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Not AvailableVibrio parahaemolyticus is known to cause disease and infection in humans. This study was conducted to understand the prevalence of V. parahaemolyticus and the presence of virulence factors in environmental isolates of V. parahaemolyticus, collected from three major shrimp producing states viz, West Bengal, Andhra Pradesh and Gujarat of India during February 2014 to June 2017. Total 183 V. parahaemolyticus were isolated from moribund shrimp and identified using toxR and 16S rRNA gene. Polymerase Chain Reaction (PCR) was performed to detect virulence genes under T3SS1 and T3SS2 along with AHPND. All the isolates showed presence of vcrD1, vp1680 and vopD1 gene under T3SS1 but negative for AHPND. Only 3% of isolates showed presence of all the virulence genes (vcrD2, vopD2, vopB2 and vopC) used in the present study under T3SS2. The isolates were also tested for the presence of tdh, trh or both the genes; 8% of the isolates had trh gene and 3% of the isolates had tdh gene. The genetic diversity within and between V. parahaemolyticus isolated from three different geographical locations, was analyzed by using 16S rRNA gene. Though the biotype diversity (h) was high for all three populations, phylogenetic study of V. phaemolyticus revealed an admixture of biotypes. Tajima's D (−2.59470; p < .001) test of selective neutrality and Fu's Fs (−32.422; p < .001) test were negative and significant, suggesting a sudden population expansion with limited time for population differentiation.Not Availabl

Comparative Study on the Outcome of Primary Skin Closure Versus Delayed Primary Skin Closure in Case of Emergency Laparotomies

Introduction: One of the most common complications that one can encounter is the surgical site infection (SSI) after any surgical procedures especially after exploratory laparotomy for perforative peritonitis. A surgical wound is considered as infected when there is purulent drainage from the wound. The purpose of the present study was to compare the primary wound closure technique and delayed primary wound closure technique with regard to the rate of wound infection and other complications which are usually associated with the infection like wound dehiscence, stitch sinuses, incisional hernias and duration of hospital stay. Materials and Methodology: 120 participants were randomly divided into two groups with 60 subjects each for primary wound closure and delayed primary wound closure. Data was coded and entered in Microsoft Excel sheet and the data analysis was done using SPSS-17. Association between qualitative variables will be analysed using Chi-square test. Associations between quantitative variables were analysed using independent sample t-test. Non parametric tests were if whenever necessary. When a p value of less than 0.05 is considered as statistically significant.&nbsp

Characterization of natural fiber extracted from Bauhinia vahlii bast subjected to different surface treatments: A potential reinforcement in polymer composite

Bauhinia vahlii (BV) is a great source of cellulosic biomass, and it is gaining popularity as a renewable resource. BV bast fiber is proposed to have the potential to replace synthetic fibers in biopolymer composites as a reinforcing material. This investigation deals with the extraction, surface modification and comprehensive characterization of BV bast fibers which could be used for the production of sustainable fiber-reinforced polymer composites. The extracted fibers were chemically treated with sodium hydroxide, sodium chlorite, and benzoyl chloride. Then, the chemical properties, mechanical properties, surface morphology and thermal properties were investigated. An improvement in chemical and mechanical properties was observed after surface modification of fibers. Benzoylation treated BV bast fibers revealed highest tensile strength of 128.56 MPa and Young’s modulus of 8.34 GPa. In addition, after treatment, the fibers had rougher surface as seen from SEM images. The surface treatments removed a specific quantity of hemicelluloses, lignin, and pectin from the natural fiber surface, according to FTIR analysis. The surface treatments had a good impact on the crystallinity index of the natural fibers, according to XRD analysis. The characterization results confirmed that BV bast fibers could be used for the production of sustainable fiber reinforced polymer composites