Taking turns at talk on screen : an analysis of the correlation between words and images in cinematic discourse

博士(文学)神戸市外国語大

モノクローナルIgAの作製とその利用

We hypothesize that IgA immune complex in mother's milk functions for the prevention of food allergy through oral immune tolerance. To prove this hypothesis and to establish the allergic cure by using IgA immune complex as vaccine, a mass preparation of mouse IgA was performed. We established monoclonal antibody producing hybridomas with mesentery lymph nodes and got three kinds of monoclonal IgA. Their Light chains were all κ and the molecular weight was about 280 kDa, suggesting their dimmer form. The antigen specificity of these antibodies was unidentified, but they did not react with major food allergens. Monoclonal IgA was effectively purified with a Protein L column from the culture supernatant of the hybridoma. Ovalbumin was coupled to the monoclonal IgA with a hetero-bifunctional crosslinking reagent, Sulfosuccinimidyl 6- [3' (2-pyridyldithio) -propionamido] hexanoate. Formation of the pseudo IgA immune complex was confirmed by a sandwich type of Enzyme Linked Immunosorbent Assay with the anti-ovalbumin antibody and the anti-mouse IgA (α) antibod

ヒト外分泌液中の食品タンパク質特異的IgAおよびその免疫複合体

We have revealed the occurrence of food proteins as immune complexes in human breast milk. In this report, we aimed to establish the generality of the fact in human exocrine fluids by analyzing food proteinspecific IgAs and their immune complexes in human saliva and tear. Food protein-specific IgAs and their immune complexes were able to be determined in human saliva and tear. The levels were high in order of nursing mothers, adults, and infants. There was no correlation between total IgA and specific IgAs. On the other hand, negative correlation between serum IgE and salivary IgA specific to egg white proteins was observed in infants with atopic eczema. Then, it was suggested that the degree of maturation of mucosal immunity in individual person could be monitored as the anti-allergic parameter by analyzing food protein-specific IgAs in saliva. Saliva can be easily prepared without pain and prick. Utility of saliva as a biological material for clinical diagnosis and physiological significance of the food protein-IgA immune complexes in exocrine fluids were propose

ヒトIgA に対するイムノクロマトグラフィーの開発

Immunochromatography (ICG) strip test based on monoclonal antibodies (mAbs) conjugated with gold nanoparticles was developed and its application for primary screening of IgA in human saliva was evaluated. Six mAbs were established against human secretory IgA. anocolloidal gold as the detection reagent was labelled with mAb ④. MAb ⑥ was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. After reaction of mAb ④-nanocolloidal gold probe with IgA in saliva, resulting immune complex was vertically developed, captured by mAb ⑥ immobilized on the membrane and visualized as a gold band. In the optimized investigational conditions, the ICG strip test could distinguish human secretory IgA in the range from 1 to 20 μg/mL and was sufficiently sensitive to find out incomplete IgA deficiency from salivary screening. It took only 10 minutes to accomplish a detection of salivary IgA in this assay, compared to 5 hours by sandwich ELISA

Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein

Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer

Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: A potential novel strategy to enhance zinc absorption by ZIP4 targeting

Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased bothmZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells.Our results suggest that ZIP4 targetingmay represent a new strategy to improve zinc absorption in humans