A new stand of Hieracium pilosum Schleich. ex Froelich (Asteraceae) in the Polish Tatra Mountains

A new stand of H. pilosum, a species not reported before from the territory of Poland, was found in the Polish Western Tatra Mts. It grows in numerous stands on limestone rocks on the slopes of Upłaziańska Kopka, at ca. 1400 m a.s.l. Moreover, typical form of this species, collected in the Polish Western Tatra Mts, was also found in herbaria of KRA and KRAM. These data indicate that H. pilosum is a widespread species in the described region but it was unrecognized from H. villosum so far

Cyanobacteria use both p-hydroxybenozate and homogentisate as a precursor of plastoquinone head group

Until recently it was believed that cyanobacterial pathway of plastoquinone biosynthesis is analogical to that of higher plants. In plants, homogentisate is a precursor of the hydrophilic head group of plastoquinone. Recent experiments on Synechocystis sp. PCC 6803 have shown that this organism takes advantage of another pathway that resembles ubiquinone biosynthetic pathway of α-, β- and γ-proteobacteria. In the present work, we have analysed the content of plastoquinone, tocopherol and tocopherolquinone in six strains of cyanobacteria and compared the obtained results with search for genes of homologues of enzymes participating in tocopherol and ubiquinone biosynthesis. We have shown that inhibition of homogentisate synthesis lowers tocopherol content but does not affect plastoquinone synthesis in Synechococcus sp. PCC 7002. Inhibitors of p-hydroxybenzoate and homogentisate prenyltransferases selectively influenced plastoquinone and tocopherol biosynthesis in Synechocystis sp. PCC 6803. Radiolabelled 14C-p-hydroxybenzoate was incorporated into plastoquinone by three cyanobacteria species investigated. Although, when ^14C-homogentisate was added to growth medium, the labelled plastoquinone was found in extracts of the cyanobacteria. Synechocystis sp. PCC 6803 grown in the presence of ^14C-homogentisate showed also small amounts of the labelled tyrosine, suggesting that cyanobacteria are able to incorporate exogenously added homogentisate into shikimate pathway. Keyword

Activity of tocopherol oxidase in Phaseolus coccineus seedlings

In the present study, we have demonstrated that membrane-free extracts of etiolated shoots of Phaseolus coccineus seedlings show tocopherol oxidase activity. For this reaction, presence of membrane lipids, such as lecithin and mixture of plant lipids was required. The rate of the reaction was the highest for α-tocopherol and decreased in the order α ≫ β > γ > δ tocopherols. In the case of α-tocopherol, the main oxidation product was α-tocopherolquinone, while for the other tocopherol homologues the dominant products were other derivatives. When the enzyme activity was measured in leaves, hypocotyls and roots of etiolated seedlings of P. coccineus, the oxidase activity was the highest in extracts of leaves and decreased towards the roots where no activity was detected. The effect of hydrogen peroxide and of different inhibitors on the reaction suggest that tocopherol oxidase does not belong to peroxidases or flavin oxidases but rather to multi-copper oxidases, such as polyphenol oxidases or laccases. On the other hand, catechol, the well-known substrate of polyphenol oxidases and laccases, was not oxidized by the enzyme, indicating a high substrate specificity of the tocopherol oxidase

Occurrence of neoxanthin and lutein epoxide cycle in parasitic Cuscuta species

In the present study, xanthophyll composition of eight parasitic Cuscuta species under different light conditions was investigated. Neoxanthin was not detected in four of the eight species examined, while in others it occurred at the level of several percent of total xanthophylls. In C. gronovii and C. lupuliformis it was additionally found that the neoxanthin content was considerably stimulated by strong light. In dark-adapted plants, lutein epoxide level amounted to 10-22% of total xanthophylls in only three species, the highest being for C. lupuliformis, while in others it was below 3%, indicating that the lutein epoxide cycle is limited to only certain Cuscuta species. The obtained data also indicate that the presence of the lutein epoxide cycle and of neoxanthin is independent and variable among the Cuscuta species. The xanthophyll cycle carotenoids violaxanthin, antheraxanthin and zeaxanthin were identified in all the examined species and occurred at the level found in other higher plants. The xanthophyll and lutein epoxide cycle pigments showed typical response to high light stress. The obtained results also suggest that the ability of higher plants to synthesize lutein epoxide probably does not depend on the substrate specificity of zeaxanthin epoxidase but on the availability of lutein for the enzyme

Molecular analysis of Sorbus Sp. from the Pieniny Mts. and its relation to other Sorbus Species

The systematic position of Sorbus population occurring in the Pieniny Mts. is controversial. To verify its taxonomic status we studied the ITS sequence of closely related species of the S. aria group: Sorbus sp. from the Pieniny Mts., S. aria from the Tatra Mts., S. graeca from the Balkans, and other well-distinguished native Polish Sorbus species (S. aria, S. aucuparia, S. intermedia and S. torminalis). As a reference we examined Sorbus populations closest to the Pieniny Mts. where S. graeca was reported to occur, in Slovakia. The results indicate that the Sorbus plants found in the Pieniny Mts. differ genetically from those in the Tatra Mts. but are identical to those collected from the Vihorlat Mts. in Slovakia and are closely related to S. graeca from the Balkan

Salinity-induced changes in plastoquinone pool redox state in halophytic Mesembryanthemum crystallinum L.

We have analyzed the effect of salinity on photosystem II (PSII) photochemistry and plastoquinone (PQ) pool in halophytic Mesembryanthemum crystallinum plants. Under prolonged salinity conditions (7 or 10 days of 0.4 M NaCl treatment) we noted an enlarged pool of open PSII reaction centers and increased energy conservation efficiency, as envisaged by parameters of the fast and slow kinetics of chlorophyll a fluorescence. Measurements of oxygen evolution, using 2,6-dichloro-1,4-benzoquinone as an electron acceptor, showed stimulation of the PSII activity due to salinity. In salt-acclimated plants (10 days of NaCl treatment), the improved PSII performance was associated with an increase in the size of the photochemically active PQ pool and the extent of its reduction. This was accompanied by a rise in the $NADP^{+}$/NADPH ratio. The presented data suggest that a redistribution of PQ molecules between photochemically active and non-active fractions and a change of the redox state of the photochemically active PQ pool indicate and regulate the acclimation of the photosynthetic apparatus to salinity

Enhanced chloroplastic generation of H_{2}O_{2} in stress-resistant Thellungiella salsuginea in comparison to Arabidopsis thaliana

In order to find some basis of salinity resistance in the chloroplastic metabolism, a halophytic Thellungiella salsuginea was compared with glycophytic Arabidopsis thaliana. In control T.s. plants the increased ratios of chlorophyll a/b and of fluorescence emission at 77 K (F_{730}/F_{685}) were documented, in comparison to A.t.. This was accompanied by a higher YII and lower NPQ (non-photochemical quenching) values, and by a more active PSI (photosystem I). Another prominent feature of the photosynthetic electron transport (PET) in T.s. was the intensive production of H_2O_2 from PQ (plastoquinone) pool. Salinity treatment (0.15 and 0.30 M NaCl for A.t. and T.s., respectively) led to a decrease in ratios of chl a/b and F_{730}/F_{685}. In A.t., a salinity-driven enhancement of YII and NPQ was found, in association with the stimulation of H_2O_2 production from PQ pool. In contrast, in salinity-treated T.s., these variables were similar as in controls. The intensive H_2O_2 generation was accompanied by a high activity of PTOX (plastid terminal oxidase), whilst inhibition of this enzyme led to an increased H_2O_2 formation. It is hypothesized, that the intensive H_2O_2 generation from PQ pool might be an important element of stress preparedness in Thellungiella plants. In control T.s. plants, a higher activation state of carboxylase ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) was also documented in concert with the attachment of Rubisco activase (RCA) to the thylakoid membranes. It is supposed, that a closer contact of RCA with PSI in T.s. enables a more efficient Rubisco activation than in A.t