38 research outputs found

    Pesticide-degrading organisms and their application in the treatment of spray residues of crop protection products

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    Samenvatting... I Summary ... III List of abbreviations... V List of symbols and corresponding units ... V Chapter I Pesticide biodegradation in on-farm biopurification systems: general background and research objectives ...1 Chapter II Assessment of the success rate of a classical procedure to enrich and isolate pesticide-degrading bacteria from pesticide-primed ecosystems...27 Chapter III Linuron-primed soil as supplement for on-farm biopurification systems to improve linuron mineralization: a microcosm study...41 Chapter IV Stresses sequentially imposed by different environmental perturbations only moderately affect the linuron mineralization capacity of on-farm biopurification systems ...59 Chapter V Inverse modeling of linuron degradation and linuron-degrading population size dynamics in a bioremediation system: parameterizing the Monod model ...77 Chapter VI Required inoculum density to enhance the linuron mineralization capacity in on-farm biopurification systems by bioaugmentation using linuron-primed soil ...89 Chapter VII Assessment of the pesticide degradation and mineralization capacity of biopurification system microcosms treating complex pesticidecontaminated waste water and inoculated with appropriate pesticideprimed materials ...99 Chaptterr VIII Comparison between two bioaugmentation strategies to enhance pesticide mineralization in on-farm biopurification systems: initial establishment and long-term robustness...129 Chapter IX General discussion and research perspectives ...157 Appendix...171 References ...179 List of publications ...191nrpages: 191status: publishe

    Establishment of multiple pesticide biodegradation capacities from pesticide-primed materials in on farm biopurification system microcosms treating complex pesticide-contaminated waste water

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    BACKGROUND: On-farm biopurification systems (BPSs) treat pesticide-containing wastewater at farms by biodegradation and sorption processes. The inclusion of pesticide-primed material carrying a pesticide-degrading microbial community is beneficial for improving biodegradation, but no data exist for treating wastewater containing multiple pesticides, as often occurs on farms. In a microcosm set-up, an examination was carried out to determine whether multiple pesticide degradation activities could be simultaneously established in the matrix of a BPS by the simultaneous inclusion of different, appropriate pesticide-primed materials. The microcosms were fed with a mixture of pesticides including the fungicide metalaxyl and the herbicides bentazon, isoproturon, linuron and metamitron, and pesticide-degrading activities were monitored over time. RESULTS: The strategy immediately provided the microcosms with a multiple pesticide degradation/mineralisation capacity, which improved during feeding of the pesticide mixture. Not only did the degradation of the parent compound improve but also that of the produced metabolites and compound mineralisation. The time to achieve maximum degradation/mineralisation capacity depended on the pesticide degradation capacity of the pesticide-primed materials. CONCLUSIONS: The data obtained show that the addition of pesticide-primed materials into the matrix of a BPS as an approach to improve biodegradation can be extended to the treatment of pesticide mixtures.status: publishe

    Cyclodextrines accelerate off-site bioremediation of soils contaminated with mineral oil

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    Soils contaminated with mineral oil are often excavated and bioremediated off-site by biostimulation and biodegradation. The soils are aerated and supplied with the necessary nutrients to accelerate biodegradation. Ideally, without seriously disturbing the soil, the contaminants are completely mineralized and the soil can be reused. However, for some soils the degradation performance is poor despite the sufficient amount of bacteria, oxygen and nutrients present in the soil. The main factor is the limited bioavailability of the mineral oili. As a result, the environmental permits cannot be achieved within a reasonable time period. The use of additives such as cyclodextrin can enhance the bioavailability of the oil and therefore accelerate degradationii. Cyclodextrin is a (α-1,4)-linked oligosaccharide with both an apolar cavity and polair exterior. They are able to encapsulate mineral oil thereby enhancing the solubility and therefore the bioavailability.status: publishe
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