Adenosine deaminase enzyme activity is increased and negatively correlates with catalase, superoxide dismutase and glutathione peroxidase in patients with Behçet's disease: original contributions/clinical and laboratory investigations.

AIM: Behçet's disease (BD) is an inflammatory vasculitis with immunologic, endothelial and neutrophil alterations. Adenosine deaminase (AD) is a marker of T-cell activation and is related to the production of reactive oxygen species by neutrophils with the production of NO(*), O(2)(*-), H(2)O(2) and OH(*). We reported increased tumour necrosis factor-alpha, soluble interleukin-2 receptor, interleukin-6, interleukin-8 and NO(*) in active BD. As there is a relation between cytokines, T cells and oxidative stress in inflammatory diseases, this study further evaluated: (1) plasma AD activity and its correlation with acute phase reactants; (2) thiobarbituric acid-reactive substances (TBARS) as an indicator for lipid peroxidation; and (3) antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSHPx) and catalase in patients with BD. The effect of disease activity and correlations between the measured parameters were explored. METHODS: A total of 35 active (n=17) or inactive (n=18) patients with BD (16 men, 19 women) satisfying International Study Group criteria, and 20 age-matched and sex-matched controls (nine men, 11 women) were included in this cross-sectional case-control study. AD and TBARS were measured in plasma, catalase in red blood cells (RBC), and SOD and GSHPx in both plasma and RBC in both groups. Acute phase reactants (alpha(1)-antitrypsin, alpha(2)-macroglobulin, neutrophils, erythrocyte sedimentation rate) were used to classify patients as active or inactive. RESULTS: Plasma AD (mean+/-standard error of the mean, 36.1+/-0.7 U/l) and TBARS (4.2+/-0.1 nmol/ml) levels were significantly (for each, p<0.001) higher in BD than in controls (24.1+/-0.8 U/l and 1.6+/-0.1 nmol/ml, respectively). RBC catalase activity was significantly (p<0.001) lower in BD than in controls (120.9+/-3.8 versus 160.3+/-4.1 k/g haemoglobin). SOD and GSHPx activities were significantly lower in both plasma and erythrocytes of patients with BD than in controls (plasma SOD, 442.4+/-8.6 versus 636.4+/-9.2 U/ml, p<0.001; RBC SOD, 3719.2+/-66.0 versus 4849.7+/-49.0 U/g haemoglobin, p<0.001; plasma GSHPx, 73.1+/-1.5 versus 90.6+/-2.9 U/ml, p<0.001; RBC GSHPx, 600.7+/-8.0 versus 670.6+/-10.1 U/g haemoglobin, p<0.001). Active BD patients had significantly lower antioxidant enzymes (except RBC catalase) and higher AD and TBARS levels than inactive subjects (for each, p<0.01). When considering all BD patients, a significant positive correlation was present between AD and TBARS (p<0.001) whereas both AD and TBARS were negatively correlated with antioxidant enzymes (for each, p<0.05). CONCLUSIONS: AD and lipid peroxidation are increased and associated with defective antioxidants in BD, suggesting interactions between activated T cells and neutrophil hyperfunction. Measures of pro-oxidative stress and antioxidative defence with AD activity as an indicator of T-cell activation can be considered as significant supportive diagnostic indicators, especially in active disease. In addition, strengthening the antioxidant defence may contribute to treatment modalities

Göz ve Orbita Yapılarının Konjenital Anomalileri

Göz küresi ve içinde yerleştiği orbita ve eklerinin konjenital anomalileri nadir olmayarak görülen bozukluklardır. İzole bir sporadik anomali olarak veya bir sendromun parçası olarak görülebilirler. Vücudun diğer organ ve doku anomalilerinde olduğu gibi genel teratojenik etkenler burada da söz konusudur. Herediter özellikler büyük oranda etkilidirler. Anomaliye yol açan faktörün etki etmeye başladığı intrauterin döneme bağlı olarak anomalinin ağırlığı değişir. Patolojik tablo tek ya da iki taraflı, simetrik ya da asimetrik olabilir

Our results of pars plana vitrectomy in patients with proliferative diabetic retinopathy Proli̇ferati̇f di̇abeti̇k reti̇nopati̇li̇ olgularda pars plana vi̇trektomi̇ sonuçlarimiz

Purpose: To evaluate the results and complications of pars plana vitrectomy (PPV) in patients with proliferative diabetic retinopathy (PDR). Material and Methods: Sixty-five eyes of 53 patients with a mean age of 53.5±13.6 years who had undergone PPV for treatment of PDR complications were included in the study. In all eyes, posterior hyaloid and preretinal membranes were removed after standard PPV. Pre- and postoperative visual acuities, anterior and posterior segment findings, follow-up time and encountered complications were recorded. Results: Mean follow-up time was 13.6±9.2 months. Anatomical success was achieved in 94.4% of patients with vitreous hemorrhage, and in 83.3% of patients with fractional retinal detachment. Postoperatively, visual acuity improved in 81.5% of eyes, remained stable or decreased in 18.4% of eyes. The most common intraoperative complications were retinal microhemorrhages and formation of iatrogenic retinal breaks. Transient intraocular pressure (IOP) elevation and re-hemorrhages were the most common complications in the early and cataract formation in the late postoperative period. Conclusion: Pars plana vitrectomy is very effective in the treatment of PDR complications, but these patients should be closely followed up for possible complications