Normalized expression profiles of the (A) miR-17-92 cluster and its (B) paralogs in five precursor B cell subsets in adults (solid lines) and children (dotted lines).

<p>All 10 microRNAs followed the same expression profile in both age groups and showed a near inverse pattern with age.</p

Principle component analysis (PCA) of regulated genes.

<p>The clustering represents the overall expression pattern of significantly regulated mRNAs at FDR 0.1% (p-value ≤1.13×10⁻⁴) in five subsets of precursor B cells. Color codes represent the various maturation stages as indicated under the plot. The Partek® Genomics Suite™ program draws the elipsoids encompassing the individual datapoints. Note the dots for the children (spheres) and adults (angular balls) are tightly grouped together.</p

Functional analysis of 1796 differentially expressed mRNAs (FDR 0.1%, p-value≤1.13×10⁻⁴).

<p>Functional analysis of 1796 differentially expressed mRNAs (FDR 0.1%, p-value≤1.13×10⁻⁴).</p

MicroRNA profiles of precursor B cell subsets.

<p>Principle component analysis (PCA) showing the overall expression pattern of 17 microRNAs (18 assays) that were at least once differentially expressed between the various subsets (FDR 10%, p≤0,004). The color codes indicating differentiation stage (right) and age group (left) are explained below.</p

Functional network of up-regulated mRNAs (red) and down-regulated (green) microRNAs during differentiation to PreBII large cells in adults.

<p>Note connection of miR-125b-5p to ID2 and involvement of the hematopoiesis related miR-181a-5p and miR-196a-5p, and the cell cycle regulating miR-24-3p. Note, all the coloured interacting partners in this network were detected in the present study.</p

Cell sorting of precursor B cells subsets from CD10 positively selected cells.

<p>Immunomagnetic selection and subsequent FACS were used to isolate the five populations from pediatric and adult human BM. Shown are the FACS dot plots with sorting gates to obtain CD34⁺CD19⁻ ProB cells, CD34⁺CD19⁺ PreBI cells, CD34⁻CD19⁺CD20^dim PreBII large cells, CD34⁻CD19⁺CD20⁻ PreBII small cells, and CD34⁻CD19⁺CD20^highIgM⁺ Immature B cells.</p

Additional file 3: of Chromosomal copy number alterations for associations of ductal carcinoma in situ with invasive breast cancer

Performance of cytogenetic combinations as predictors of invasive cancer. (DOC 55 kb