue Caused by Mechanical Compression into the Gingival Col of Streptozotocin-induced Diabetic Rats

福岡歯科大学2013年

Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.

福岡歯科大学2013年

Tumor-induced lymphangiogenesis in cervical lymph nodes in oral melanoma-bearing mice.

福岡歯科大学2013年

Lupus-like oral mucosal lesions in mercury-induced autoimmune response in Brown Norway rats.

BACKGROUND:Administration of mercury at nontoxic doses induces systemic autoimmune disease in Brown Norway (BN) rats. The pathogenesis of lupus-like oral mucosal lesion by mercury-induced autoimmunity is still unclear, even though the oral mucosa is observed to be commonly affected in mercury-treated BN rats. In this study, we investigated the immunopathology of lupus-like oral mucosal lesions in a model of mercury-induced systemic autoimmunity.METHODS:Brown Norway male rats were injected subcutaneously with either phosphate-buffered saline (control) or mercury at a dose of 1.0 mg per kilogram of body weight on days 0, 3, 5, and 7. Blood, kidney, and tongue samples were taken at various timepoints for evaluation by immunohistochemistry, RT-PCR, and lupus band test (LBT).RESULTS:Oral mucosal lesions were classified according to three consecutive temporal phases on the basis of infiltration of immunocompetent cells as follows: (phase I) infiltration of MHC class II+ dendritic cells (DC) and macrophages; (phase II) addition of ED1+ macrophage infiltrates; and (phase III) focal infiltration of pan T cells following increased infiltration of DC and macrophages. Dense infiltration of DC and macrophages was observed in the basement membrane (BM) zone of the oral epithelium. Tissue expression of IL-4 mRNA was detected in early lesions (phase I), suggesting that locally produced IL-4 may be responsible for Th2-mediated immune response. A linear and continuous smooth pattern of fluorescence was observed in the oral epithelial BM in addition to renal glomeruli, indicating immune complex deposits.CONCLUSIONS:Local autoimmune responses are involved in the pathogenesis of mercury-induced lupus-like lesions of the oral mucosa.福岡歯科大学2015年

Solitary neurofibroma of the gingiva with prominent differentiation of Meissner bodies : a case report

<p>Abstract</p> <p>Background</p> <p>Oral neurofibromas are peripheral nerve sheath tumors, similar to schwannomas. Histological variations in oral neurofibromas are relatively uncommon.</p> <p>Case presentation</p> <p>Here, we present a case of unique variation in the observed characteristics of a neurofibroma, with no relation to neurofibromatosis type-1 or von Recklinghausen disease of the skin. The neurofibroma was observed in the right mandibular gingiva of a 32-year-old Japanese woman. Histologically, it differed from conventional neurofibromas in that the tumor was composed of a mixture of fine fibrillary collagen in sheets and/or cords of neoplastic Schwann cells containing numerous clusters of Meissner bodies. Histologically, these bodies were in contact with neoplastic Schwann cells. The Meissner bodies were immunopositive for S-100 protein, neuron-specific enolase, and vimentin, but were negative for calretinin. CD34-positive spindle cells were observed around the Meissner bodies. No recurrence or signs of other tumors have been observed in the patient for 5 years after tumor resection.</p> <p>Conclusion</p> <p>To the best of our knowledge, no formal descriptions of sporadic, solitary neurofibromas containing numerous Meissner bodies occurring in the oral cavity are available in literature. We believe that an uncommon proliferation of Meissner bodies, as seen in the present case, may result from aberrant differentiation of neoplastic Schwann cells.</p

IMMUNOCYTOCHEMICAL LOCAHZATION OF PAROTIN SUBUNIT IN SUBMANDIBULAR GLANDS OF THE JAPANESE MONKEY, MACACA FUSCATA

To determine the intracellular localization of parotin subunit in monkey submandibular glands, parotin subunit antiserum labeling and immunogold labeling were performed at both light and electron microscopic levels. Parotin subunit was found to be distributed along tonofibrils of striated duct cells, and to be localized intensely in middle electron-dense areas and less intensely in the dense core of secretory granules of serous acinar cells. Therefore, parotin subunit is secreted by serous cells and speculated to play a role in the formation of tonofibrils in duct apical region