45 research outputs found
57: Predictive Value of MRD Prior to Allogeneic SCT in Relapsed Childhood Acute Lymphoblastic Leukemia – Analysis of the ALL-REZ BFM Group
Sequence polymorphism systems for quantitative real-time polymerase chain reaction to characterize hematopoietic chimerism-high informativity and sensitivity as well as excellent reproducibility and precision of measurement.
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57: Predictive Value of MRD Prior to Allogeneic SCT in Relapsed Childhood Acute Lymphoblastic Leukemia – Analysis of the ALL-REZ BFM Group
Über den Witz der Schizophrenen Eine denkpsychologische und psychopathologische Untersuchung
66: Excellent Engraftment and Rapid Immune Recovery in Haploidentical Stem Cell Transplantation Using CD3/CD19 Depleted Peripheral Stem Cell Grafts After Reduced Intensity Conditioning
Haploidentical stem cell transplantation in children: Improved engraftment and immune recovery after depletion of T and B cells instead of positive selection of stem cells?
Standardization of WT1 mRNA quantitation for minimal residual disease monitoring in childhood AML and implications of WT1 gene mutations: a European multicenter study
The MiR-320 family is strongly downregulated in patients with COVID-19 induced severe respiratory failure
A high incidence of thromboembolic events associated with high mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections with respiratory failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression in relation to activated coagulation and inflammation in 21 critically ill SARS-CoV-2 patients. The cohort consisted of patients with moderate respiratory failure (n = 11) and severe respiratory failure (n = 10) at an acute stage (day 0–3) and in the later course of the disease (>7 days). All patients needed supplemental oxygen and severe patients were defined by the requirement of positive pressure ventilation (intubation). Levels of D-dimers, activated partial thromboplastin time (aPTT), C-reactive protein (CRP), and interleukin (IL)-6 were significantly higher in patients with severe compared with moderate respiratory failure. Concurrently, next generation sequencing (NGS) analysis demonstrated increased dysregulation of miRNA expression with progression of disease severity connected to extreme downregulation of miR-320a, miR-320b and miR-320c. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed involvement in the Hippo signaling pathway, the transforming growth factor (TGF)-β signaling pathway and in the regulation of adherens junctions. The expression of all miR-320 family members was significantly correlated with CRP, IL-6, and D-dimer levels. In conclusion, our analysis underlines the importance of thromboembolic processes in patients with respiratory failure and emphasizes miRNA-320s as potential biomarkers for severe progressive SARS-CoV-2 infection