Mediators secreted 8 or 24(I:C) or HDM.

<p>Cell-free supernatants were analyzed for the presence of cytokines and chemokines. The first part of the table shows mediators induced by 8 or 24 hours stimulation with both HDM and poly(I:C). In the second part, mediators induced only by poly(I:C) was collected. The bottom of the table shows a cytokine which was not affected by either of the stimuli. The concentration values are given in pg/mL and standard deviations are in brackets and represent the average of three biological replicates. Fold changes (FC) were calculated as a ratio between stimulated and control cells and if significant (p<0.05), marked with (^#). Significant differences (p<0.05) between levels of induction by HDM and poly(I:C) are marked with (*). B.d – below the detection limit.</p

Mediators secreted 8h or 24h after poly(I:C) stimulation alone or additionally pre-exposed for 24 hours to HDM.

<p>Cell-free supernatants were analyzed for the presence of cytokines and chemokines in relation to stimulation by poly(I:C) with or without HDM pre-exposure. The table shows cytokines that are: HDM-enhanced in response to poly(I:C); HDM-down-regulated in response to poly(I:C) and not affected by HDM pre-exposure. The concentration values are given in pg/mL and standard deviations are in brackets and represent an average of a triplicate experiment. Fold changes (FC) were calculated as a ratio between HDM pre-stimulation followed by poly(I:C) exposure condition to HBSS pre-exposure followed by poly(I:C) stimulation condition. Fold changes and values were considered significant if p<0.05. Statistically significant induction of cytokines production by poly(I:C) compared to non-induced control conditions is marked with (^#) and significant effect of HDM pre-exposure to the FC values are marked with (*). B.d – below the detection limit.</p

Detailed expression analysis of selected genes in response to poly(I:C) challenge.

<p>The NCI-H292 cell line was stimulated with poly(I:C) in a time course over 24 hours and expression profiles of: <i>ATF3</i>, <i>EGR1</i>, <i>DUSP1</i>, <i>FOS</i>, <i>FOSL1</i>, <i>MYC</i>, <i>JUN</i>, <i>IL6</i>, <i>NKB1,</i> and <i>NFKB2</i> were investigated. Graphs show genes that are: A) induced rapidly after stimulation with poly(I:C); B) induced 2 hours after poly(I:C); C) late-induced; D) not affected by poly(I:C) stimulation. Each time point represents an average of three biological replicates ± standard deviation.</p

Network interaction model of the genes that make up the core response of airway epithelial cells upon HDM allergen exposure.

<p>The principal cellular locations of the gene products (extra cellular region, membrane, cytoplasm, and nucleus) are indicated and their transcriptional interaction.</p