Supplementary Material for: Exacerbating Factors Induce Different Gene Expression Profiles in Peripheral Blood Mononuclear Cells from Asthmatics, Patients with Chronic Obstructive Pulmonary Disease and Healthy Subjects

<p><b><i>Background:</i></b> Despite several common phenotypic features, chronic obstructive pulmonary disease (COPD) and severe asthma differ with regard to their causative factors and pathophysiology. Both diseases may be exacerbated by environmental factors, however, the molecular profiles of disease episodes have not been comprehensively studied. We identified differences in gene and protein expression profiles expressed by peripheral blood mononuclear cells (PBMC) of COPD patients, patients with atopic asthma and healthy subjects when challenged with exacerbating factors in vitro: lipopolysaccharide (LPS), house dust mite (HDM) and cat allergen. <b><i>Methods:</i></b> PBMC isolated from patients with severe atopic asthma and COPD, as well as healthy subjects were stimulated with rDer p 1 DG, rFel d 1 DG and LPS. The changes in the expression of 47 genes belonging to five groups (phospholipase A₂, eicosanoids, transcription factors, cytokines and airway remodeling) were studied using TaqMan low density array cards. Immunoblotting was used to study relative protein expression. <b><i>Results:</i></b> rDer p 1 significantly up-regulated the expression of <i>PLA2G4A, PLA2G6, PLA2G15, CYSLTR1, LB4R2, PTGS1, PTGS2</i>, <i>FOXP1</i>, <i>GATA3</i>, <i>HDAC2</i>, <i>IREB2</i>, <i>PPARG</i>, <i>STAT4</i>, <i>TSLP </i>and<i> CHI3L1</i> genes in asthmatics in comparison to healthy subjects. LPS induced significant expression of <i>ANXA1</i> and<i> LTA4H </i>in asthmatics when compared to COPD patients and healthy subjects. <i>SOX6,</i><i>STAT4 </i>and <i>IL1RL1 </i>were induced in COPD after LPS stimulation. Analysis of protein expression revealed a pattern similar to mRNA expression. <b><i>Conclusions:</i></b> LPS-induced exacerbation of asthma and COPD is characterized by differential expression of selected genes in PBMC. HDM allergen changed the expression profile of inflammatory genes between patients with asthma of atopic origin and healthy controls.</p