Implementation of a model for identifying Essentially Derived Varieties in vegetatively propagated Calluna vulgaris varieties

<p>Abstract</p> <p>Background</p> <p>Variety protection is of high relevance for the horticultural community and juridical cases have become more frequent in a globalized economy due to essential derivation of varieties. This applies equally to <it>Calluna vulgaris</it>, a vegetatively propagated species from the <it>Ericaceae </it>family that belongs to the top-selling pot plants in Europe. We therefore analyzed the genetic diversity of 74 selected varieties and genotypes of <it>C. vulgaris </it>and 3 of <it>Erica </it>spp. by means of RAPD and iSSR fingerprinting using 168 mono- and polymorphisms. The same data set was utilized to generate a system to reliably identify Essentially Derived Varieties (EDVs) in <it>C. vulgaris</it>, which was adapted from a method suggested for lettuce and barley. This system was developed, validated and used for selected tests of interest in <it>C. vulgaris</it>.</p> <p>Results</p> <p>As expected following personal communications with breeders, a very small genetic diversity became evident within <it>C. vulgaris </it>when investigated using our molecular methods. Thus, a dendrogram-based assay to detect Essentially Derived Varieties in this species is not suitable, although varieties are propagated vegetatively. In contrast, the system applied in lettuce, which itself applies pairwise comparisons using appropriate reference sets, proved functional with this species.</p> <p>Conclusion</p> <p>The narrow gene pool detected in <it>C. vulgaris </it>may be the genetic basis for juridical conflicts between breeders. We successfully tested a methodology for identification of Essentially Derived Varieties in highly identical <it>C. vulgaris </it>genotypes and recommend this for future proof of essential derivation in <it>C. vulgaris </it>and other vegetatively propagated crops.</p

Injectable autologous chondrocyte implantation (ACI) in acetabular cartilage defects—three-year results

To evaluate the clinical outcome after arthroscopic matrix-associated injectable autologous chondrocyte implantation (ACI) in patients with large full-thickness acetabular cartilage defects. ACI was performed in young patients with full-thickness acetabular cartilage defects >= 2 cm(2) in a two-step arthroscopic procedure. The patients were followed closely with clinical examinations and pre- and postoperative scores. The modified Harris Hip Score (mHHS), iHOT33 questionnaire (iHOT33) and the Subjective Hip Value (SHV) were surveyed. Demographic patient data was evaluated for influencing factors for the pre- and postoperative results. Thirty-two consecutive cases (4 female, 28 male, mean age 33 years) were included. The average defect size was 4.9 (range: 2-6) cm(2). They were followed at 6, 12, 24 and 36 months postoperatively. Patients had improved significantly from 64 to 91 points (P < 0.001) in the mHHS, from 44% to 86% (P < 0.001) in the iHOT33 and from 54% to 87% (P < 0.001) in the SHV. No surgery related complications were noted. Cell cultivation failed in two cases (7%) and the patients decided for a repeated harvesting of cartilage cylinders followed by a successful ACI. Patients age and size of the cartilage defect showed no significant correlation with the pre- or postoperative results. Injectable ACI is a reliable procedure treating full-thickness acetabular cartilage defects leading to promising results 3 years postoperatively with a significant increase in all scores despite large acetabular cartilage defects in the weight-bearing zone

Correlation of the Subjective Hip Value with Validated Patient-Reported Outcome Measurements for the Hip

Background: The subjective hip value (SHV) was developed as a patient-reported outcome measurement (PROM) that is easily and quickly performed and interpreted. The SHV is defined as a patient's subjective hip measurement tool expressed as a percentage of an entirely normal hip joint, which would score 100%. The hypothesis is that results of the subjective hip value correlate with the results of the modified Harris hip score and the International Hip Outcome Tool in patients with hip-related diseases. Methods: 302 patients completed the modified Harris hip score (mHHS), the International Hip Outcome Tool (iHot-33) as well as the SHV. The SHV consist of only one question: "What is the overall percent value of your hip if a completely normal hip represents 100%?". The patients were divided into five different groups depending on the diagnosis. Pearson correlation was used to evaluate the correlation between the different PROMs and linear regression analysis was used to calculate R2. Results: 302 complete datasets were available for evaluation. There was a high correlation between the SHV and the iHOT-33 (r = 0.847; r2 = 0.692, p < 0.001) and the mHHS (r = 0.832; r2 = 0.717, p < 0.001). The SHV showed a medium (r = 0.653) to high (r = 0.758) correlation with the mHHS and the iHOT-33 in all diagnosis groups. Conclusion: The SHV offers a useful adjunct to established hip outcome measurements, as it is easily and quickly performed and interpreted. The SHV reflects the view of the patient and is independent of the diagnosis. Further research with prospective studies is needed to test the psychometric properties of the score

2-year survival of patients undergoing mild hypothermia treatment after ventricular fibrillation cardiac arrest is significantly improved compared to historical controls

<p>Abstract</p> <p>Background</p> <p>Therapeutic hypothermia has been proven to be effective in improving neurological outcome in patients after cardiac arrest due to ventricular fibrillation (VF). Data concerning the effect of hypothermia treatment on long-term survival however is limited.</p> <p>Materials and methods</p> <p>Clinical and outcome data of 107 consecutive patients undergoing therapeutic hypothermia after cardiac arrest due to VF were compared with 98 historical controls. Neurological outcome was assessed at ICU discharge according to the Pittsburgh cerebral performance category (CPC). A Kaplan-Meier analysis of follow-up data concerning mortality after 24 months as well as a Cox-regression to adjust for confounders were calculated.</p> <p>Results</p> <p>Neurological outcome significantly improved after mild hypothermia treatment (hypothermia group CPC 1-2 59.8%, control group CPC 1-2 24.5%; p < 0.01). In Kaplan-Meier survival analysis hypothermia treatment was also associated with significantly improved 2-year probability for survival (hypothermia 55% vs. control 34%; p = 0.029). Cox-regression analysis revealed hypothermia treatment (p = 0.031) and age (p = 0.013) as independent predictors of 24-month survival.</p> <p>Conclusions</p> <p>Our study demonstrates that the early survival benefit seen with therapeutic hypothermia persists after two years. This strongly supports adherence to current recommendations regarding postresuscitation care for all patients after cardiac arrest due to VF and maybe other rhythms as well.</p

Towards the Evaluation of the Ecological Effectiveness of the Principles, Criteria and Indicators (PCI) of the Forest Stewardship Council (FSC): Case study in the Arkhangelsk Region in the Russian Federation

The Forest Stewardship Council (FSC) is a voluntary sustainability standard with global reach that has been developed to encourage responsible and sustainable forest management. Despite its broad appeal, there is little scientific assessment to substantiate the effectiveness of FSC in the boreal zone. In this study, an ecosystem-based and participatory approach was applied to a case study in the Arkhangelsk Region of the Russia Federation to assess the potential influence of the principles, criteria and indicators of the Russian FSC standard. An ECOSEFFECT theoretical plausibility analysis was conducted to evaluate the potential effectiveness of FSC in safeguarding the ecological integrity of the ecosystem. Besides spatial analysis and a field visitation, core elements of the methodological procedure were workshops with experts and stakeholders who directly contributed to knowledge mapping and analysis. The results of the study suggest FSC can potentially influence and improve forest management including monitoring and evaluation, foster the institutional capacity, and enhance knowledge on the impacts of forest management. Theoretically, FSC has a certain potential to reduce a range of anthropogenic threats to the ecosystem, such as large-scale deforestation and forest degradation, logging of High Conservation Value Forests, large size of clear-cuts, excessive annual allowable cuts, damage to trees during forest operations, and hydrological changes. However, human-induced fire is the only ecological stress that was assumed to be effectively tackled through a strong and positive influence of FSC. The results of the theoretical analysis with a semi-quantitative evaluation revealed the potential for FSC to generate much more effective outcomes for biodiversity by prudently targeting key ecological problems. The biggest problem is the large-scale clear-cutting practice, especially within IFL. These devastating practices are not promoted by, but are compliant with the current Russian FSC standard. This feeds doubts about the consistency of FSC practice and its credibility

Оптимизация работы установок электроцентробежных насосов в процессе добычи нефти на Снежном нефтегазоконденсатном месторождении (Томская область)

Объектом исследования является Снежное нефтегазоконденсатное месторождение. Цель работы – изучить оптимизацию работы установок электроцентробежных насосов в процессе добычи нефти на Снежном нефтегазоконденсатном месторождении. В процессе работы были рассмотрены причины отказов в работе установок электроцентробежных насосов, выполнен статистический анализ отказов в работе скважин, оборудованных УЭЦН, показавший, что большая доля причин проведения подземных ремонтов (до 15%) связана с рассогласованием гидравлических характеристик центробежного насоса и пласта.The object of research is the Snezhnoye oil and gas condensate field. The purpose of the work is to study the optimization of the operation of electric centrifugal pump installations in the process of oil production at the Snezhnoye oil and gas condensate field. During the work, the reasons for failures in the operation of electric centrifugal pump installations were considered, a statistical analysis of failures in the operation of wells equipped with ESP was performed, which showed that a large share of the reasons for underground repairs (up to 15%) is associated with a mismatch in the hydraulic characteristics of the centrifugal pump and the formation