SDS-PAGE gel of moa extractions.

<p>Arrowheads indicate faint bands visible through the smearing.</p

Yields from ∼1.3 g of bone powder of each extraction protocol described in Table 1.

<p>B.D. refers to below the limit of detection for the balance used (0.1 mg). Total values correspond to lyophilized samples only and are calculated by addition of each step of an individual protocol.</p

Summary of Rabilloud method, Craig and Collins method, Embery method, and Schweitzer method.

<p>*Dialysis and lyophilization is abbreviated D/L. Precip represents chloroform∶methanol∶water precipitation performed on half of the supernatant. Volumes correspond to the number of milliliters of buffer multiplied by the grams of bone powder.</p

Moa enzyme-linked immunosorbent assay results showing means plus or minus one standard deviation.

<p>Values correspond to absorbance at 405 nm. − represents no detected absorption. + represents at least two times the average absorbance of buffer control.</p

Summary of Jiang method and Buckley method.

<p>*Dialysis and lyophilization is abbreviated D/L. Volumes correspond to the number of milliliters of buffer multiplied by the grams of bone powder.</p

Summary of Franzén and Heinegård methods.

<p>*Dialysis and lyophilization is abbreviated D/L. Precip represents chloroform∶methanol∶water precipitation performed on half of the supernatant. Volumes correspond to the number of milliliters of buffer multiplied by the grams of bone powder.</p