ADMET Investigations On A Synthetic Derivative Of Genistein, And Molecular Docking Experiments Targeting Estrogen Receptor-α (ER-α) In The Pancreas

The main goal of the current research was to perform ADMET and molecular docking studies for a synthetic genistein derivative that can imitate Estrogen and function as an endocrine disruptor, activating the ER receptor on beta-cells in the pancreas to release insulin. The created molecule was molecularly docked using the online molecular docking research tool Dockthor. NGL viewer, an online program for viewing Dockthor data, displayed the docking experiment results. The 2D legend-protein interactions were estimated with BIOVIA Discovery Studio Visualizer. Estrogen-Receptor Alpha was the targeted target, while Compound-A was employed as the legend. In this study, we created a synthetic derivative of genistein, an analogue of Estrogen in terms of ER-α receptor binding. We used molecular docking to evaluate the affinity of compound-A binding to the ER-α and its 2D interactions and Ramachandran plots. We then ran ADMET experiments on the molecule, which revealed a substantial relationship with the molecule's Estrogen Receptor binding capabilities, as well as scores for absorption, distribution, metabolism, excretion, and toxicity

Capstrum Coefficient Features Analysis for Multilingual Speaker Identification System

The Capstrum coefficient features analysis plays a crucial role in the overall performance of the multilingual speaker identification system. The objective of the research work to investigates the results that can be obtained when you combine Mel-Frequency Cepstral Coefficients (MFCC) and Gammatone Frequency Cepstral Coefficients (GFCC) as feature components for the front-end processing of a multilingual speaker identification system. The MFCC and GFCC feature components combined are suggested to improve the reliability of a multilingual speaker identification system. The GFCC features in recent studies have shown very good robustness against noise and acoustic change. The main idea is to integrate MFCC & GFCC features to improve the overall multilingual speaker identification system performance. The experiment carried out on recently collected multilingual speaker speech database to analysis of GFCC and MFCC. The speech database consists of speech data recorded from 100 speakers including male and female. The speech samples are collected in three different languages Hindi, Marathi and Rajasthani. The extracted features of the speech signals of multiple languages are observed. The results provide an empirical comparison of the MFCC-GFCC combined features and the individual counterparts. The average language-independent multilingual speaker identification rate 84.66% (using MFCC), 93.22% (using GFCC)and 94.77% (using combined features)has been achieved

Multilingual Speaker Identification using analysis of Pitch and Formant frequencies

In the modern digital automated world, speaker identification system plays a very important role in the field of fast growing internet based communications. In India there are many people who are bi-lingual or multilingual, so the requirements to design such system which is used to identify the multilingual speakers. Present paper explores the idea to identify multi-lingual person by basic features. For this the speech signals of three indian languages i.e Hindi, Marathi and Rajasthani are recorded and basic features pitch, first three formant frequency calculated from PRAAT software. The observation has been presented that the pitch and first three formant frequencies F1,F2 and F3 of speaker are increases when speaker change the language from rajasthani to hindi to marathi. The percentage deviation in pitch as well as formant frequencies for Rajasthani and Marathi from hindi are positive and negative respectively for utterance “p”. Similar analysis has been perform for ’k aand >. This observation will help to make such system which is used to identify the speaker in multilingual environments

A novel approach to track public emotions related to epidemics in multilingual data

Emergence of new epidemic and re-appearance of older diseases causes great impact towards public health. Surveys based techniques which are costly and time-consuming are the most popular methods to measure information related to public health and used in decision making. Early monitoring of these epidemics helps in rapid decision making. Social media platforms provide rich source of information related to public health in forms of blogs, tweets, public posts etc., but these data is in unstructured form contains multiple languages words. This research focused on developing an automatic system for detecting public emotions related to epidemics in multilingual unstructured data to gain deeper understanding of public emotions and health related information. This approach gives timely information related to epidemics, corresponding symptoms, prevention techniques and awareness, which can help government and health agencies for rapid decision making. Experimental analysis of data set provides results that significantly beat the baseline term counting methods used for sentiment analysis

Monocrotophos Induced Apoptosis in PC12 Cells: Role of Xenobiotic Metabolizing Cytochrome P450s

Monocrotophos (MCP) is a widely used organophosphate (OP) pesticide. We studied apoptotic changes and their correlation with expression of selected cytochrome P450s (CYPs) in PC12 cells exposed to MCP. A significant induction in reactive oxygen species (ROS) and decrease in glutathione (GSH) levels were observed in cells exposed to MCP. Following the exposure of PC12 cells to MCP (10−5 M), the levels of protein and mRNA expressions of caspase-3/9, Bax, Bcl2, P53, P21, GSTP1-1 were significantly upregulated, whereas the levels of Bclw, Mcl1 were downregulated. A significant induction in the expression of CYP1A1/1A2, 2B1/2B2, 2E1 was also observed in PC12 cells exposed to MCP (10−5 M), whereas induction of CYPs was insignificant in cells exposed to 10−6 M concentration of MCP. We believe that this is the first report showing altered expressions of selected CYPs in MCP-induced apoptosis in PC12 cells. These apoptotic changes were mitochondria mediated and regulated by caspase cascade. Our data confirm the involvement of specific CYPs in MCP-induced apoptosis in PC12 cells and also identifies possible cellular and molecular mechanisms of organophosphate pesticide-induced apoptosis in neuronal cells

Hirayama disease: a rare neurological condition of cervical cord pathology

The disease is characterized by a progressive muscle weakness and wasting of distal upper limb muscles sparing brachioradialis. The pathology is in the lower cervical cord due to forward displacement of dural sac and spinal cord by neck flexion. Repeated neck flexion may result in ischemia of the lower cervical anterior horns. The diagnosis can be confirmed by MRI, EMG. It requires a high degree of suspicion in a young patient presenting with unilateral upper limb distal muscle weakness with no sensory involvement, as early diagnosis will help in preventing the progression of the disease by employing simple measures like usage of a cervical collar. We report a case of a 25 year old man presenting with progressive distal upper limb muscle wasting, on investigating further diagnosed to have Hirayama disease

Effect of Multiple Rounds of Mass Drug Administration under Lymphatic Filariasis Elimination Program on Prevalence of Soil-Transmitted Helminthiasis in One of the Co-endemic Districts of India

Both soil-transmitted helminthiasis and lymphatic filariasis are co-endemic in some districts of India. The study was aimed at comparing the prevalence of soil-transmitted helminthiasis (STH) infection before and after mass drug administration (MDA) given for lymphatic filariasis at Alappuzha.We examined stool samples of 219 children aged between 9 and 10 years from nine schools at Alappuzha, Kerala, in June 2015. The current STH situation was compared with that in the year 1999 (before the institution of MDA) in the same age group in the same district. WHO-recommended Kato-Katz technique was used for STH prevalence estimation. Information regarding socio-demographic variables, sanitation, personal hygiene practices was also recorded. Chi-square test was used to study association of risk factors with STH infection.A total of 79 (36.07%) children were found to be positive for STH infection. Out of all samples positive for Ascaris lumbricoides, 4 (5%) were found co-infected with Trichuris trichiura and none with hookworm. Factors like absence of household latrine, lack of hand washing before meals and practice of consumingunwashed fruits were found to be significantly associated with occurrence of STH infection.Over the years, consecutive rounds of MDA for LF have led to a decline in intestinal worm burden but with little effect on the overall prevalence of STH. Thus, STH control activities working synergistically with the Filarial Elimination Strategy can yield better results in the co-endemic areas with optimum resourceutilization

Functional outcome of hybrid external fixator in proximal tibial fractures Schatzker type V and VI with Gustillo grade-II

Background: Management of high energy tibial plateau fractures along with extensive soft tissue damage is still challenging to many orthopaedic surgeons. This study evaluates the purpose of hybrid external fixator intreating high energy tibial plateau fractures with minimal invasion and accurate reduction.Methods: Twenty patients with high energy Schatzker type V and VI tibial plateau fractures with severe soft tissue injury were enrolled into the study in RNT medical college, Udaipur.Results: The results- bony union, range of movements and associated complications were assessed. All fractures united in an average time period of 20 weeks. Ten patients developed knee stiffness, five patients developed delayed union andthreenon-union.15 patients required split skin graft. Final outcome showed excellent score in 53 patients.Conclusions: Hybrid external fixation is a safe option for managing complex high energy tibial plateau fractures by simultaneously providing adequate fracture stabilization and necessary protection to soft tissue healing to achieve bony union

Comparative Genomics Reveals the High Copy Number Variation of a Retro Transposon in Different Magnaporthe Isolates

Magnaporthe oryzae is one of the fungal pathogens of rice which results in heavy yield losses worldwide. Understanding the genomic structure of M. oryzae is essential for appropriate deployment of the blast resistance in rice crop improvement programs. In this study we sequenced two M. oryzae isolates, RML-29 (avirulent) and RP-2421 (highly virulent) and performed comparative study along with three publically available genomes of 70-15, P131, and Y34. We identified several candidate effectors (>600) and isolate specific sequences from RML-29 and RP-2421, while a core set of 10013 single copy orthologs were found among the isolates. Pan-genome analysis showed extensive presence and absence variations (PAVs). We identified isolate-specific genes across 12 isolates using the pan-genome information. Repeat analysis was separately performed for each of the 15 isolates. This analysis revealed ∼25 times higher copy number of short interspersed nuclear elements (SINE) in virulent than avirulent isolate. We conclude that the extensive PAVs and occurrence of SINE throughout the genome could be one of the major mechanisms by which pathogenic variability is emerging in M. oryzae isolates. The knowledge gained in this comparative genome study can provide understandings about the fungal genome variations in different hosts and environmental conditions, and it will provide resources to effectively manage this important disease of rice

Determination of Nifedipine by Validated RP-HPLC Method in Bulk and Pharmaceutical Dosage Form

The present paper deals with the development and validation of reverse phase HPLC method for the determination of Nifedipine on Nucleosil 100, 5 μm, C8, 250 x 4.0 mm column. A mobile phase consisting of 40 ml 2-propanol: 60 ml phosphoric acid 0.85% was employed in this study. The flow rate was kept at 0.8 ml/min and the injection volume was 10 µl. The separation was performed at 40°C. Eluents were monitored by UV detector set at 237 nm. The developed method was statistically validated for the linearity, precision, robustness, specificity and solution stability. The specificity of the method was ascertained by force degradation studies by acid and alkali hydrolysis, oxidation, heat and photo degradation. The degraded products were well resolved from the analyte peak with significant differences in their retention time values