Variation of Pigment and Anthocyanin Content of Local Black Rice From YOGYAKARTA on Two Altitude

There are several types of local black rice with different quality which well known in Yogyakarta. However, the altitude location affect to the quality and pigmentation of local black rice remains were unknown. The objectives of this research was to determine the variation of rice pigmentation and total anthocyanin content of Yogyakarta local black rice which planted on two differentaltitudes location. This study was conducted from February to December 2015 with the experimental design using factorial experiment. The first factor is seven local black rice varieties from Yogyakarta (G) and second factor is two different altitude locations: lowlands (26 m asl) of Gulon, Sri Hardono, Pundong, Bantul (A1), and in medium lands (462 m asl) of Padasan, Pakembinangun, Pakem, Sleman (A2). The total anthocyanin content and rice pigmentation were observed. Three color variables for rice pigmentation, i.e. lightness (L*), greenness (a*), and yellowness (b*) were measured by Chroma Meter, Konica Minolta– Minolta CM-2006. The results showed that there was diversity on pigmentation and total anthocyanin content in the black rice grown at different altitude. Local variety Tugiyo Umur Panjang has any anthocyanin highest in the low altitude location (Bantul), while local variety Sembada Hitam was showed the highest anthocyanin at the height of the medium (Sleman). A positivesignificant correlation was found among color variables, i.e. lightness (L*), greenness (a*), and yellowness (b*) and there was a negative significant correlation between total anthocyanin content and L*, a*, and b* color variables

Mutu Fisik, Mutu Giling Dan Mutu Fungsional Beras Varietas Lokal Kalimantan Barat

The physical of paddy and milled rice qualities, physicochemical properties and the functional properties of milled rice were analyzed to study the grain characteristics of four local paddy varieties derived from West Kalimantan. The study was conducted at Post Harvest Grain Quality Laboratory, Indonesian Center for Rice Research in 2013. Method used for physical and milling quality properties followed IRRI method. Functional characteristic was analyzed using LC-MS. The observations were done in six replications. The data was analyzed for the correlation among characters. Significant correlation between characters was further analysed for regression equation. The shapes of grains of local rice varieties were slim to medium. There was correlation between paddy moisture content and head rice percentage, between damaged grain and paddy density, between yield of brown rice and yield of milled rice, and percentage of head rice, between percentage of head rice and yield of milled rice, and between empty grain and broken grain. The functional character relationship showed that Cyanidin 3 Glucosidase (C3G) content of brown rice influenced the C3G content of milled rice. “Sanik” red rice, “Beliah” purple rice and “Balik” black rice may be useful to be used as parent for crossing in the breeding program for functional rice varieties, due to their good quality of milled rice and their high content of C3G. The C3G is considered as anticancer, antioxidant, anti coronaria heart disease and it improves fat profiles in the blood. Therefore, colored rice is recommended to be consumed in a form of brown rice or milled rice with 80% degree milling to retain the C3G content which is beneficial for the human health

Kemajuan Genetik Dan Heritabilitas Karakter Agronomi Padi Beras Hitam Pada Populasi F2

Black rice is a local rice variety with some advantages and weaknesses. The desirable character of black rice is its high anthocyanin content, while the weaknesses are late maturing, tall plant stature and low grain yield. Crosses were made between two parents aimed to recombine the superior traits. The effectiveness and efficiency of selection would be determined by the magnitude of the heritability of traits and the selection progress. This study was aimed to determine the heritability estimates in a broad sense and the genetic advance of agronomic characters of F2 generation, from crosses of local black rice and high yielding variety of white rice. The experiment was conducted in Padasan, Pakembinangun, Pakem, Sleman, Yogyakarta from January to May 2013. The F2 population derived from crosses of black rice x white rice, with the morphological traits of hairless black rice (S) crossed with Situbagendit (G) white rice, Cempo ireng (C) with Situbagendit (G) and Cempo ireng (C) with Inpari 6 (I). The F2 plants population were planted without replication. Genetic parameters estimated were calculated for broad sense heritability and genetic advance from selection. Results showed that the F2 population had high broad sense heritability estimates for all characters observed, including: plant height, flag leaf length, number of productive tillers, panicle length, number of filled grains per panicle, number of empty grains per panicle, maturity and rice color, in the three F2 populations obtained from crosses of S x G, C x G and C x I. Only the plant height of S x G cross had a medium broad sense heritability estimate. The large heritability estimates indicated that the respective character was controlled by genetic factor more than environmental ones. High genetic advance would be obtained in the flag leaf length, number of productive tillers, panicle length, number of filled grains/panicle, number of empty grains/panicle and rice color from S x G, C x G and C x I crosses. Genetic advance of plant height was medium (at S x G and C x I crosses) to high (C x G crosses). The maturity trait had low genetic advance on cross S x G; C x G; and Cx I. The high value of genetic advance of character would suggest that selection on the character\u27s appearance would be successful

High Resolution Microsatellite Marker Analysis of Some Rice Landraces Using Metaphor Agarose Gel Electrophoresis

Microsatellite markers or simple sequences repeats are DNA - based molecular techniques that areused to see the different among accessions and inbred lines. There are three methods to analysis the results ofthe polymerase chain reaction of microsatellite markers namely polyacrylamide gel electrophoresis (PAGE),capillary electroforesis, and Metaphor Agarose Gel Electroforesis (MAGE), and the Use of MAGE assessedmore easily and economically the polymorphic pattern of DNA markers. This study aimed to obtain fast,effective and efficient in term of easy and cheap technique to identify microsatellite markers of some blackrice cultivars and F2 populations from crosses between black with white rice. The results showed that MAGEsuccessfully separated clearly SSRs alleles with different sizes of less than 25 bp

Suitability of the Cropping System Some Varieties of Maize (Zea Mays L.) and Soybean (Glycine Max L.) with Intercropping System on Dry Lands

Intercropping is an attempt from agriculture's intensification program which purposes to obtain optimum yield and maintain soil's fertility. This research aims to know whether intercropping systems have better crops and higher yields than monoculture systems. This research was conduct on May-August 2020 at Trengguno Kidul, Sidorejo, Ponjong, Gunung Kidul. The design of the experiment was arranged in a randomized block design (RBD) one factor and three blocks. One factor namely ST 1: soybean monoculture Dega variety, ST2: maize monoculture Dega 1 variety, ST 3: Maize monoculture Srikandi Ungu variety, ST 4: Intercropping system of soybean Dega 1variety with maize Pulut Uti 1 variety, ST 5: Intercropping system of soybean Dega 1 variety and maize Nasa 29 variety, ST 6: Intercropping system of soybean Dega 1 variety with maize Pulut Uri 1 variety, ST 7: Intercropping system of soybean Dega 1 variety and maize Srikandi Ungu variety. Data were analyzed statistically using SAS (Statistical Analysis System) ANOVA 5% and further tested with the Duncan test at a significant level of 5%. The results show soybean on monoculture system has better growth and yield than intercropping while on maize of Pulut Uri and Nasa 29 plant intercropping system has the same growth and yield than monoculture. Combination intercropping of maize variety namely Srikandi Ungu and Soybean variety namely Dega proved better growth and yield

The Resistance of Local Pigmented Rice Varieties Against Bacterial Leaf Blight

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important disease in rice plants worldwide, including in Indonesia. The use of resistant variety against BLB is the best effort in disease control because it is considered as the most effective and environmentally friendly. This study aimed to evaluate the resistance of local varieties, red rice (cv. Sembada Merah) and black rice (cv. Sembada Hitam), against Xoo compared to white rice variety (Ciherang), commonly cultivated by the farmers. The study was conducted in a greenhouse and arranged in Factorial of Completely Randomized Design (CRD) with 5 replications and 2 treatment factors: variety (Sembada Hitam, Sembada Merah, and Ciherang) and inoculation (with and without inoculated by Xoo bacterial suspension). Xoo inoculation was performed by clipping the leaves that close to the reproductive phase when the heading begins to be produced. The disease intensity and Area Under Disease Progress Curve (AUDPC) over two weeks were used to determine the level of resistance of the tested plant varieties. The results showed that black rice and red rice had better levels of resistance against BLB compared to white rice. At two weeks after inoculation, Sembada Hitam had the lowest AUDPC value followed by Sembada Merah, while the Ciherang had the highest AUDPC value and was categorized as a susceptible variety to Xoo

Effects of Heavy Metal-Tolerant Microorganisms on the Growth of “Narra” Seedlings

The effectiveness of heavy metal-tolerant microorganisms for supporting plant growth needs to be understood before it can be used as a soil bioremediation agent. The purpose of this study was to determine the effect of heavy metal tolerant microorganisms on the growth of “Narra” seedling (Pterocarpus indicus Wild). Three heavy metals-resistant (Pb, Cd, and Cu) rhizobacteria from a copper (Cu) mined-out site in Marinduque, Philippines showed plant growth promotion in vitro. A treatment combination of formula inoculant A (CuNFbM 4.1, MGR 333), B (CuNFbM 4.1, MGR 333, PbSM 2.1), and O (Uninoculated); compost (0%, 4%); and lime + inorganic fertilizer {without or with lime and inorganic fertilizer (LF0; LF1)} were applied to Narra seedlings planted on 445 mg/kg Cu-contaminated soil. Lime (2 mg/ha) and the recommended dose of soybean inorganic fertilizer were used as positive controls to evaluate the ability of inoculations and composts to promote the growth and used as positive controls to evaluate the ability of inoculants and composts to promote the growth and copper accumulation of narra in greenhouse experiments. All treatment combinations resulted in significant differences in plant height, leaf number, stem diameter, shoot and root dry weight, as well as, shoot, root Cu content, and plant Cu uptake of 13-week-old “Narra”. Inoculated “Narra” could thrive better in mine-degraded soil containing 445 ppm Cu with 4% compost. Inoculant B demonstrated the best plant performance while Pseudomonas synxantha (PbSM 2.1) probably increases the plant’s growth due to 1-aminocyclopropane-1-carboxylate (ACC) deaminase it produces. Accumulation of Cu was higher in the root compared other plant parts. More research is necessary to elucidate the mechanism of plant growth promotion and heavy metal re mediation by P. synxantha

Effects of Heavy Metal-Tolerant Microorganisms on the Growth of “Narra” Seedlings

The effectiveness of heavy metal-tolerant microorganisms for supporting plant growth needs to be understood before it can be used as a soil bioremediation agent. The purpose of this study was to determine the effect of heavy metal tolerant microorganisms on the growth of “Narra” seedling (Pterocarpus indicus Wild). Three heavy metals-resistant (Pb, Cd, and Cu) rhizobacteria from a copper (Cu) mined-out site in Marinduque, Philippines showed plant growth promotion in vitro. A treatment combination of formula inoculant A (CuNFbM 4.1, MGR 333), B (CuNFbM 4.1, MGR 333, PbSM 2.1), and O (Uninoculated); compost (0%, 4%); and lime + inorganic fertilizer {without or with lime and inorganic fertilizer (LF0; LF1)} were applied to Narra seedlings planted on 445 mg/kg Cu-contaminated soil. Lime (2 mg/ha) and the recommended dose of soybean inorganic fertilizer were used as positive controls to evaluate the ability of inoculations and composts to promote the growth and used as positive controls to evaluate the ability of inoculants and composts to promote the growth and copper accumulation of narra in greenhouse experiments. All treatment combinations resulted in significant differences in plant height, leaf number, stem diameter, shoot and root dry weight, as well as, shoot, root Cu content, and plant Cu uptake of 13-week-old “Narra”. Inoculated “Narra” could thrive better in mine-degraded soil containing 445 ppm Cu with 4% compost. Inoculant B demonstrated the best plant performance while Pseudomonas synxantha (PbSM 2.1) probably increases the plant’s growth due to 1-aminocyclopropane-1-carboxylate (ACC) deaminase it produces. Accumulation of Cu was higher in the root compared other plant parts. More research is necessary to elucidate the mechanism of plant growth promotion and heavy metal re mediation by P. synxantha