7 research outputs found
Radiological analysis and callus formation (Cohorts 1, 2, and 7).
<p>Only infectious Cohorts 2 and 7 are shown for comparison while Cohort 1 is included as a non-infection comparator. The presence of a distinct boney callus in Cohorts 1 and an incomplete bridging callus in Cohort 7 are indicative of bone healing. Although soft tissue inflammation cannot be quantitatively assessed, there appeared to be little or no soft tissue inflammation in Cohort 7 compared to Cohort 1, indicating a relatively normal bone healing response. Cohort 2 shows pronounced signs of infection as the device is displaced (indicated by a red arrow).</p
Schematic describing the surgical procedure for the rabbit radial BVF implant.
<p>Schematic describing the surgical procedure for the rabbit radial BVF implant.</p
Modified Petty scale to assess infection (i.e., external inflammation, soft tissue inflammation, bone necrosis, and osteomyelitis) during post-operative monitoring [42].
<p>Modified Petty scale to assess infection (i.e., external inflammation, soft tissue inflammation, bone necrosis, and osteomyelitis) during post-operative monitoring [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118696#pone.0118696.ref042" target="_blank">42</a>].</p
Microbiology assessments post euthanasia.
<p>Data represent the number of animals with culture positive swabs. No bacteria were detected in the blood of any animals. Only infected cohorts are shown with the exception of Cohort 1, which was included as a non-infection comparator. Note that Cohort 2 animals were euthanized prior to all other cohorts due to evident localized infection.</p
Semi-quantitative histology scoring.
<p>Histology images (20x) were obtained from each rabbit and assessed based on the capsule thickness (A), qualitative bone reaction (B), periprosthetic inflammation (C), and bone ingrowth (D). * significance p ≤ 0.05</p
Sanderson’s Rapid Bone Stain (Cohorts 2 and 7).
<p>Two images are shown for each cohort to represent intrinsic variability in bone response to treatment. Soft tissue (blue), presumably fibrous capsule, immune cells, and infection are present in Cohort 2, but mostly lacking in Cohort 7. (2X magnification) BVF—Bone void filler, HB—Host bone. Insets are at 10X magnification.</p
Cohort designs for rabbit radial window defects implanted with bone graft with or without tobramycin and with or without <i>S</i>. <i>aureus</i>.
<p>* Data not presented in this manuscript.</p><p>Cohort designs for rabbit radial window defects implanted with bone graft with or without tobramycin and with or without <i>S</i>. <i>aureus</i>.</p