Framing the “Refugee Hunter”: Gender and Nationalist Perspectives on Border Vigilance in Bulgaria

In this article, I explore the construction of the “refugee crisis” from the perspective of border vigilantes in Bulgaria. Drawing on ethnography in Harmanli, a border town with a refugee camp, the article explores how the identity and agency of the “refugee hunter” emerged. I argue that the gendered identity of the “refugee hunter” combines a national feminized victim and a vigilant masculinized protector. The masculinized protector patrols the Bulgarian-Turkish border in order to defend the victimized national community from the immigrant Other and the nongoverning state. The article illustrates that the refugee hunter identity has produced a new mode of hegemonic masculinity, where immigrant men and women are constructed as criminals, while men’ border patrols as heroic

Времето и часот - контрастивна анализа во македонскиот, српскиот и германскиот јазик

Времето и изразувањето на времето во часови се елементи кои ја отсликуваат културата на еден народ и затоа треба на истото да се обрне внимание при изучувањето на часот како на мајчин така и на странски јазик. Голема е веројатноста изучувачите на странскиот јазик да го користат времето и изразувањето на времето во часови при секојдневната комуникација. Во ситуации на невладеење на истото, можат да се јават проблеми во комуникацијата. За спречување на истите, наставниците по мајчин и странски јазик треба да овозможат правилно совладување и примена на времето изразено во часови на мајчин и странски јазик

Feeding of lambs with improved pelleted feed

The feed costs are the major expenses for the farmers and the main input that influenced the economics of livestock production. The physical form of diet is one of the main drivers for better digestibility of feed and improved growth performance of farm animals. This study aimed to evaluate the effect of using two different physical forms of diet (ground feed mixtures and pelleted feed mixtures) on the growth performance of fattening lambs. Seventy-two fattening lambs were randomly assigned to two groups with 36 fattening lambs to assess the effects of feeding with pelleted (test group) and ground feed mixtures (control group) on growth performance. Two physical forms (pelleted vs. ground) were equal in ingredient and chemical composition. The duration of the experiment was 42 days. At the beginning of the trial, the lambs in the test group had lower average live body weight and daily gain compared with the lambs in the control group. However, at the end of the feeding trial, the lambs feeding with a pelleted feed mixture had achieved a higher average live body weight and better daily gain. The results from the ANOVA for growth performance of lambs depending from the physical form of diet indicate that there was a statistically significant difference (p<0.05) between the test and control groups of lambs in the 5th and the 6th week of the feeding trial. These results support the possibility of using pelleted feeds in intensive fattening of lambs for improving the growth performance, increasing average daily gain and reducing the duration of the fattening period

Dysregulated Antibody, Natural Killer Cell and Immune Mediator Profiles in Autoimmune Thyroid Diseases.

Funder: FP7 Ideas: European Research Council; Grant(s): 278535, 305280, 324400, 315997The pathogenesis of autoimmune thyroid diseases (AITD) is poorly understood and the association between different immune features and the germline variants involved in AITD are yet unclear. We previously observed systemic depletion of IgG core fucosylation and antennary α1,2 fucosylation in peripheral blood mononuclear cells in AITD, correlated with anti-thyroid peroxidase antibody (TPOAb) levels. Fucose depletion is known to potentiate strong antibody-mediated NK cell activation and enhanced target antigen-expressing cell killing. In autoimmunity, this may translate to autoantibody-mediated immune cell recruitment and attack of self-antigen expressing normal tissues. Hence, we investigated the crosstalk between immune cell traits, secreted proteins, genetic variants and the glycosylation patterns of serum IgG, in a multi-omic and cross-sectional study of 622 individuals from the TwinsUK cohort, 172 of whom were diagnosed with AITD. We observed associations between two genetic variants (rs505922 and rs687621), AITD status, the secretion of Desmoglein-2 protein, and the profile of two IgG N-glycan traits in AITD, but further studies need to be performed to better understand their crosstalk in AITD. On the other side, enhanced afucosylated IgG was positively associated with activatory CD335- CD314+ CD158b+ NK cell subsets. Increased levels of the apoptosis and inflammation markers Caspase-2 and Interleukin-1α positively associated with AITD. Two genetic variants associated with AITD, rs1521 and rs3094228, were also associated with altered expression of the thyrocyte-expressed ligands known to recognize the NK cell immunoreceptors CD314 and CD158b. Our analyses reveal a combination of heightened Fc-active IgG antibodies, effector cells, cytokines and apoptotic signals in AITD, and AITD genetic variants associated with altered expression of thyrocyte-expressed ligands to NK cell immunoreceptors. Together, TPOAb responses, dysregulated immune features, germline variants associated with immunoactivity profiles, are consistent with a positive autoreactive antibody-dependent NK cell-mediated immune response likely drawn to the thyroid gland in AITD

Safety and anti-tumour activity of the IgE antibody MOv18 in patients with advanced solid tumours expressing folate receptor-alpha: a phase I trial

All antibodies approved for cancer therapy are monoclonal IgGs but the biology of IgE, supported by comparative preclinical data, offers the potential for enhanced effector cell potency. Here we report a Phase I dose escalation trial (NCT02546921) with the primary objective of exploring the safety and tolerability of MOv18 IgE, a chimeric first-in-class IgE antibody, in patients with tumours expressing the relevant antigen, folate receptor-alpha. The trial incorporated skin prick and basophil activation tests (BAT) to select patients at lowest risk of allergic toxicity. Secondary objectives were exploration of anti-tumour activity, recommended Phase II dose, and pharmacokinetics. Dose escalation ranged from 70 μg–12 mg. The most common toxicity of MOv18 IgE is transient urticaria. A single patient experienced anaphylaxis, likely explained by detection of circulating basophils at baseline that could be activated by MOv18 IgE. The BAT assay was used to avoid enrolling further patients with reactive basophils. The safety profile is tolerable and maximum tolerated dose has not been reached, with evidence of anti-tumour activity observed in a patient with ovarian cancer. These results demonstrate the potential of IgE therapy for cancer

Activity of tafasitamab in combination with rituximab in subtypes of aggressive lymphoma

BackgroundDespite recent advances in the treatment of aggressive lymphomas, a significant fraction of patients still succumbs to their disease. Thus, novel therapies are urgently needed. As the anti-CD20 antibody rituximab and the CD19-targeting antibody tafasitamab share distinct modes of actions, we investigated if dual-targeting of aggressive lymphoma B-cells by combining rituximab and tafasitamab might increase cytotoxic effects.MethodsAntibody single and combination efficacy was determined investigating different modes of action including direct cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) in in vitro and in vivo models of aggressive B-cell lymphoma comprising diffuse large B-cell lymphoma (DLBCL) and Burkitt lymphoma (BL).ResultsThree different sensitivity profiles to antibody monotherapy or combination treatment were observed in in vitro models: while 1/11 cell lines was primarily sensitive to tafasitamab and 2/11 to rituximab, the combination resulted in enhanced cell death in 8/11 cell lines in at least one mode of action. Treatment with either antibody or the combination resulted in decreased expression of the oncogenic transcription factor MYC and inhibition of AKT signaling, which mirrored the cell line-specific sensitivities to direct cytotoxicity. At last, the combination resulted in a synergistic survival benefit in a PBMC-humanized Ramos NOD/SCID mouse model.ConclusionThis study demonstrates that the combination of tafasitamab and rituximab improves efficacy compared to single-agent treatments in models of aggressive B-cell lymphoma in vitro and in vivo

Early mobilisation in critically ill COVID-19 patients: a subanalysis of the ESICM-initiated UNITE-COVID observational study

Background Early mobilisation (EM) is an intervention that may improve the outcome of critically ill patients. There is limited data on EM in COVID-19 patients and its use during the first pandemic wave. Methods This is a pre-planned subanalysis of the ESICM UNITE-COVID, an international multicenter observational study involving critically ill COVID-19 patients in the ICU between February 15th and May 15th, 2020. We analysed variables associated with the initiation of EM (within 72 h of ICU admission) and explored the impact of EM on mortality, ICU and hospital length of stay, as well as discharge location. Statistical analyses were done using (generalised) linear mixed-effect models and ANOVAs. Results Mobilisation data from 4190 patients from 280 ICUs in 45 countries were analysed. 1114 (26.6%) of these patients received mobilisation within 72 h after ICU admission; 3076 (73.4%) did not. In our analysis of factors associated with EM, mechanical ventilation at admission (OR 0.29; 95% CI 0.25, 0.35; p = 0.001), higher age (OR 0.99; 95% CI 0.98, 1.00; p ≤ 0.001), pre-existing asthma (OR 0.84; 95% CI 0.73, 0.98; p = 0.028), and pre-existing kidney disease (OR 0.84; 95% CI 0.71, 0.99; p = 0.036) were negatively associated with the initiation of EM. EM was associated with a higher chance of being discharged home (OR 1.31; 95% CI 1.08, 1.58; p = 0.007) but was not associated with length of stay in ICU (adj. difference 0.91 days; 95% CI − 0.47, 1.37, p = 0.34) and hospital (adj. difference 1.4 days; 95% CI − 0.62, 2.35, p = 0.24) or mortality (OR 0.88; 95% CI 0.7, 1.09, p = 0.24) when adjusted for covariates. Conclusions Our findings demonstrate that a quarter of COVID-19 patients received EM. There was no association found between EM in COVID-19 patients' ICU and hospital length of stay or mortality. However, EM in COVID-19 patients was associated with increased odds of being discharged home rather than to a care facility. Trial registration ClinicalTrials.gov: NCT04836065 (retrospectively registered April 8th 2021)

The importance of listening and comprehension skills in listening excercises in German language classes

This study explores the listening and comprehension skills in German language class exercises, in the Republic of Macedonia. The primary and secondary level curriculum, that the Bureau of Education Development in the Ministry of Education of Republic of Macedonia has put forward, describes the presence of listening skills in German language classes of all levels. The same curriculum forms a basis for the discussion regarding the presence of listening skills in German language classes. The types of listening play a big part in the application of listening exercises. The tasks of great importance to the German teachers are: raising the students’ expectations and explaining the procedure of the listening exercise to the students. At the end of this text recommendations regarding raising the listening interest, improving the listening process and developing listening competency are given