Not Available

Not AvailableAquaculture of shrimps is an important farming system in the coastal areas of India. It has been significantly contributing for the rural development through employment generation, income, livelihood development and discouragement of migration to urban areas, to some extent. Additionally, through exports it provides a sum of Rs.6,000 crores to the national economy. Tiger shrimp (Penaeus monodon), an indigenous shrimp, has been the only candidate shrimp species cultured by our farmers till 2009. However, due to mismanagements at hatchery and farm levels a formidable viral disease entered into our water bodies to the extent that most of our natural shrimp stock has been found infected. The sector was in crisis and this led to better management practices for seed production and farming which were developed and popularised among the farmers. The farmers also realised that quality seed would minimise the disease risk by 50% and majority of them followed good shrimp aquaculture practices. Nevertheless, persistence of white spot disease and continued havocs, diverted global shrimp aquaculture towards Pacific white shrimp (Penaeus vannamei) due to availability of its Specific Pathogen Free (SPF) broodstock and market scenario. Reacting to the eagerness of the farmers to adopt high volume shrimp aquaculture, the Government of India has introduced the exotic Pacific white shrimp (Penaeus vannamei) for commercial production in 2009. As expected, the Pvannamei has revived Indian shrimp farming to its pre-1995 status with an estimated production of 60,000 tonnes from about 5000 ha of registered farms in 2011-12.Not Availabl

Quantitative Proteomics Analysis of Camelina sativa Seeds Overexpressing the <i>AGG3</i> Gene to Identify the Proteomic Basis of Increased Yield and Stress Tolerance

Camelina sativa, a close relative of Arabidopsis, is an oilseed plant that is emerging as an important biofuel resource. The genome and transcriptome maps of Camelina have become available recently, but its proteome composition remained unexplored. A labeling LC-based quantitative proteomics approach was applied to decipher the Camelina seed proteome, which led to the identification of 1532 proteins. In addition, the effect of overexpression of the Arabidopsis G-protein γ subunit 3 (<i>AGG3</i>) on the Camelina seed proteome was elucidated to identify the proteomic basis of its increased seed size and improved stress tolerance. The comparative analysis showed a significantly higher expression of proteins involved in primary and secondary metabolism, nucleic acid and protein metabolism, and abscisic acid related responses, corroborating the physiological effects of <i>AGG3</i> overexpression. More importantly, the proteomic data suggested involvement of the AGG3 protein in the regulation of oxidative stress and heavy metal stress tolerance. These observations were confirmed by the physiological and biochemical characterization of <i>AGG3</i>-overexpressing seeds, which exhibit a higher tolerance to exogenous cadmium in a glutathione-dependent manner. The activity of multiple redox-regulating enzymes is higher in seeds expressing enhanced levels of <i>AGG3</i>. Overall, these data provide critical evidence for the role of redox regulation by the AGG3 protein in mediating important seed-related traits