129 research outputs found
Peripubertal high-fat diet promotes c-Myc stabilization in mammary gland epithelium
Dietary fat consumption during accelerated stages of mammary gland development, such as peripubertal maturation or pregnancy, is known to increase the risk for breast cancer. However, the underlying molecular mechanisms are not fully understood. Here we examined the gene expression profile of mouse mammary epithelial cells (MMECs) on exposure to a high-fat diet (HFD) or control diet (CD). Trp53-/- female mice were fed with the experimental diets for 5Â weeks during the peripubertal period (3-8Â weeks of age). The treatment showed no significant difference in body weight between the HFD-fed mice and CD-fed mice. However, gene set enrichment analysis predicted a significant enrichment of c-Myc target genes in animals fed HFD. Furthermore, we detected enhanced activity and stabilization of c-Myc protein in MMECs exposed to a HFD. This was accompanied by augmented c-Myc phosphorylation at S62 with a concomitant increase in ERK phosphorylation. Moreover, MMECs derived from HFD-fed Trp53-/- mouse showed increased colony- and sphere-forming potential that was dependent on c-Myc. Further, oleic acid, a major fatty acid constituent of the HFD, and TAK-875, an agonist to G protein-coupled receptor 40 (a receptor for oleic acid), enhanced c-Myc stabilization and MMEC proliferation. Overall, our data indicate that HFD influences MMECs by stabilizing an oncoprotein, pointing to a novel mechanism underlying dietary fat-mediated mammary carcinogenesis.Nilakshi Kulathunga, Susumu Kohno, Paing Linn, Yuuki Nishimoto, Shinâichi Horike ... Sharad Kumar ... et al
Elliptic flow of electrons from heavy-flavor hadron decays in Au+Au collisions at 200, 62.4, and 39 GeV
We present measurements of elliptic flow () of electrons from the decays
of heavy-flavor hadrons () by the STAR experiment. For Au+Au collisions
at 200 GeV we report , for transverse momentum
() between 0.2 and 7 GeV/c using three methods: the event plane method
({EP}), two-particle correlations ({2}), and four-particle
correlations ({4}). For Au+Au collisions at = 62.4 and
39 GeV we report {2} for GeV/c. {2} and {4} are
non-zero at low and intermediate at 200 GeV, and {2} is consistent
with zero at low at other energies. The {2} at the two lower beam
energies is systematically lower than at 200 GeV for
GeV/c. This difference may suggest that charm quarks interact less
strongly with the surrounding nuclear matter at those two lower energies
compared to GeV.Comment: Version accepted by PR
Measurement of the mass difference and the binding energy of the hypertriton and antihypertriton
According to the CPT theorem, which states that the combined operation of
charge conjugation, parity transformation and time reversal must be conserved,
particles and their antiparticles should have the same mass and lifetime but
opposite charge and magnetic moment. Here, we test CPT symmetry in a nucleus
containing a strange quark, more specifically in the hypertriton. This
hypernucleus is the lightest one yet discovered and consists of a proton, a
neutron, and a hyperon. With data recorded by the STAR
detector{\cite{TPC,HFT,TOF}} at the Relativistic Heavy Ion Collider, we measure
the hyperon binding energy for the hypertriton, and
find that it differs from the widely used value{\cite{B_1973}} and from
predictions{\cite{2019_weak, 1995_weak, 2002_weak, 2014_weak}}, where the
hypertriton is treated as a weakly bound system. Our results place stringent
constraints on the hyperon-nucleon interaction{\cite{Hammer2002,
STAR-antiH3L}}, and have implications for understanding neutron star interiors,
where strange matter may be present{\cite{Chatterjee2016}}. A precise
comparison of the masses of the hypertriton and the antihypertriton allows us
to test CPT symmetry in a nucleus with strangeness for the first time, and we
observe no deviation from the expected exact symmetry
- âŠ