Cek Similarity "Antioxidant Activity Of Nanoparticle From Rosella (Hibiscus sabdariffa L)Calyx Extract Originated Indonesia And Thailand"

Abstract

Rosella (Hibiscus sabdariffa) contains flavonoids with antioxidant activity. The differences of geographical location affect secondary metabolites production of rosella calxy. Antioxidant activity determination by thiobarbituric acid reactive subtances (TBARS) assay has been widely used. Design of nanoparticles formulation is needed to improve the physical characteristic and inrease the bioavailability. This study aimed to determine the antioxidant activity of nanoparticles of extract rosella calyx from Thailand and Indonesia.Extraction on rosella were performed by maceration method using ethanol 60% and water. Extract were evaporated to get the concentrated extract and followed freeze dryer. The preparation of nanoparticles used ionic gelation mehode. The nanoparticles were tested in vitro by the thiobarbituric acid reactive subtances (TBARS) assay. Results showed nanoparticles of ethanol extract rosella calxy from Thailand and Indonesia, water extract rosella caly from Thailand and Indonesia 64.3 nm, 101.7nm, 1024.4 nm, and 300.2 nm. nanoparticles of four extract have antioxxidant ability as better than extract from.. Nanoparticles of ethanol extract of roselle calyx from Thailand has the best antioxidant activity. Keywords: antioxidants, Hibiscus sabdariffa, nanoparticles, rosella