Master Class as a Basis of Plener Practice

The article presents the pedagogical experience of working on outdoor activities in nature – open air with the use of pedagogical technology – a master class.В статье представлен педагогический опыт работы на выездных занятиях на природе – пленэр с использованием педагогической технологии – мастер-класс

Techno-Economic Analysis of Horseradish Peroxidase Production Using a Transient Expression System in Nicotiana benthamiana

Despite the advantages of plant-based transient expression systems relative to microbial or mammalian cell systems, the commercial production of recombinant proteins using plants has not yet been achieved to any significant extent. One of the challenges has been the lack of published data on the costs of manufacture for products other than biopharmaceuticals. In this study, we report on the techno-economic analysis of the production of a standard commercial enzyme, namely, horseradish peroxidase (HRP), using a transient expression system in Nicotiana benthamiana. Based on the proven plant yield of 240 mg HRP/kg biomass, a biomass productivity of 15-kg biomass/m2/year and a process yield of 54 % (mg HRP product/mg HRP in biomass), it is apparent that HRP can be manufactured economically via transient expression in plants in a large-scale facility (>5 kg HRP/year). At this level, the process is competitive versus the existing technology (extraction of the enzyme from horseradish), and the product is of comparable or improved activity, containing only the preferred isoenzyme C. Production scale, protein yield and biomass productivity are found to be the most important determinants of overall viability.The National Intellectual Property Management Organisation, the Research Contracts and Intellectual Property Services of the University of Cape Town and the Technology Innovation Agency and the Department of Science and Technology.http://link.springer.com/journal/120102016-01-30hj201