Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron-2

Itional 16 weeks in four separate experiments. Media additions during the promotion phase and the number of replicates were: 200 μM ammonium citrate (AmCit), n = 4 (- MNNG), n = 4 (+ MNNG); 200 μM ferric ammonium citrate (FAC), n = 3 (- MNNG), n = 4 (+ MNNG); or 200 μM ferric ammonium citrate,160 μM desferoxamine (FAC, dfo), n = 2 (+ MNNG). Cell transformation was assayed by growth in soft agar as described under Methods. Mean values and standard errors from up to four separate experiments are shown (*p<p><b>Copyright information:</b></p><p>Taken from "Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron"</p><p>http://www.biomedcentral.com/1471-230X/8/2</p><p>BMC Gastroenterology 2008;8():2-2.</p><p>Published online 6 Feb 2008</p><p>PMCID:PMC2275280.</p><p></p

Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron-1

concentrations were: none (open bars), 50 μM (light stipled bars), 100 μM (medium stipled bars), 200 μM (dark stipled bars), and 500 μM (solid bars). . . Triplicate wells were harvested and counted after 3 days or after 7 days (with one renewal of FAC in fresh media). The dashed line indicates the plating density of 40,000 cells per well. . . Triplicate wells were assayed using MTT after 5 days as described under Methods. In all panels the means and standard errors determined from three separate experiments are shown (*p<p><b>Copyright information:</b></p><p>Taken from "Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron"</p><p>http://www.biomedcentral.com/1471-230X/8/2</p><p>BMC Gastroenterology 2008;8():2-2.</p><p>Published online 6 Feb 2008</p><p>PMCID:PMC2275280.</p><p></p

Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron-0

200 μM FAC and 160 μM desferoxamine (FAC + dfo) for 5 days. Total non-heme iron (nmol/mg cell lysate protein) was determined by a ferrozine-based colorimetric assay as described under Methods. Values are reported as the means +/- s.e.m. of triplicate dishes (**p < 0.001 compared to control). . . Cells were pulsed with calcein-AM for 30 minutes, rinsed, and incubated for 2 days in complete cell media containing: no addition, 200 μM FAC, or 200 μM FAC and 160 μM dfo. Identical fields for FITC fluorescence (left panels; corresponding to calcein signal) and phase contrast (right panels) are shown. . . Cells were treated with 0, 200, or 500 μM FAC for 5 days and processed for western blots using antibodies specific for ferritin L, ferritin H, or GAPDH as gel loading control. Each experiment was performed at least twice with similar results.<p><b>Copyright information:</b></p><p>Taken from "Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron"</p><p>http://www.biomedcentral.com/1471-230X/8/2</p><p>BMC Gastroenterology 2008;8():2-2.</p><p>Published online 6 Feb 2008</p><p>PMCID:PMC2275280.</p><p></p

Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron-4

200 μM FAC and 160 μM desferoxamine (FAC + dfo) for 5 days. Total non-heme iron (nmol/mg cell lysate protein) was determined by a ferrozine-based colorimetric assay as described under Methods. Values are reported as the means +/- s.e.m. of triplicate dishes (**p < 0.001 compared to control). . . Cells were pulsed with calcein-AM for 30 minutes, rinsed, and incubated for 2 days in complete cell media containing: no addition, 200 μM FAC, or 200 μM FAC and 160 μM dfo. Identical fields for FITC fluorescence (left panels; corresponding to calcein signal) and phase contrast (right panels) are shown. . . Cells were treated with 0, 200, or 500 μM FAC for 5 days and processed for western blots using antibodies specific for ferritin L, ferritin H, or GAPDH as gel loading control. Each experiment was performed at least twice with similar results.<p><b>Copyright information:</b></p><p>Taken from "Neoplastic transformation of rat liver epithelial cells is enhanced by non-transferrin-bound iron"</p><p>http://www.biomedcentral.com/1471-230X/8/2</p><p>BMC Gastroenterology 2008;8():2-2.</p><p>Published online 6 Feb 2008</p><p>PMCID:PMC2275280.</p><p></p