THE DEVELOPMENT OF CHITOSAN NANOPARTICLES FROM HIBISCUS SABDARIFFA L CALYX EXTRACT FROM INDONESIA AND THAILAND

Rosella (Hibiscus sabdariffa L) calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP) to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM) and particle size analyzer (PSA). We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan in acetate buffer pH 4. The characteristic of nanoparticles were spherical shapes with particles size range 60 to 300 nm, polydispersity index around 0.3 and zeta potential 39.19 to 62.26 mV. The nanoparticles has high entrapment efficiency of flavonoid active compound of 81.98 to 83.83%

CEK SIMILARITY "THE DEVELOPMENT OF CHITOSAN NANOPARTICLES FROM HIBISCUS SABDARIFFA L CALYX EXTRACT FROM INDONESIA AND THAILAND"

Rosella (Hibiscus sabdariffa L) calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP) to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM) and particle size analyzer (PSA). We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan in acetate buffer pH 4. The characteristic of nanoparticles were spherical shapes with particles size range 60 to 300 nm, polydispersity index around 0.3 and zeta potential 39.19 to 62.26 mV. The nanoparticles has high entrapment efficiency of flavonoid active compound of 81.98 to 83.83%

Cek Similarity "Antioxidant Activity Of Nanoparticle From Rosella (Hibiscus sabdariffa L)Calyx Extract Originated Indonesia And Thailand"

Rosella (Hibiscus sabdariffa) contains flavonoids with antioxidant activity. The differences of geographical location affect secondary metabolites production of rosella calxy. Antioxidant activity determination by thiobarbituric acid reactive subtances (TBARS) assay has been widely used. Design of nanoparticles formulation is needed to improve the physical characteristic and inrease the bioavailability. This study aimed to determine the antioxidant activity of nanoparticles of extract rosella calyx from Thailand and Indonesia.Extraction on rosella were performed by maceration method using ethanol 60% and water. Extract were evaporated to get the concentrated extract and followed freeze dryer. The preparation of nanoparticles used ionic gelation mehode. The nanoparticles were tested in vitro by the thiobarbituric acid reactive subtances (TBARS) assay. Results showed nanoparticles of ethanol extract rosella calxy from Thailand and Indonesia, water extract rosella caly from Thailand and Indonesia 64.3 nm, 101.7nm, 1024.4 nm, and 300.2 nm. nanoparticles of four extract have antioxxidant ability as better than extract from.. Nanoparticles of ethanol extract of roselle calyx from Thailand has the best antioxidant activity. Keywords: antioxidants, Hibiscus sabdariffa, nanoparticles, rosella

Cek Similarity "Antioxidant Activity Of Nanoparticle From Rosella (Hibiscus sabdariffa L)Calyx Extract Originated Indonesia And Thailand"

Rosella (Hibiscus sabdariffa) contains flavonoids with antioxidant activity. The differences of geographical location affect secondary metabolites production of rosella calxy. Antioxidant activity determination by thiobarbituric acid reactive subtances (TBARS) assay has been widely used. Design of nanoparticles formulation is needed to improve the physical characteristic and inrease the bioavailability. This study aimed to determine the antioxidant activity of nanoparticles of extract rosella calyx from Thailand and Indonesia.Extraction on rosella were performed by maceration method using ethanol 60% and water. Extract were evaporated to get the concentrated extract and followed freeze dryer. The preparation of nanoparticles used ionic gelation mehode. The nanoparticles were tested in vitro by the thiobarbituric acid reactive subtances (TBARS) assay. Results showed nanoparticles of ethanol extract rosella calxy from Thailand and Indonesia, water extract rosella caly from Thailand and Indonesia 64.3 nm, 101.7nm, 1024.4 nm, and 300.2 nm. nanoparticles of four extract have antioxxidant ability as better than extract from.. Nanoparticles of ethanol extract of roselle calyx from Thailand has the best antioxidant activity. Keywords: antioxidants, Hibiscus sabdariffa, nanoparticles, rosella

Antioxidant Activity Of Nanoparticle From Rosella (Hibiscus sabdariffa L)Calyx Extract Originated Indonesia And Thailand

Rosella (Hibiscus sabdariffa) contains flavonoids with antioxidant activity. The differences of geographical location affect secondary metabolites production of rosella calxy. Antioxidant activity determination by thiobarbituric acid reactive subtances (TBARS) assay has been widely used. Design of nanoparticles formulation is needed to improve the physical characteristic and inrease the bioavailability. This study aimed to determine the antioxidant activity of nanoparticles of extract rosella calyx from Thailand and Indonesia.Extraction on rosella were performed by maceration method using ethanol 60% and water. Extract were evaporated to get the concentrated extract and followed freeze dryer. The preparation of nanoparticles used ionic gelation mehode. The nanoparticles were tested in vitro by the thiobarbituric acid reactive subtances (TBARS) assay. Results showed nanoparticles of ethanol extract rosella calxy from Thailand and Indonesia, water extract rosella caly from Thailand and Indonesia 64.3 nm, 101.7nm, 1024.4 nm, and 300.2 nm. nanoparticles of four extract have antioxxidant ability as better than extract from.. Nanoparticles of ethanol extract of roselle calyx from Thailand has the best antioxidant activity. Keywords: antioxidants, Hibiscus sabdariffa, nanoparticles, rosella

Hasil Peer Review Teman Sejawat "THE DEVELOPMENT OF CHITOSAN NANOPARTICLES FROM HIBISCUS SABDARIFFA L CALYX EXTRACT FROM INDONESIA AND THAILAND"

Rosella (Hibiscus sabdariffa L) calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP) to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM) and particle size analyzer (PSA). We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan in acetate buffer pH 4. The characteristic of nanoparticles were spherical shapes with particles size range 60 to 300 nm, polydispersity index around 0.3 and zeta potential 39.19 to 62.26 mV. The nanoparticles has high entrapment efficiency of flavonoid active compound of 81.98 to 83.83%

Quantitative analysis of γ–oryzanol content in cold pressed rice bran oil by TLC–image analysis method

Objective: To develop and validate an image analysis method for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. Methods: TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test. Results: Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol. Conclusions: The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil

The Delevopment of chitosan nanoparticles from Hibiscus sabdariffa L Calyx Extract from Indonesia and Thailand

Rosella (Hibiscus sabdariffa L) calyx has been reported to have an antioxidant activity. Antocyanidin is the major compound of rosella and has low bioavailability as an ionic form. Rosella extract is an ucceptable due to high acidity. Development of chitosan nanoparticle from Hibiscus sabdariffa extract were expected to improve the bioavailability and the acidity problem. We studied the optimum pH and composition ratios of chitosan, extract and tripolyphosphate (TPP) to develop chitosan-nanoparticle of rosella extract. We used rosella calyx both from Indonesia and Thailand to compare the physical characteristic the extract and nanoparticles. Rosella was extracted using 60% ethanol and water, followed by evaporation using evaporator and dried by freezedryer. The optimum formation of chitosan-nanoparticles of rosella extract were observed including pH formation and composition ratios between extract: chitosan: TPP. The physical charactersitics of nanoparticles were observed using transmission electron microscope (TEM) and particle size analyzer (PSA). We found that optimum composition of extract: chitosan : TPP ratios were 2 : 1 : 0.1, with optimum formation of chitosan in acetate buffer pH 4. The characteristic of nanoparticles were spherical shapes with particles size range 60 to 300 nm, polydispersity index around 0.3 and zeta potential 39.19 to 62.26 mV. The nanoparticles has high entrapment efficiency of flavonoid active compound of 81.98 to 83.83%

Hasil Peer Review Teman Sejawat "Antioxidant Activity Of Nanoparticle From Rosella (Hibiscus sabdariffa L)Calyx Extract Originated Indonesia And Thailand"

Rosella (Hibiscus sabdariffa) contains flavonoids with antioxidant activity. The differences of geographical location affect secondary metabolites production of rosella calxy. Antioxidant activity determination by thiobarbituric acid reactive subtances (TBARS) assay has been widely used. Design of nanoparticles formulation is needed to improve the physical characteristic and inrease the bioavailability. This study aimed to determine the antioxidant activity of nanoparticles of extract rosella calyx from Thailand and Indonesia.Extraction on rosella were performed by maceration method using ethanol 60% and water. Extract were evaporated to get the concentrated extract and followed freeze dryer. The preparation of nanoparticles used ionic gelation mehode. The nanoparticles were tested in vitro by the thiobarbituric acid reactive subtances (TBARS) assay. Results showed nanoparticles of ethanol extract rosella calxy from Thailand and Indonesia, water extract rosella caly from Thailand and Indonesia 64.3 nm, 101.7nm, 1024.4 nm, and 300.2 nm. nanoparticles of four extract have antioxxidant ability as better than extract from.. Nanoparticles of ethanol extract of roselle calyx from Thailand has the best antioxidant activity. Keywords: antioxidants, Hibiscus sabdariffa, nanoparticles, rosella